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Actin and Actin-Binding Proteins in Cancer Progression and Metastasis

  • Marleen Van Troys
  • Joël Vandekerckhove
  • Christophe Ampe
Part of the Protein Reviews book series (PRON, volume 8)

With over 10 million cases and over 5.7 million deaths a year (GLOBOCAN data 2002; Ferlay et al. 2004), cancer presents a major health problem worldwide. Dealing with cancer, both from a clinical and from a fundamental scientific view, is complicated by the extensive diversity within this disease. Based on their origin, more than 100 different human cancer types have been described and, within one organ, distinct subtypes can occur. In addition, tumors may progress to a malignant state that manifests itself mainly when tumor cells spread out from the primary lesion (or neoplasm) and metastasize, i.e., colonize distant sites of the patient’s body. Metastatic cancer, accounts for 90% of cancer-related lethality (Sporn 1996) and hence forms the primary determining factor in patient outcome. Therefore a detailed molecular understanding of tumor cell spread is required to render treatment of cancer more specific and efficient.

Keywords

Prostate Cancer Actin Filament Actin Cytoskeleton Anaplastic Lymphoma Kinase Anaplastic Large Cell Lymphoma 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Adya, N., Stacy, T., Speck, N. A. and Liu, P. P. 1998. The leukemic protein core binding factor beta (CBFbeta)-smooth-muscle myosin heavy chain sequesters CBFalpha2 into cytoskeletal filaments and aggregates. Mol. Cell. Biol. 18, 7432-7443.PubMedGoogle Scholar
  2. Ahmed, F., Wyckoff, J., Lin, E. Y., Wang, W., Wang, Y., Hennighausen, L., Miyazaki, J., Jones, J., Pollard, J. W., Condeelis, J. S. and Segall, J. E. 2002. GFP expression in the mammary gland for imaging of mammary tumor cells in transgenic mice. Cancer Res. 62, 7166-7169.PubMedGoogle Scholar
  3. Al-Nedawi, K. N., Czyz, M., Bednarek, R., Szemraj, J., Swiatkowska, M., Cierniewska-Cieslak, A., Wyczolkowska, J. and Cierniewski, C. S. 2004. Thymosin beta 4 induces the synthesis of plasminogen activator inhibitor 1 in cultured endothelial cells and increases its extracellular expression. Blood 103, 1319-1324.PubMedGoogle Scholar
  4. Alldinger, I., Dittert, D., Peiper, M., Fusco, A., Chiappetta, G., Staub, E., Lohr, M., Jesnowski, R., Baretton, G., Ockert, D., Saeger, H. D., Grutzmann, R. and Pilarsky, C. 2005. Gene expression analysis of pancreatic cell lines reveals genesoverexpressed in pancreatic cancer. Pancreatology 5, 370-379.PubMedGoogle Scholar
  5. Amsellem, V., Kryszke, M. H., Hervy, M., Subra, F., Athman, R., Leh, H., BrachetDucos, C. and Auclair, C. 2005. The actin cytoskeleton-associated protein zyxin acts as a tumor suppressor in Ewing tumor cells. Exp. Cell Res. 304, 443-456.PubMedGoogle Scholar
  6. Anderson, K. I., Wang, Y. L. and Small, J. V. 1996. Coordination of protrusion and translocation of the keratocyte involves rolling of the cell body. J. Cell Biol. 134, 1209-1218.PubMedGoogle Scholar
  7. Anilkumar, G., Rajasekaran, S. A., Wang, S., Hankinson, O., Bander, N. H. and Rajasekaran, A. K. 2003. Prostate-specific membrane antigen association with filamin A modulates its internalization and NAALADase activity. Cancer Res. 63, 2645-2648.PubMedGoogle Scholar
  8. Asch, H. L., Winston, J. S., Edge, S. B., Stomper, P. C. and Asch, B. B. 1999. Downregulation of gelsolin expression in human breast ductal carcinoma in situ with and without invasion. Breast Cancer Res. Treat. 55, 179-188.PubMedGoogle Scholar
  9. Bagheri-Yarmand, R., Mazumdar, A., Sahin, A. A. and Kumar, R. 2006. LIM kinase 1 increases tumor metastasis of human breast cancer cells via regulation of the urokinase-type plasminogen activator system. Int. J. Cancer 118, 2703-2710.PubMedGoogle Scholar
  10. Bajou, K., Noel, A., Gerard, R. D., Masson, V., Brunner, N., Holst-Hansen, C., Skobe, M., Fusenig, N. E., Carmeliet, P., Collen, D. and Foidart, J. M. 1998. Absence of host plasminogen activator inhibitor 1 prevents cancer invasion and vascularization. Nat. Med. 4, 923-928.PubMedGoogle Scholar
  11. Ballestrem, C., Hinz, B., Imhof, B. A. and Wehrle-Haller, B. 2001. Marching at the front and dragging behind: Differential alphaVbeta3-integrin turnover regulates focal adhesion behavior. J. Cell Biol. 155, 1319-1332.PubMedGoogle Scholar
  12. Bao, L., Loda, M., Janmey, P. A., Stewart, R., Anand-Apte, B. and Zetter, B. R. 1996. Thymosin beta 15: A novel regulator of tumor cell motility upregulated in metastatic prostate cancer. Nat. Med. 2, 1322-1328.PubMedGoogle Scholar
  13. Bao, L., Loda, M. and Zetter, B. R. 1998. Thymosin beta15 expression in tumor cell lines with varying metastatic potential. Clin. Exp. Metastasis 16, 227-233.PubMedGoogle Scholar
  14. Barzik, M., Kotova, T. I., Higgs, H. N., Hazelwood, L., Hanein, D., Gertler, F. B. and Schafer, D. A. 2005. Ena/VASP proteins enhance actin polymerization in the presence of barbed end capping proteins. J. Biol. Chem. 280, 28653-28662.PubMedGoogle Scholar
  15. Berx, G. and Van Roy, F. 2001. The E-cadherin/catenin complex: An important gatekeeper in breast cancer tumorigenesis and malignant progression. Breast Cancer Res. 3, 289-293.PubMedGoogle Scholar
  16. Blain, E. J., Mason, D. J. and Duance, V. C. 2002. The effect of thymosin beta4 on articular cartilage chondrocyte matrix metalloproteinase expression. Biochem. Soc. Trans. 30, 879-882.PubMedGoogle Scholar
  17. Boccellino, M., Giuberti, G., Quagliuolo, L., Marra, M., D’Alessandro, A. M., Fujita, H., Giovane, A., Abbruzzese, A. and Caraglia, M. 2004. Apoptosis induced by interferon-alpha and antagonized by EGF is regulated by caspase-3-mediated cleavage of gelsolin in human epidermoid cancer cells. J. Cell. Physiol. 201, 71-83.PubMedGoogle Scholar
  18. Bock-Marquette, I., Saxena, A., White, M. D., Dimaio, J. M. and Srivastava, D. 2004. Thymosin beta4 activates integrin-linked kinase and promotes cardiac cell migration, survival and cardiac repair. Nature 432, 466-472.PubMedGoogle Scholar
  19. Bossis, I. and Stratakis, C. A. 2004. PRKAR1A: Normal and abnormal functions. Endocrinology 145, 5452-5458.PubMedGoogle Scholar
  20. Brabletz, T., Jung, A., Spaderna, S., Hlubek, F. and Kirchner, T. 2005. Opinion: Migrating cancer stem cells - An integrated concept of malignant tumour progression. Nat. Rev. Cancer 5, 744-749.PubMedGoogle Scholar
  21. Braverman, R. H., Cooper, H. L., Lee, H. S. and Prasad, G. L. 1996. Anti-oncogenic effects of tropomyosin: Isoform specificity and importance of protein coding sequences. Oncogene 13, 537-545.PubMedGoogle Scholar
  22. Brunton, V. G., MacPherson, I. R. and Frame, M. C. 2004. Cell adhesion receptors, tyrosine kinases and actin modulators: A complex three-way circuitry. Biochim. Biophys. Acta 1692, 121-144.PubMedGoogle Scholar
  23. Bryce, N. S., Clark, E. S., Leysath, J. L., Currie, J. D., Webb, D. J. and Weaver, A. M. 2005. Cortactin promotes cell motility by enhancing lamellipodial persistence. Curr. Biol. 15, 1276-1285.PubMedGoogle Scholar
  24. Buccione, R., Orth, J. D. and McNiven, M. A. 2004. Foot and mouth: Podosomes, invadopodia and circular dorsal ruffles. Nat. Rev. Mol. Cell. Biol. 5, 647-657.PubMedGoogle Scholar
  25. Budhu, A. S., Zipser, B., Forgues, M., Ye, Q. H., Sun, Z. and Wang, X. W. 2005. The molecular signature of metastases of human hepatocellular carcinoma. Oncology 69 (Suppl. 1), 23-27.PubMedGoogle Scholar
  26. Burridge, K. and Wennerberg, K. 2004. Rho and Rac take center stage. Cell 116, 167-179.PubMedGoogle Scholar
  27. Carmeci, C., Thompson, D. A., Kuang, W. W., Lightdale, N., Furthmayr, H. and Weigel, R. J. 1998. Moesin expression is associated with the estrogen receptor-negative breast cancer phenotype. Surgery 124, 211-217.PubMedGoogle Scholar
  28. Carmeliet, P. 2005. VEGF as a key mediator of angiogenesis in cancer. Oncology 69(Suppl. 3), 4-10.PubMedGoogle Scholar
  29. Cha, H. J., Jeong, M. J. and Kleinman, H. K. 2003. Role of thymosin beta4 in tumor metastasis and angiogenesis. J. Natl Cancer Inst. 95, 1674-1680.PubMedGoogle Scholar
  30. Chakravatri, A., Zehr, E. M., Zietman, A. L., Shipley, W. U., Goggins, W. B., Finkelstein, D. M., Young, R. H., Chang, E. L. and Wu, C. L. 2000. Thymosin beta-15 predicts for distant failure in patients with clinically localized prostate cancer - Results from a pilot study. Urology 55, 635-638.PubMedGoogle Scholar
  31. Chandrasekar, I., Stradal, T. E., Holt, M. R., Entschladen, F., Jockusch, B. M. and Ziegler, W. H. 2005. Vinculin acts as a sensor in lipid regulation of adhesion-site turnover. J. Cell Sci. 118, 1461-1472.PubMedGoogle Scholar
  32. Chaponnier, C. and Gabbiani, G. 1989. Gelsolin modulation in epithelial and stromal cells of mammary carcinoma. Am. J. Pathol. 134, 597-603.PubMedGoogle Scholar
  33. Chaponnier, C. and Gabbiani, G. 2004. Pathological situations characterized by altered actin isoform expression. J. Pathol. 204, 386-395.PubMedGoogle Scholar
  34. Chiappetta, G., Pentimalli, F., Monaco, M., Fedele, M., Pasquinelli, R., Pierantoni, G. M., Ribecco, M. T., Santelli, G., Califano, D., Pezzullo, L. and Fusco, A. 2004. Thymosin beta-10 gene expression as a possible tool in diagnosis of thyroid neoplasias. Oncol. Rep. 12, 239-243.PubMedGoogle Scholar
  35. Chitaley, K., Chen, L., Galler, A., Walter, U., Daum, G. and Clowes, A. W. 2004. Vasodilator-stimulated phosphoprotein is a substrate for protein kinase C. FEBS Lett. 556, 211-215.PubMedGoogle Scholar
  36. Chu, Y. S., Thomas, W. A., Eder, O., Pincet, F., Perez, E., Thiery, J. P. and Dufour, S. 2004. Force measurements in E-cadherin-mediated cell doublets reveal rapid adhesion strengthened by actin cytoskeleton remodeling through Rac and Cdc42. J. Cell Biol. 167, 1183-1194.PubMedGoogle Scholar
  37. Chuma, M., Sakamoto, M., Yasuda, J., Fujii, G., Nakanishi, K., Tsuchiya, A., Ohta, T., Asaka, M. and Hirohashi, S. 2004. Overexpression of cortactin is involved in motility and metastasis of hepatocellular carcinoma. J. Hepatol. 41, 629-636.PubMedGoogle Scholar
  38. Clark, E. A., Golub, T. R., Lander, E. S. and Hynes, R. O. 2000. Genomic analysis of metastasis reveals an essential role for RhoC. Nature 406, 532-535.PubMedGoogle Scholar
  39. Coello, M. C., Luketich, J. D., Litle, V. R. and Godfrey, T. E. 2004. Prognostic significance of micrometastasis in non-small-cell lung cancer. Clin. Lung Cancer 5, 214-225.PubMedGoogle Scholar
  40. Condeelis, J. 1993. Understanding the cortex of crawling cells: Insights from Dictyostelium. Trends Cell Biol. 3, 371-376.PubMedGoogle Scholar
  41. Condeelis, J. and Segall, J. E. 2003. Intravital imaging of cell movement in tumours. Nat. Rev. Cancer 3, 921-930.PubMedGoogle Scholar
  42. Condeelis, J., Singer, R. H. and Segall, J. E. 2005. The great escape: When cancer cells hijack the genes for chemotaxis and motility. Annu. Rev. Cell Dev. Biol. 21, 695-718.PubMedGoogle Scholar
  43. Critchley, D. R. 2004. Cytoskeletal proteins talin and vinculin in integrin-mediated adhesion. Biochem. Soc. Trans. 32, 831-836.PubMedGoogle Scholar
  44. Cunningham, C. C., Stossel, T. P. and Kwiatkowski, D. J. 1991. Enhanced motility in NIH 3T3 fibroblasts that overexpress gelsolin. Science 251, 1233-1236.PubMedGoogle Scholar
  45. Curto, M. and McClatchey, A. I. 2004. Ezrin, a metastatic detERMinant? Cancer Cell 5,113-114.PubMedGoogle Scholar
  46. Daly, R. J. 2004. Cortactin signalling and dynamic actin networks. Biochem. J. 382, 13-25.PubMedGoogle Scholar
  47. Dang, D., Bamburg, J. R. and Ramos, D. M. 2006. Alphavbeta3 integrin and cofilin modulate K1735 melanoma cell invasion. Exp. Cell Res. 312, 468-477.PubMedGoogle Scholar
  48. Davila, M., Frost, A. R., Grizzle, W. E. and Chakrabarti, R. 2003. LIM kinase 1 is essential for the invasive growth of prostate epithelial cells: Implications in prostate cancer. J. Biol. Chem. 278, 36868-36875.PubMedGoogle Scholar
  49. De Corte, V., Bruyneel, E., Boucherie, C., Mareel, M., Vandekerckhove, J. and Gettemans, J. 2002. Gelsolin-induced epithelial cell invasion is dependent on Ras-Rac signaling. EMBO J. 21, 6781-6790.PubMedGoogle Scholar
  50. De Corte, V., Gettemans, J. and Vandekerckhove, J. 1997. Phosphatidylinositol 4, 5-bisphosphate specifically stimulates PP60(c-src) catalyzed phosphorylation of gelsolin and related actin-binding proteins. FEBS Lett. 401, 191-196.PubMedGoogle Scholar
  51. De Corte, V., Van Impe, K., Bruyneel, E., Boucherie, C., Mareel, M., Vandekerckhove, J. and Gettemans, J. 2004. Increased importin-beta-dependent nuclear import of the actin modulating protein CapG promotes cell invasion. J. Cell Sci. 117, 5283-5292.PubMedGoogle Scholar
  52. De Craene, B., Gilbert, B., Stove, C., Bruyneel, E., van Roy, F. and Berx, G. 2005. The transcription factor snail induces tumor cell invasion through modulation of the epithelial cell differentiation program. Cancer Res. 65, 6237-6244.PubMedGoogle Scholar
  53. De Ganck, A., Hubert, T., Van Impe, K., Geelen, D., Vandekerckhove, J., De Corte, V. and Gettemans, J. 2005. A monopartite nuclear localization sequence regulates nuclear targeting of the actin binding protein myopodin. FEBS Lett. 579, 6673-6680.PubMedGoogle Scholar
  54. De Wever, O., Nguyen, Q. D., Van Hoorde, L., Bracke, M., Bruyneel, E., Gespach, C. and Mareel, M. 2004a. Tenascin-C and SF/HGF produced by myofibroblasts in vitro provide convergent pro-invasive signals to human colon cancer cells through RhoA and Rac. FASEB J. 18, 1016-1018.Google Scholar
  55. De Wever, O., Westbroek, W., Verloes, A., Bloemen, N., Bracke, M., Gespach, C., Bruyneel, E. and Mareel, M. 2004b. Critical role of N-cadherin in myofibroblast invasion and migration in vitro stimulated by colon-cancer-cell-derived TGF-beta or wounding. J. Cell Sci. 117, 4691-4703.Google Scholar
  56. Delanote, V., Vandekerckhove, J. and Gettemans, J. 2005. Plastins: Versatile modulators of actin organization in (patho)physiological cellular processes. Acta Pharmacol. Sin. 26, 769-779.PubMedGoogle Scholar
  57. Dertsiz, L., Ozbilim, G., Kayisli, Y., Gokhan, G. A., Demircan, A. and Kayisli, U. A. 2005. Differential expression of VASP in normal lung tissue and lung adenocarcinomas. Thorax 60, 576-581.PubMedGoogle Scholar
  58. DesMarais, V., Ghosh, M., Eddy, R. and Condeelis, J. 2005. Cofilin takes the lead. J. Cell Sci. 118, 19-26.PubMedGoogle Scholar
  59. DesMarais, V., Macaluso, F., Condeelis, J. and Bailly, M. 2004. Synergistic interaction between the Arp2/3 complex and cofilin drives stimulated lamellipod extension. J. Cell Sci. 117, 3499-3510.PubMedGoogle Scholar
  60. Dhanasekaran, S. M., Barrette, T. R., Ghosh, D., Shah, R., Varambally, S., Kurachi, K., Pienta, K. J., Rubin, M. A. and Chinnaiyan, A. M. 2001. Delineation of prognostic biomarkers in prostate cancer. Nature 412, 822-826.PubMedGoogle Scholar
  61. Di Modugno, F., Bronzi, G., Scanlan, M. J., Del Bello, D., Cascioli, S., Venturo, I., Botti, C., Nicotra, M. R., Mottolese, M., Natali, P. G., Santoni, A., Jager, E. and Nistico, P. 2004. Human Mena protein, a serex-defined antigen overexpressed in breast cancer eliciting both humoral and CD8+ T-cell immune response. Int. J. Cancer 109, 909-918.PubMedGoogle Scholar
  62. Ding, S. J., Li, Y., Shao, X. X., Zhou, H., Zeng, R., Tang, Z. Y. and Xia, Q. C. 2004. Proteome analysis of hepatocellular carcinoma cell strains, MHCC97-H and MHCC97-L, with different metastasis potentials. Proteomics 4, 982-994.PubMedGoogle Scholar
  63. Dong, Y., Asch, H. L., Ying, A. and Asch, B. B. 2002. Molecular mechanism of transcriptional repression of gelsolin in human breast cancer cells. Exp. Cell Res. 276, 328-336.PubMedGoogle Scholar
  64. Dosaka-Akita, H., Hommura, F., Fujita, H., Kinoshita, I., Nishi, M., Morikawa, T., Katoh, H., Kawakami, Y. and Kuzumak, N. 1998. Frequent loss of gelsolin expression in non-small cell lung cancers of heavy smokers. Cancer Res. 58, 322-327.PubMedGoogle Scholar
  65. Edwards, D. C., Sanders, L. C., Bokoch, G. M. and Gill, G. N. 1999. Activation of LIM-kinase by Pak1 couples Rac/Cdc42 GTPase signalling to actin cytoskeletal dynamics. Nat. Cell Biol. 1, 253-259.PubMedGoogle Scholar
  66. Elliott, B. E., Meens, J. A., SenGupta, S. K., Louvard, D. and Arpin, M. 2005. The membrane cytoskeletal crosslinker ezrin is required for metastasis of breast carcinoma cells. Breast Cancer Res. 7, 365-373.Google Scholar
  67. Everley, P. A., Krijgsveld, J., Zetter, B. R. and Gygi, S. P. 2004. Quantitative cancer proteomics: Stable isotope labeling with amino acids in cell culture (SILAC) as a tool for prostate cancer research. Mol. Cell Proteomics 3, 729-735.PubMedGoogle Scholar
  68. Faix, J. and Rottner, K. 2006. The making of filopodia. Curr. Opin. Cell Biol. 18, 18-25.PubMedGoogle Scholar
  69. Falet, H., Hoffmeister, K. M., Neujahr, R., Italiano, J. E., Jr., Stossel, T. P., Southwick, F. S. and Hartwig, J. H. 2002. Importance of free actin filament barbed ends for Arp2/3 complex function in platelets and fibroblasts. Proc. Natl Acad. Sci. USA 99, 16782-16787.PubMedGoogle Scholar
  70. Farina, K. L., Wyckoff, J. B., Rivera, J., Lee, H., Segall, J. E., Condeelis, J. S. and Jones, J. G. 1998. Cell motility of tumor cells visualized in living intact primary tumors using green fluorescent protein. Cancer Res. 58, 2528-2532.PubMedGoogle Scholar
  71. Ferlay, J., Bray, F., Pisani, P. and Parkin, D. M. 2004. GLOBOCAN 2002: Cancer Incidence, Mortality and Prevalence Worldwide. IARCPress, Lyon.Google Scholar
  72. Flanagan, L. A., Chou, J., Falet, H., Neujahr, R., Hartwig, J. H. and Stossel, T. P. 2001. Filamin A, the Arp2/3 complex and the morphology and function of cortical actin filaments in human melanoma cells. J. Cell Biol. 155, 511-517.PubMedGoogle Scholar
  73. Franca-Koh, J. and Devreotes, P. N. 2004. Moving forward: Mechanisms of chemoattractant gradient sensing. Physiology 19, 300-308.PubMedGoogle Scholar
  74. Franco, S. J. and Huttenlocher, A. 2005. Regulating cell migration: Calpains make the cut. J. Cell Sci. 118, 3829-3838.PubMedGoogle Scholar
  75. Friedl, P. 2004. Prespecification and plasticity: Shifting mechanisms of cell migration. Curr. Opin. Cell Biol. 16, 14-23.PubMedGoogle Scholar
  76. Friedl, P., Borgmann, S. and Brocker, E. B. 2001. Amoeboid leukocyte crawling through extracellular matrix: Lessons from the Dictyostelium paradigm of cell movement. J. Leukoc. Biol. 70, 491-509.PubMedGoogle Scholar
  77. Friedl, P. and Brocker, E. B. 2000. The biology of cell locomotion within threedimensional extracellular matrix. Cell. Mol. Life Sci. 57, 41-64.PubMedGoogle Scholar
  78. Friedl, P., Hegerfeldt, Y. and Tusch, M. 2004. Collective cell migration in morphogenesis and cancer. Int. J. Dev. Biol. 48, 441-449.PubMedGoogle Scholar
  79. Friedl, P., Maaser, K., Klein, C. E., Niggemann, B., Krohne, G. and Zanker, K. S. 1997. Migration of highly aggressive MV3 melanoma cells in 3-dimensional collagen lattices results in local matrix reorganization and shedding of alpha2 and beta1 integrins and CD44. Cancer Res. 57, 2061-2070.PubMedGoogle Scholar
  80. Friedl, P. and Wolf, K. 2003. Proteolytic and non-proteolytic migration of tumour cells and leucocytes. Biochem. Soc. Symp. 70, 277-285.PubMedGoogle Scholar
  81. Fujita, H., Okada, F., Hamada, J., Hosokawa, M., Moriuchi, T., Koya, R. C. and Kuzumaki, N. 2001. Gelsolin functions as a metastasis suppressor in B16-BL6 mouse melanoma cells and requirement of the carboxyl-terminus for its effect. Int. J. Cancer 93, 773-780.PubMedGoogle Scholar
  82. Futreal, P. A., Coin, L., Marshall, M., Down, T., Hubbard, T., Wooster, R., Rahman, N. and Stratton, M. R. 2004. A census of human cancer genes. Nat. Rev. Cancer 4, 177-183.PubMedGoogle Scholar
  83. Galiegue-Zouitina, S., Quief, S., Hildebrand, M. P., Denis, C., Detourmignies, L., Lai, J. L. and Kerckaert, J. P. 1999. Nonrandom fusion of L-plastin(LCP1) and LAZ3(BCL6) genes by t(3;13)(q27;q14) chromosome translocation in two cases of B-cell non-Hodgkin lymphoma. Genes Chromosomes Cancer 26, 97-105.PubMedGoogle Scholar
  84. Gamba, A., de Candia, A., Di Talia, S., Coniglio, A., Bussolino, F. and Serini, G. 2005. Diffusion-limited phase separation in eukaryotic chemotaxis. Proc. Natl Acad. Sci. USA 102, 16927-16932.PubMedGoogle Scholar
  85. Gautreau, A., Manent, J., Fievet, B., Louvard, D., Giovannini, M. and Arpin, M. 2002. Mutant products of the NF2 tumor suppressor gene are degraded by the ubiquitin-proteasome pathway. J. Biol. Chem. 277, 31279-31282.PubMedGoogle Scholar
  86. Gettemans, J., Van Impe, K., Delanote, V., Hubert, T., Vandekerckhove, J. and De Corte, V. 2005. Nuclear actin-binding proteins as modulators of gene transcription. Traffic 6, 847-857.PubMedGoogle Scholar
  87. Giganti, A., Plastino, J., Janji, B., Van Troys, M., Lentz, D., Ampe, C., Sykes, C., and Friederich, E. 2005. Actin-filament cross-linking protein T-plastin increases Arp2/3-mediated actin-based movement. J. Cell Sci. 118, 1255-1265.PubMedGoogle Scholar
  88. Gimona, M., Kazzaz, J. A. and Helfman, D. M. 1996. Forced expression of tropomyosin 2 or 3 in v-Ki-ras-transformed fibroblasts results in distinct phenotypic effects. Proc. Natl Acad. Sci. USA 93, 9618-9623.PubMedGoogle Scholar
  89. Glukhova, M., Koteliansky, V., Sastre, X. and Thiery, J. P. 1995. Adhesion systems in normal breast and in invasive breast carcinoma. Am. J. Pathol. 146, 706-716.PubMedGoogle Scholar
  90. Gotoh, A. and Broxmeyer, H. E. 1997. The function of BCR/ABL and related protooncogenes. Curr. Opin. Hematol. 4, 3-11.PubMedCrossRefGoogle Scholar
  91. Gronborg, M., Kristiansen, T. Z., Iwahori, A., Chang, R., Reddy, R., Sato, N., Molina, H., Jensen, O. N., Hruban, R. H., Goggins, M. G., Maitra, A. and Pandey, A. 2006. Biomarker discovery from pancreatic cancer secretome using a differential proteomic approach. Mol. Cell. Proteomics 5, 157-171.PubMedGoogle Scholar
  92. Grosse, R., Copeland, J. W., Newsome, T. P., Way, M. and Treisman, R. 2003. A role for VASP in RhoA-Diaphanous signalling to actin dynamics and SRF activity. EMBO J. 22, 3050-3061.PubMedGoogle Scholar
  93. Gunnersen, J. M., Spirkoska, V., Smith, P. E., Danks, R. A. and Tan, S. S. 2000. Growth and migration markers of rat C6 glioma cells identified by serial analysis of gene expression. Glia 32, 146-154.PubMedGoogle Scholar
  94. Gunning, P. W., Schevzov, G., Kee, A. J. and Hardeman, E. C. 2005. Tropomyosin isoforms: Divining rods for actin cytoskeleton function. Trends Cell Biol. 15, 333-341.PubMedGoogle Scholar
  95. Gupton, S. L., Anderson, K. L., Kole, T. P., Fischer, R. S., Ponti, A., Hitchcock-DeGregori, S. E., Danuser, G., Fowler, V. M., Wirtz, D., Hanein, D. and WatermanStorer, C. M. 2005. Cell migration without a lamellipodium: Translation of actin dynamics into cell movement mediated by tropomyosin. J. Cell Biol. 168, 619-631.PubMedGoogle Scholar
  96. Gutmann, D. H. 2001. The neurofibromatoses: When less is more. Hum. Mol. Genet. 10,747-755.PubMedGoogle Scholar
  97. Hall, A. K. 1994. Amplification-independent overexpression of thymosin beta-10 mRNA in human renal cell carcinoma. Ren. Fail. 16, 243-254.PubMedGoogle Scholar
  98. Hanahan, D. and Weinberg, R. A. 2000. The hallmarks of cancer. Cell 100, 57-70.PubMedGoogle Scholar
  99. Hashimoto, Y., Skacel, M. and Adams, J. C. 2005. Roles of fascin in human carcinoma motility and signaling: Prospects for a novel biomarker? Int. J. Biochem. Cell Biol. 37, 1787-1804.PubMedGoogle Scholar
  100. Hegerfeldt, Y., Tusch, M., Brocker, E. B. and Friedl, P. 2002. Collective cell movement in primary melanoma explants: Plasticity of cell-cell interaction, beta1-integrin function and migration strategies. Cancer Res. 62, 2125-2130.PubMedGoogle Scholar
  101. Hernandez, S. E., Krishnaswami, M., Miller, A. L. and Koleske, A. J. 2004. How do Abl family kinases regulate cell shape and movement? Trends Cell Biol. 14, 36-44.PubMedGoogle Scholar
  102. Hilpela, P., Vartiainen, M. K. and Lappalainen, P. 2004. Regulation of the actin cytoskeleton by PI(4,5)P2 and PI(3,4,5)P3. Curr. Top. Microbiol. Immunol. 282, 117-163.PubMedGoogle Scholar
  103. Hoffman, R. M. 2005. In vivo cell biology of cancer cells visualized with fluorescent proteins. Curr. Top. Dev. Biol. 70, 121-144.PubMedGoogle Scholar
  104. Honda, K., Yamada, T., Endo, R., Ino, Y., Gotoh, M., Tsuda, H., Yamada, Y., Chiba, H. and Hirohashi, S. 1998. Actinin-4, a novel actin-bundling protein associated with cell motility and cancer invasion. J. Cell Biol. 140, 1383-1393.PubMedGoogle Scholar
  105. Honda, K., Yamada, T., Hayashida, Y., Idogawa, M., Sato, S., Hasegawa, F., Ino, Y., Ono, M. and Hirohashi, S. 2005. Actinin-4 increases cell motility and promotes lymph node metastasis of colorectal cancer. Gastroenterology 128, 51-62.PubMedGoogle Scholar
  106. Hou, Y., Gupta, N., Shoenlein, P., Wong, E., Martindale, R., Ganapathy, V. and Browning, D. 2006. An anti-tumor role for cGMP-dependent protein kinase. Cancer Lett. 240, 60-68.PubMedGoogle Scholar
  107. Huber, M. A., Kraut, N. and Beug, H. 2005. Molecular requirements for epithelial-mesenchymal transition during tumor progression. Curr. Opin. Cell Biol. 17,548-558.PubMedGoogle Scholar
  108. Huff, T., Muller, C. S., Otto, A. M., Netzker, R. and Hannappel, E. 2001. beta-thymosins, small acidic peptides with multiple functions. Int. J. Biochem. Cell Biol. 33, 205-220.PubMedGoogle Scholar
  109. Huff, T., Rosorius, O., Otto, A. M., Muller, C. S., Ballweber, E., Hannappel, E. and Mannherz, H. G. 2004. Nuclear localisation of the G-actin sequestering peptide thymosin beta4. J. Cell Sci. 117, 5333-5341.PubMedGoogle Scholar
  110. Hunter, K. W. 2004. Ezrin, a key component in tumor metastasis. Trends Mol. Med. 10,201-204.PubMedGoogle Scholar
  111. Hutchinson, L. M., Chang, E. L., Becker, C. M., Shih, M. C., Brice, M., DeWolf, W. C., Gaston, S. M. and Zetter, B. R. 2005. Use of thymosin beta15 as a urinary biomarker in human prostate cancer. Prostate 64, 116-127.PubMedGoogle Scholar
  112. Huttelmaier, S., Zenklusen, D., Lederer, M., Dictenberg, J., Lorenz, M., Meng, X., Bassell, G. J., Condeelis, J. and Singer, R. H. 2005. Spatial regulation of beta-actin translation by Src-dependent phosphorylation of ZBP1. Nature 438, 512-515.PubMedGoogle Scholar
  113. Huttenlocher, A., Ginsberg, M. H. and Horwitz, A. F. 1996. Modulation of cell migration by integrin-mediated cytoskeletal linkages and ligand-binding affinity. J. Cell Biol. 134, 1551-1562.PubMedGoogle Scholar
  114. Islam, A. H., Ehara, T., Kato, H., Hayama, M. and Nishizawa, O. 2004. Loss of calponin h1 in renal angiomyolipoma correlates with aggressive clinical behavior. Urology 64, 468-473.PubMedGoogle Scholar
  115. Janke, J., Schluter, K., Jandrig, B., Theile, M., Kolble, K., Arnold, W., Grinstein, E., Schwartz, A., Estevez-Schwarz, L., Schlag, P. M., Jockusch, B. M. and Scherneck, S. 2000. Suppression of tumorigenicity in breast cancer cells by the microfilament protein profilin 1. J. Exp. Med. 191, 1675-1686.PubMedGoogle Scholar
  116. Jawhari, A. U., Buda, A., Jenkins, M., Shehzad, K., Sarraf, C., Noda, M., Farthing, M. J., Pignatelli, M. and Adams, J. C. 2003. Fascin, an actin-bundling protein, modulates colonic epithelial cell invasiveness and differentiation in vitro. Am. J. Pathol. 162, 69-80.PubMedGoogle Scholar
  117. Jing, L., Liu, L., Yu, Y. P., Dhir, R., Acquafondada, M., Landsittel, D., Cieply, K., Wells, A. and Luo, J. H. 2004. Expression of myopodin induces suppression of tumor growth and metastasis. Am. J. Pathol. 164, 1799-1806.PubMedGoogle Scholar
  118. Kabukcuoglu, S., Ozalp, S. S., Oner, U., Acikalin, M. F., Yalcin, O. T. and Colak, E. 2005. Fascin, an actin-bundling protein expression in cervical neoplasms. Eur. J. Gynaecol. Oncol. 26, 636-641.PubMedGoogle Scholar
  119. Kaneda, A., Kaminishi, M., Sugimura, T. and Ushijima, T. 2004. Decreased expression of the seven ARP2/3 complex genes in human gastric cancers. Cancer Lett. 212, 203-210.PubMedGoogle Scholar
  120. Kaneda, A., Kaminishi, M., Yanagihara, K., Sugimura, T. and Ushijima, T. 2002. Identification of silencing of nine genes in human gastric cancers. Cancer Res. 62, 6645-6650.PubMedGoogle Scholar
  121. Kanehisa, M., Goto, S., Hattori, M., Aoki-Kinoshita, K. F., Itoh, M., Kawashima, S., Katayama, T., Araki, M. and Hirakawa, M. 2006. From genomics to chemical genomics: New developments in KEGG. Nucleic Acids Res. 34, 354-357.Google Scholar
  122. Kassis, J., Moellinger, J., Lo, H., Greenberg, N. M., Kim, H. G. and Wells, A. 1999. A role for phospholipase C-gamma-mediated signaling in tumor cell invasion. Clin. Cancer Res. 5, 2251-2260.PubMedGoogle Scholar
  123. Kaverina, I., Krylyshkina, O. and Small, J. V. 2002. Regulation of substrate adhesion dynamics during cell motility. Int. J. Biochem. Cell Biol. 34, 746-761.PubMedGoogle Scholar
  124. Keller, R. 2005. Cell migration during gastrulation. Curr. Opin. Cell Biol. 17, 533-541.PubMedGoogle Scholar
  125. Khanna, C., Wan, X., Bose, S., Cassaday, R., Olomu, O., Mendoza, A., Yeung, C., Gorlick, R., Hewitt, S. M. and Helman, L. J. 2004. The membrane-cytoskeleton linker ezrin is necessary for osteosarcoma metastasis. Nat. Med. 10, 182-186.PubMedGoogle Scholar
  126. Klein, C. A. 2004. Gene expression sigantures, cancer cell evolution and metastatic progression. Cell Cycle 3, 29-31.PubMedGoogle Scholar
  127. Kobayashi, T., Okada, F., Fujii, N., Tomita, N., Ito, S., Tazawa, H., Aoyama, T., Choi, S. K., Shibata, T., Fujita, H. and Hosokawa, M. 2002. Thymosin-beta4 regulates motility and metastasis of malignant mouse fibrosarcoma cells. Am. J. Pathol. 160, 869-882.PubMedGoogle Scholar
  128. Koon, N., Schneider-Stock, R., Sarlomo-Rikala, M., Lasota, J., Smolkin, M., Petroni, G., Zaika, A., Boltze, C., Meyer, F., Andersson, L., Knuutila, S., Miettinen, M. and El-Rifai, W. 2004. Molecular targets for tumour progression in gastrointestinal stromal tumours. Gut 53, 235-240.PubMedGoogle Scholar
  129. Kovar, D. R., Wu, J. Q. and Pollard, T. D. 2005. Profilin-mediated competition between capping protein and formin Cdc12p during cytokinesis in fission yeast. Mol. Biol. Cell 16, 2313-2324.PubMedGoogle Scholar
  130. Kurisu, S., Suetsugu, S., Yamazaki, D., Yamaguchi, H. and Takenawa, T. 2005. RacWAVE2 signaling is involved in the invasive and metastatic phenotypes of murine melanoma cells. Oncogene 24, 1309-1319.PubMedGoogle Scholar
  131. Lal, A., Lash, A. E., Altschul, S. F., Velculescu, V., Zhang, L., McLendon, R. E., Marra, M. A., Prange, C., Morin, P. J., Polyak, K., Papadopoulos, N., Vogelstein, B., Kinzler, K. W., Strausberg, R. L. and Riggins, G. J. 1999. A public database for gene expression in human cancers. Cancer Res. 59, 5403-5407.PubMedGoogle Scholar
  132. Lambrechts, A., Kwiatkowski, A. V., Lanier, L. M., Bear, J. E., Vandekerckhove, J., Ampe, C. and Gertler, F. B. 2000. cAMP-dependent protein kinase phosphorylation of EVL, a Mena/VASP relative, regulates its interaction with actin and SH3 domains. J. Biol. Chem. 275, 36143-36151.PubMedGoogle Scholar
  133. Lambrechts, A., Van Troys, M. and Ampe, C. 2004. The actin cytoskeleton in normal and pathological cell motility. Int. J. Biochem. Cell Biol. 6, 1890-1909.Google Scholar
  134. Langbein, S., Lehmann, J., Harder, A., Steidler, A., Michel, M. S., Alken, P. and Badawi, J. K. 2006. Protein profiling of bladder cancer using the 2D-PAGE and SELDI-TOF-MS technique. Technol. Cancer Res. Treat. 5, 67-72.PubMedGoogle Scholar
  135. Lapointe, J., Li, C., Higgins, J. P., van de Rijn, M., Bair, E., Montgomery, K., Ferrari, M., Egevad, L., Rayford, W., Bergerheim, U., Ekman, P., DeMarzo, A. M., Tibshirani, R., Botstein, D., Brown, P. O., Brooks, J. D. and Pollack, J. R. 2004. Gene expression profiling identifies clinically relevant subtypes of prostate cancer. Proc. Natl Acad. Sci. USA 101, 811-816.PubMedGoogle Scholar
  136. LaTulippe, E., Satagopan, J., Smith, A., Scher, H., Scardino, P., Reuter, V. and Gerald, W. L. 2002. Comprehensive gene expression analysis of prostate cancer reveals distinct transcriptional programs associated with metastatic disease. Cancer Res. 62, 4499-4506.PubMedGoogle Scholar
  137. Lauffenburger, D. A. and Horwitz, A. F. 1996. Cell migration: A physically integrated molecular process. Cell 84, 359-369.PubMedGoogle Scholar
  138. Lawrence, B., Perez-Atayde, A., Hibbard, M. K., Rubin, B. P., Dal, C. P., Pinkus, J. L., Pinkus, G. S., Xiao, S., Yi, E., Fletcher, C. D. and Fletcher, J. A. 2000. TPM3-ALK and TPM4-ALK oncogenes in inflammatory myofibroblastic tumors. Am. J. Pathol. 157, 377-384.PubMedGoogle Scholar
  139. Leavitt, J., Ng, S. Y., Varma, M., Latter, G., Burbeck, S., Gunning, P. and Kedes, L. 1987. Expression of transfected mutant beta-actin genes: Transitions toward the stable tumorigenic state. Mol. Cell. Biol. 7, 2467-2476.PubMedGoogle Scholar
  140. Lee, H. K., Driscoll, D., Asch, H., Asch, B. and Zhang, P. J. 1999. Downregulated gelsolin expression in hyperplastic and neoplastic lesions of the prostate. Prostate 40,14-19.PubMedGoogle Scholar
  141. Lee, Y. G., Macoska, J. A., Korenchuk, S. and Pienta, K. J. 2002. MIM, a potential metastasis suppressor gene in bladder cancer. Neoplasia 4, 291-294.PubMedGoogle Scholar
  142. Lee, Y. J., Mazzatti, D. J., Yun, Z. and Keng, P. C. 2005b. Inhibition of invasiveness of human lung cancer cell line H1299 by over-expression of cofilin. Cell Biol. Int. 29,877-883.Google Scholar
  143. Lee, S. H., Son, M. J., Oh, S. H., Rho, S. B., Park, K., Kim, Y. J., Park, M. S. and Lee, J. H. 2005a. Thymosin {beta}(10) inhibits angiogenesis and tumor growth by interfering with Ras function. Cancer Res. 65, 137-148.Google Scholar
  144. Lee, S. H., Zhang, W., Choi, J. J., Cho, Y. S., Oh, S. H., Kim, J. W., Hu, L., Xu, J., Liu, J. and Lee, J. H. 2001. Overexpression of the thymosin beta-10 gene in human ovarian cancer cells disrupts F-actin stress fiber and leads to apoptosis. Oncogene 20, 6700-6706.PubMedGoogle Scholar
  145. Lener, T., Burgstaller, G. and Gimona, M. 2004. The role of calponin in the gene profile of metastatic cells: Inhibition of metastatic cell motility by multiple calponin repeats. FEBS Lett. 556, 221-226.PubMedGoogle Scholar
  146. Li, Y., Tondravi, M., Liu, J., Smith, E., Haudenschild, C. C., Kaczmarek, M. and Zhan, X. 2001. Cortactin potentiates bone metastasis of breast cancer cells. Cancer Res. 61, 6906-6911.PubMedGoogle Scholar
  147. Lin, C. S., Park, T., Chen, Z. P. and Leavitt, J. 1993. Human plastin genes. Comparative gene structure, chromosome location and differential expression in normal and neoplastic cells. J. Biol. Chem. 268, 2781-2792.PubMedGoogle Scholar
  148. Lin, Y. H., Park, Z. Y., Lin, D., Brahmbhatt, A. A., Rio, M. C., Yates, J. R. and Klemke, R. L. 2004. Regulation of cell migration and survival by focal adhesion targeting of Lasp-1. J. Cell Biol. 165, 421-432.PubMedGoogle Scholar
  149. Lin, F., Yu, Y. P., Woods, J., Cieply, K., Gooding, B., Finkelstein, P., Dhir, R., Krill, D., Becich, M. J., Michalopoulos, G., Finkelstein, S. and Luo, J. H. 2001. Myopodin, a synaptopodin homologue, is frequently deleted in invasive prostate cancers. Am. J. Pathol. 159, 1603-1612.PubMedGoogle Scholar
  150. Linder, S. and Kopp, P. 2005. Podosomes at a glance. J. Cell Sci. 118, 2079-2082.PubMedGoogle Scholar
  151. Loberg, R. D., Neeley, C. K., Adam-Day, L. L., Fridman, Y., St John, L. N., Nixdorf, S., Jackson, P., Kalikin, L. M. and Pienta, K. J. 2005. Differential expression analysis of MIM (MTSS1) splice variants and a functional role of MIM in prostate cancer cell biology. Int. J. Oncol. 26, 1699-1705.PubMedGoogle Scholar
  152. Lorenz, M., Yamaguchi, H., Wang, Y., Singer, R. H. and Condeelis, J. 2004. Imaging sites of N-wasp activity in lamellipodia and invadopodia of carcinoma cells. Curr. Biol. 14, 697-703.PubMedGoogle Scholar
  153. Lukasik, S. M., Zhang, L., Corpora, T., Tomanicek, S., Li, Y., Kundu, M., Hartman, K., Liu, P. P., Laue, T. M., Biltonen, R. L., Speck, N. A. and Bushweller, J. H. 2002. Altered affinity of CBF beta-SMMHC for Runx1 explains its role in leukemogenesis. Nat. Struct. Biol. 9, 674-679.PubMedGoogle Scholar
  154. Luo, J. H., Yu, Y. P., Cieply, K., Lin, F., Deflavia, P., Dhir, R., Finkelstein, S., Michalopoulos, G. and Becich, M. 2002. Gene expression analysis of prostate cancers. Mol. Carcinog. 33, 25-35.PubMedGoogle Scholar
  155. Maaser, K., Wolf, K., Klein, C. E., Niggemann, B., Zanker, K. S., Brocker, E. B. and Friedl, P. 1999. Functional hierarchy of simultaneously expressed adhesion receptors: Integrin alpha2beta1 but not CD44 mediates MV3 melanoma cell migration and matrix reorganization within three-dimensional hyaluronan-containing collagen matrices. Mol. Biol. Cell 10, 3067-3079.PubMedGoogle Scholar
  156. Machesky, L. M., Atkinson, S. J., Ampe, C., Vandekerckhove, J. and Pollard, T. D. 1994. Purification of a cortical complex containing two unconventional actins from Acanthamoeba by affinity chromatography on profilin-agarose. J. Cell Biol. 127, 107-115.PubMedGoogle Scholar
  157. Mahadev, K., Raval, G., Bharadwaj, S., Willingham, M. C., Lange, E. M., Vonderhaar, B., Salomon, D. and Prasad, G. L. 2002. Suppression of the transformed phenotype of breast cancer by tropomyosin-1. Exp. Cell Res. 279, 40-51.PubMedGoogle Scholar
  158. Mareel, M. and Leroy, A. 2003. Clinical, cellular, and molecular aspects of cancer invasion. Physiol. Rev. 83, 337-376.PubMedGoogle Scholar
  159. Martoglio, A. M., Tom, B. D., Starkey, M., Corps, A. N., Charnock-Jones, D. S. and Smith, S. K. 2000. Changes in tumorigenesis- and angiogenesis-related gene transcript abundance profiles in ovarian cancer detected by tailored high density cDNA arrays. Mol. Med. 6, 750-765.PubMedGoogle Scholar
  160. Maul, R. S., Song, Y., Amann, K. J., Gerbin, S. C., Pollard, T. D. and Chang, D. D. 2003. EPLIN regulates actin dynamics by cross-linking and stabilizing filaments. J. Cell Biol. 160, 399-407.PubMedGoogle Scholar
  161. McClatchey, A. I. and Giovannini, M. 2005. Membrane organization and tumorigenesis - The NF2 tumor suppressor, Merlin. Genes Dev. 19, 2265-2277.Google Scholar
  162. McCormack, S. A., Ray, R. M., Blanner, P. M. and Johnson, L. R. 1999. Polyamine depletion alters the relationship of F-actin, G-actin and thymosin beta4 in migrating IEC-6 cells. Am. J. Physiol. 276, 459-468.Google Scholar
  163. McNiven, M. A., Baldassarre, M. and Buccione, R. 2004. The role of dynamin in the assembly and function of podosomes and invadopodia. Front. Biosci. 9, 1944-1953.PubMedGoogle Scholar
  164. Meyer, T. and Brinck, U. 1997. Immunohistochemical detection of vinculin in human rhabdomyosarcomas. Gen. Diagn. Pathol. 142, 191-198.PubMedGoogle Scholar
  165. Mielnicki, L. M., Ying, A. M., Head, K. L., Asch, H. L. and Asch, B. B. 1999. Epigenetic regulation of gelsolin expression in human breast cancer cells. Exp. Cell Res. 249, 161-176.PubMedGoogle Scholar
  166. Mitchison, T. J. and Cramer, L. P. 1996. Actin-based cell motility and cell locomotion. Cell 84, 371-379.PubMedGoogle Scholar
  167. Mueller, M. M. and Fusenig, N. E. 2004. Friends or foes - Bipolar effects of the tumour stroma in cancer. Nat. Rev. Cancer 4, 839-849.PubMedGoogle Scholar
  168. Muller-Tidow, C., Diederichs, S., Thomas, M. and Serve, H. 2004. Genome-wide screening for prognosis-predicting genes in early-stage non-small-cell lung cancer. Lung Cancer 45, S145-S150.PubMedGoogle Scholar
  169. Nabeshima, K., Inoue, T., Shimao, Y., Kataoka, H. and Koono, M. 1999. Cohort migration of carcinoma cells, differentiated colorectal carcinoma cells move as coherent cell clusters or sheets. Histol. Histopathol. 14, 1183-1197.PubMedGoogle Scholar
  170. Nalbant, P., Hodgson, L., Kraynov, V., Toutchkine, A. and Hahn, K. M. 2004. Activation of endogenous Cdc42 visualized in living cells. Science 305, 1615-1619.PubMedGoogle Scholar
  171. Niggli, V. 2005. Regulation of protein activities by phosphoinositide phosphates. Annu. Rev. Cell Dev. Biol. 21, 57-79.PubMedGoogle Scholar
  172. Nikolopoulos, S. N., Spengler, B. A., Kisselbach, K., Evans, A. E., Biedler, J. L. and Ross, R. A. 2000. The human non-muscle alpha-actinin protein encoded by the ACTN4 gene suppresses tumorigenicity of human neuroblastoma cells. Oncogene 19, 380-386.PubMedGoogle Scholar
  173. Nishiya, N., Kiosses, W., Han, J. and Ginsberg, M. 2005. An alpha4 integrin-pax- illin-Arf-GAP complex restricts Rac activation to the leading edge of migrating cells. Nat. Cell Biol. 7, 343-352.PubMedGoogle Scholar
  174. Nixdorf, S., Grimm, M. O., Loberg, R., Marreiros, A., Russell, P. J., Pienta, K. J. and Jackson, P. 2004. Expression and regulation of MIM (Missing In Metastasis), a novel putative metastasis suppressor gene, and MIM-B, in bladder cancer cell lines. Cancer Lett. 215, 209-220.PubMedGoogle Scholar
  175. Noske, A., Denkert, C., Schober, H., Sers, C., Zhumabayeva, B., Weichert, W., Dietel, M. and Wiechen, K. 2005. Loss of gelsolin expression in human ovarian carcinomas. Eur. J. Cancer 41, 461-469.PubMedGoogle Scholar
  176. Novy, R. E., Lin, J. L., Lin, C. S. and Lin, J. J. 1993. Human fibroblast tropomyosin isoforms: Characterization of cDNA clones and analysis of tropomyosin isoform expression in human tissues and in normal and transformed cells. Cell Motil. Cytoskeleton 25, 267-281.PubMedGoogle Scholar
  177. Nowak, D., Skwarek-Maruszewska, A., Zemanek-Zboch, M. and MalickaBlaszkiewicz, M. 2005. Beta-actin in human colon adenocarcinoma cell lines with different metastatic potential. Acta Biochim. Pol. 52, 461-468.PubMedGoogle Scholar
  178. Ohashi, K., Nagata, K., Maekawa, M., Ishizaki, T., Narumiya, S. and Mizuno, K. 2000. Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop. J. Biol. Chem. 275, 3577-3582.PubMedGoogle Scholar
  179. Ohtani, K., Sakamoto, H., Rutherford, T., Chen, Z., Satoh, K. and Naftolin, F. 1999. Ezrin, a membrane-cytoskeletal linking protein, is involved in the process of invasion of endometrial cancer cells. Cancer Lett. 147, 31-38.PubMedGoogle Scholar
  180. Oien, K. A., Vass, J. K., Downie, I., Fullarton, G. and Keith, W. N. 2003. Profiling, comparison and validation of gene expression in gastric carcinoma and normal stomach. Oncogene 22, 4287-4300.PubMedGoogle Scholar
  181. Oleynikov, Y. and Singer, R. H. 2003. Real-time visualization of ZBP1 association with beta-actin mRNA during transcription and localization. Curr. Biol. 13, 199-207.PubMedGoogle Scholar
  182. Otsubo, T., Iwaya, K., Mukai, Y., Mizokami, Y., Serizawa, H., Matsuoka, T. and Mukai, K. 2004. Involvement of Arp2/3 complex in the process of colorectal carcinogenesis. Mod. Pathol. 17, 461-467.PubMedGoogle Scholar
  183. Pantel, K. and Woelfle, U. 2004. Micrometastasis in breast cancer and other solid tumors. J. Biol. Regul. Homeost. Agents 18, 120-125.PubMedGoogle Scholar
  184. Park, P. C., Taylor, M. D., Mainprize, T. G., Becker, L. E., Ho, M., Dura, W. T., Squire, J. and Rutka, J. T. 2003. Transcriptional profiling of medulloblastoma in children. J. Neurosurg. 99, 534-541.PubMedGoogle Scholar
  185. Patel, A. S., Schechter, G. L., Wasilenko, W. J. and Somers, K. D. 1998. Overexpression of EMS1/cortactin in NIH3T3 fibroblasts causes increased cell motility and invasion in vitro. Oncogene 16, 3227-3232.PubMedGoogle Scholar
  186. Pawlak, G., McGarvey, T. W., Nguyen, T. B., Tomaszewski, J. E., Puthiyaveettil, R., Malkowicz, S. B. and Helfman, D. M. 2004. Alterations in tropomyosin isoform expression in human transitional cell carcinoma of the urinary bladder. Int. J. Cancer 110, 368-373.PubMedGoogle Scholar
  187. Pederson, T. and Aebi, U. 2005. Nuclear actin extends, with no contraction in sight. Mol. Biol. Cell 16, 5055-5060.PubMedGoogle Scholar
  188. Polet, D., Vandekerckhove, J., Ampe, C. and Lambrechts, A. 2006. Putting the biochemistry of profilins in a cellular context. Curr. Top. Biochem. Res. 8, 11-28.Google Scholar
  189. Pollard, T. D. and Borisy, G. G. 2003. Cellular motility driven by assembly and disassembly of actin filaments. Cell 112, 453-465.PubMedGoogle Scholar
  190. Prasad, G. L., Fuldner, R. A. and Cooper, H. L. 1993. Expression of transduced tropomyosin 1 cDNA suppresses neoplastic growth of cells transformed by the ras oncogene. Proc. Natl Acad. Sci. USA 90, 7039-7043.PubMedGoogle Scholar
  191. Prasad, G. L., Masuelli, L., Raj, M. H. and Harindranath, N. 1999. Suppression of src-induced transformed phenotype by expression of tropomyosin-1. Oncogene 18, 2027-2031.PubMedGoogle Scholar
  192. Pugh, C. W. and Ratcliffe, P. J. 2003. The von Hippel-Lindau tumor suppressor, hypoxia-inducible factor-1 (HIF-1) degradation and cancer pathogenesis. Semin. Cancer Biol. 13, 83-89.PubMedGoogle Scholar
  193. Pulford, K., Lamant, L., Espinos, E., Jiang, Q., Xue, L., Turturro, F., Delsol, G. and Morris, S. W. 2004. The emerging normal and disease-related roles of anaplastic lymphoma kinase. Cell. Mol. Life Sci. 61, 2939-2953.PubMedGoogle Scholar
  194. Qi, Y., Chiu, J. F., Wang, L., Kwong, D. L. and He, Q. Y. 2005. Comparative proteomic analysis of esophageal squamous cell carcinoma. Proteomics 5, 2960-2971.PubMedGoogle Scholar
  195. Quinlan, M. E., Heuser, J. E., Kerkhoff, E. and Mullins, R. D. 2005. Drosophila Spire is an actin nucleation factor. Nature 433, 382-388.PubMedGoogle Scholar
  196. Raftopoulou, M. and Hall, A. 2004. Cell migration: Rho GTPases lead the way. Dev. Biol. 265, 23-32.PubMedGoogle Scholar
  197. Ramaraj, P., Singh, H., Niu, N., Chu, S., Holtz, M., Yee, J. K. and Bhatia, R. 2004. Effect of mutational inactivation of tyrosine kinase activity on BCR/ABL-induced abnormalities in cell growth and adhesion in human hematopoietic progenitors. Cancer Res. 64, 5322-5331.PubMedGoogle Scholar
  198. Rao, J. and Li, N. 2004. Microfilament actin remodeling as a potential target for cancer drug development. Curr. Cancer Drug Targets 4, 345-354.PubMedGoogle Scholar
  199. dos Remedios, C. G., Chhabra, D., Kekic, M., Dedova, I. V., Tsubakihara, M., Berry, D. A. and Nosworthy, N. J. 2003. Actin binding proteins: Regulation of cytoskeletal microfilaments. Physiol. Rev. 83, 433-473.PubMedGoogle Scholar
  200. Rho, S. B., Chun, T., Lee, S. H., Park, K. and Lee, J. H. 2004. The interaction between E-tropomodulin and thymosin beta-10 rescues tumor cells from thymosin beta-10 mediated apoptosis by restoring actin architecture. FEBS Lett. 557, 57-63.PubMedGoogle Scholar
  201. Rhodes, D. R., Barrette, T. R., Rubin, M. A., Ghosh, D. and Chinnaiyan, A. M. 2002. Meta-analysis of microarrays: Interstudy validation of gene expression profiles reveals pathway dysregulation in prostate cancer. Cancer Res. 62, 4427-4433.PubMedGoogle Scholar
  202. Rhodes, D. R. and Chinnaiyan, A. M. 2005. Integrative analysis of the cancer transcriptome. Nat. Genet. 37(Suppl.), 31-37.Google Scholar
  203. Rhodes, D. R., Yu, J., Shanker, K., Deshpande, N., Varambally, R., Ghosh, D., Barrette, T., Pandey, A. and Chinnaiyan, A. M. 2004. Large-scale meta-analysis of cancer microarray data identifies common transcriptional profiles of neoplastic transformation and progression. Proc. Natl Acad. Sci. USA 101, 9309-9314.PubMedGoogle Scholar
  204. Robinson, B. W., Musk, A. W. and Lake, R. A. 2005. Malignant mesothelioma. Lancet 366, 397-408.PubMedGoogle Scholar
  205. Rodrigo, J. P., Garcia, L. A., Ramos, S., Lazo, P. S. and Suarez, C. 2000. EMS1 gene amplification correlates with poor prognosis in squamous cell carcinomas of the head and neck. Clin. Cancer Res. 6, 3177-3182.PubMedGoogle Scholar
  206. Roma, A. A. and Prayson, R. A. 2005. Fascin expression in 90 patients with glioblastoma multiforme. Ann. Diagn. Pathol. 9, 307-311.PubMedGoogle Scholar
  207. Rommelaere, H., Waterschoot, D., Neirynck, K., Vandekerckhove, J. and Ampe, C. 2004. A method for rapidly screening functionality of actin mutants and tagged actins. Biol. Proced. Online 6, 235-249.PubMedGoogle Scholar
  208. Ross, A. F., Oleynikov, Y., Kislauskis, E. H., Taneja, K. L. and Singer, R. H. 1997. Characterization of a beta-actin mRNA zipcode-binding protein. Mol. Cell. Biol. 17,2158-2165.PubMedGoogle Scholar
  209. Sadano, H., Inoue, M. and Taniguchi, S. 1992. Differential expression of vinculin between weakly and highly metastatic B16-melanoma cell lines. Jpn. J. Cancer Res. 83,625-630.PubMedGoogle Scholar
  210. Sadano, H., Taniguchi, S. and Baba, T. 1990. Newly identified type of beta actin reduces invasiveness of mouse B16-melanoma. FEBS Lett. 271, 23-27.PubMedGoogle Scholar
  211. Sadano, H., Taniguchi, S., Kakunaga, T. and Baba, T. 1988. cDNA cloning and sequence of a new type of actin in mouse B16 melanoma. J. Biol. Chem. 263, 15868-15871.PubMedGoogle Scholar
  212. Sahai, E. and Marshall, C. J. 2003. Differing modes of tumour cell invasion have distinct requirements for Rho/ROCK signalling and extracellular proteolysis. Nat. Cell Biol. 5, 711-719.PubMedGoogle Scholar
  213. Salgia, R., Li, J. L., Ewaniuk, D. S., Pear, W., Pisick, E., Burky, S. A., Ernst, T., Sattler, M., Chen, L. B. and Griffin, J. D. 1997. BCR/ABL induces multiple abnormalities of cytoskeletal function. J. Clin. Invest. 100, 46-57.PubMedGoogle Scholar
  214. Sanchez-Carbayo, M., Schwarz, K., Charytonowicz, E., Cordon-Cardo, C. and Mundel, P. 2003. Tumor suppressor role for myopodin in bladder cancer: Loss of nuclear expression of myopodin is cell-cycle dependent and predicts clinical out- come. Oncogene 22, 5298-5305.PubMedGoogle Scholar
  215. Santelli, G., Califano, D., Chiappetta, G., Vento, M. T., Bartoli, P. C., Zullo, F., Trapasso, F., Viglietto, G. and Fusco, A. 1999. Thymosin beta-10 gene overexpression is a general event in human carcinogenesis. Am. J. Pathol. 155, 799-804.PubMedGoogle Scholar
  216. Sattler, M., Pisick, E., Morrison, P. T. and Salgia, R. 2000. Role of the cytoskeletal protein paxillin in oncogenesis. Crit. Rev. Oncog. 11, 63-76.PubMedGoogle Scholar
  217. Sazawa, A., Watanabe, T., Tanaka, M., Haga, K., Fujita, H., Harabayashi, T., Shinohara, N., Koyanagi, T. and Kuzumaki, N. 2002. Adenovirus mediated gelsolin gene therapy for orthotopic human bladder cancer in nude mice. J. Urol. 168, 1182-1187.PubMedGoogle Scholar
  218. Schirenbeck, A., Arasada, R., Bretschneider, T., Schleicher, M. and Faix, J. 2005. Formins and VASPs may co-operate in the formation of filopodia. Biochem. Soc. Trans. 33, 1256-1259.PubMedGoogle Scholar
  219. Schumacher, N., Borawski, J. M., Leberfinger, C. B., Gessler, M. and Kerkhoff, E. 2004. Overlapping expression pattern of the actin organizers Spir-1 and formin-2 in the developing mouse nervous system and the adult brain. Gene Expr. Patterns 4,249-255.Google Scholar
  220. Scoles, D. R., Nguyen, V. D., Qin, Y., Sun, C. X., Morrison, H., Gutmann, D. H. and Pulst, S. M. 2002. Neurofibromatosis 2 (NF2) tumor suppressor schwannomin and its interacting protein HRS regulate STAT signaling. Hum. Mol. Genet. 11, 3179-3189.PubMedGoogle Scholar
  221. Scott, J. A., Shewan, A. M., den Elzen, N. R., Louriero, J. J., Gertler, F. B. and Yap, A. S. 2005. Ena/VASP proteins can regulate distinct modes of actin organization at cadherin-adhesive contacts. Mol. Biol. Cell 17, 1085-1095.PubMedGoogle Scholar
  222. Segal, E., Friedman, N., Kaminski, N., Regev, A. and Koller, D. 2005. From signatures to models: Understanding cancer using microarrays. Nat. Genet. 37, S38-S45.PubMedGoogle Scholar
  223. Shen, R., Ghosh, D. and Chinnaiyan, A. 2004. Prognostic meta-signature of breast cancer developed by two-stage mixture modeling of microarray data. BMC Genomics 5, 94.PubMedGoogle Scholar
  224. Shen, Z., Xu, L., Chen, M., Li, E., Li, J., Wu, X. and Zeng, Y. 2003. Upregulated expression of Ezrin and invasive phenotype in malignantly transformed esophageal epithelial cells. World J. Gastroenterol. 9, 1182-1186.PubMedGoogle Scholar
  225. Shestakova, E. A., Wyckoff, J., Jones, J., Singer, R. H. and Condeelis, J. 1999. Correlation of beta-actin messenger RNA localization with metastatic potential in rat adenocarcinoma cell lines. Cancer Res. 59, 1202-1205.PubMedGoogle Scholar
  226. Shieh, D., Godleski, J., Herndon, J., Azuma, T., Mercer, H., Sugarbaker, D. and Kwiatkowski, D. 1999. Cell motility as a prognostic factor in Stage I nonsmall cell lung carcinoma: The role of gelsolin expression. Cancer 85, 47-57.PubMedGoogle Scholar
  227. Singh, D., Febbo, P. G., Ross, K., Jackson, D. G., Manola, J., Ladd, C., Tamayo, P., Renshaw, A. A., D’Amico, A. V., Richie, J. P., Lander, E. S., Loda, M., Kantoff, P. W., Golub, T. R. and Sellers, W. R. 2002. Gene expression correlates of clinical prostate cancer behavior. Cancer Cell 1, 203-209.PubMedGoogle Scholar
  228. Skobe, M., Rockwell, P., Goldstein, N., Vosseler, S. and Fusenig, N. E. 1997. Halting angiogenesis suppresses carcinoma cell invasion. Nat. Med. 3, 1222-1227.PubMedGoogle Scholar
  229. Skoble, J., Auerbuch, V., Goley, E. D., Welch, M. D. and Portnoy, D. A. 2001. Pivotal role of VASP in Arp2/3 complex-mediated actin nucleation, actin branch-formation and Listeria monocytogenes motility. J. Cell Biol. 155, 89-100.PubMedGoogle Scholar
  230. Small, J. V. and Resch, G. P. 2005. The comings and goings of actin: Coupling protrusion and retraction in cell motility. Curr. Opin. Cell Biol. 17, 517-523.PubMedGoogle Scholar
  231. Small, J., Stradal, T., Vignal, E. and Rottner, K. 2002. The lamellipodium: Where motility begins. Trends Cell Biol. 12, 112-120.PubMedGoogle Scholar
  232. Smith-Beckerman, D. M., Fung, K. W., Williams, K. E., Auersperg, N., Godwin, A. K. and Burlingame, A. L. 2005. Proteome changes in ovarian epithelial cells derived from women with BRCA1 mutations and family histories of cancer. Mol. Cell. Proteomics 4, 156-168.PubMedGoogle Scholar
  233. Song, Y., Maul, R. S., Gerbin, C. S. and Chang, D. D. 2002. Inhibition of anchorageindependent growth of transformed NIH3T3 cells by epithelial protein lost in neoplasm (EPLIN) requires localization of EPLIN to actin cytoskeleton. Mol. Biol. Cell 13, 1408-1416.PubMedGoogle Scholar
  234. Sosne, G., Christopherson, P. L., Barrett, R. P. and Fridman, R. 2005. Thymosinbeta4 modulates corneal matrix metalloproteinase levels and polymorphonuclear cell infiltration after alkali injury. Invest. Ophthalmol. Vis. Sci. 46, 2388-2395.PubMedGoogle Scholar
  235. Sosne, G., Xu, L., Prach, L., Mrock, L. K., Kleinman, H. K., Letterio, J. J., Hazlett, L. D. and Kurpakus-Wheater, M. 2004. Thymosin beta 4 stimulates laminin-5 production independent of TGF-beta. Exp. Cell Res. 293, 175-183.PubMedGoogle Scholar
  236. Sporn, M. B. 1996. The war on cancer. Lancet 347, 1377-1381.PubMedGoogle Scholar
  237. Steelman, L. S., Bertrand, F. E. and McCubrey, J. A. 2004. The complexity of PTEN: Mutation, marker and potential target for therapeutic intervention. Expert Opin. Ther. Targets 8, 537-550.PubMedGoogle Scholar
  238. Stradal, T. E. and Scita, G. 2006. Protein complexes regulating Arp2/3-mediated actin assembly. Curr. Opin. Cell Biol. 18, 4-10.PubMedGoogle Scholar
  239. Strehl, S., Borkhardt, A., Slany, R., Fuchs, U. E., Konig, M. and Haas, O. A. 2003. The human LASP1 gene is fused to MLL in an acute myeloid leukemia with t(11;17)(q23;q21). Oncogene 22, 157-160.PubMedGoogle Scholar
  240. Suyama, E., Wadhwa, R., Kawasaki, H., Yaguchi, T., Kaul, S. C., Nakajima, M. and Taira, K. 2004. LIM kinase-2 targeting as a possible anti-metastasis therapy. J. Gene Med. 6, 357-363.PubMedGoogle Scholar
  241. Suzuki, H., Nagata, H., Shimada, Y. and Konno, A. 1998. Decrease in gamma-actin expression, disruption of actin microfilaments and alterations in cell adhesion systems associated with acquisition of metastatic capacity in human salivary gland adenocarcinoma cell clones. Int. J. Oncol. 12, 1079-1084.PubMedGoogle Scholar
  242. Svitkina, T. M. and Borisy, G. G. 1999. Arp2/3 complex and actin depolymerizing factor/cofilin in dendritic organization and treadmilling of actin filament array in lamellipodia. J. Cell Biol. 145, 1009-1026.PubMedGoogle Scholar
  243. Takano, T., Hasegawa, Y., Miyauchi, A., Matsuzuka, F., Yoshida, H., Kuma, K. and Amino, N. 2002. Quantitative analysis of thymosin beta-10 messenger RNA in thyroid carcinomas. Jpn. J. Clin. Oncol. 32, 229-232.PubMedGoogle Scholar
  244. Takeoka, M., Ehara, T., Sagara, J., Hashimoto, S. and Taniguchi, S. 2002. Calponin h1 induced a flattened morphology and suppressed the growth of human fibrosarcoma HT1080 cells. Eur. J. Cancer 38, 436-442.PubMedGoogle Scholar
  245. Tanaka, M., Mullauer, L., Ogiso, Y., Fujita, H., Moriya, S., Furuuchi, K., Harabayashi, T., Shinohara, N., Koyanagi, T. and Kuzumaki, N. 1995. Gelsolin: A candidate for suppressor of human bladder cancer. Cancer Res. 55, 3228-3232.PubMedGoogle Scholar
  246. Thiery, J. P. 2002. Epithelial-mesenchymal transitions in tumour progression. Nat. Rev. Cancer 2, 442-454.PubMedGoogle Scholar
  247. Thiery, J. P. 2003. Epithelial-mesenchymal transitions in development and pathologies. Curr. Opin. Cell Biol. 15, 740-746.PubMedGoogle Scholar
  248. Thor, A. D., Edgerton, S. M., Liu, S., Moore, D. H., II. and Kwiatkowski, D. J. 2001. Gelsolin as a negative prognostic factor and effector of motility in erbB-2-positive epidermal growth factor receptor-positive breast cancers. Clin. Cancer Res. 7, 2415-2424.PubMedGoogle Scholar
  249. Timpson, P., Lynch, D. K., Schramek, D., Walker, F. and Daly, R. J. 2005. Cortactin overexpression inhibits ligand-induced down-regulation of the epidermal growth factor receptor. Cancer Res. 65, 3273-3280.PubMedGoogle Scholar
  250. Tomasetto, C., Moog-Lutz, C., Regnier, C. H., Schreiber, V., Basset, P. and Rio, M. C. 1995. Lasp-1 (MLN 50) defines a new LIM protein subfamily characterized by the association of LIM and SH3 domains. FEBS Lett. 373, 245-249.PubMedGoogle Scholar
  251. Tong, G. X., Yee, H., Chiriboga, L., Hernandez, O. and Waisman, J. 2005. Fascin-1 expression in papillary and invasive urothelial carcinomas of the urinary bladder. Hum. Pathol. 36, 741-746.PubMedGoogle Scholar
  252. Unwin, R. D., Craven, R. A., Harnden, P., Hanrahan, S., Totty, N., Knowles, M., Eardley, I., Selby, P. and Banks, R. E. 2003. Proteomic changes in renal cancer and co-ordinate demonstration of both the glycolytic and mitochondrial aspects of the Warburg effect. Proteomics 3, 1620-1632.PubMedGoogle Scholar
  253. Van Ginkel, P. R., Gee, R. L., Walker, T. M., Hu, D. N., Heizmann, C. W. and Polans, A. S. 1998. The identification and differential expression of calcium-binding proteins associated with ocular melanoma. Biochim. Biophys. Acta 1448, 290-297.PubMedGoogle Scholar
  254. Van Troys, M., Dewitte, D., Goethals, M., Carlier, M. F., Vandekerckhove, J. and Ampe, C. 1996. The actin binding site of thymosin beta 4 mapped by mutational analysis. EMBO J. 15, 201-210.PubMedGoogle Scholar
  255. Van Troys, M., Vandekerckhove, J. and Ampe, C. 1999. Structural modules in actin-binding proteins: Towards a new classification. Biochim. Biophys. Acta 1448,323-348.PubMedGoogle Scholar
  256. Vandekerckhove, J., Leavitt, J., Kakunaga, T. and Weber, K. 1980. Coexpression of a mutant beta-actin and the two normal beta- and gamma-cytoplasmic actins in a stably transformed human cell line. Cell 22, 893-899.PubMedGoogle Scholar
  257. Varga, A. E., Stourman, N. V., Zheng, Q., Safina, A. F., Quan, L., Li, X., SosseyAlaoui, K. and Bakin, A. V. 2005. Silencing of the tropomyosin-1 gene by DNA methylation alters tumor suppressor function of TGF-beta. Oncogene 24, 5043-5052.PubMedGoogle Scholar
  258. van ‘t Veer, L. J., Dai, H., van de Vijver, M. J., He, Y. D., Hart, A. A., Mao, M., Peterse, H. L., van der Kooy, K., Marton, M. J., Witteveen, A. T., Schreiber, G. J., Kerkhoven, R. M., Roberts, C., Linsley, P. S., Bernards, R. and Friend, S. H. 2002. Gene expression profiling predicts clinical outcome of breast cancer. Nature 415, 530-536.PubMedGoogle Scholar
  259. Verrills, N. M., Liem, N. L., Liaw, T. Y., Hood, B. D., Lock, R. B. and Kavallaris, M. 2006. Proteomic analysis reveals a novel role for the actin cytoskeleton in vincristine resistant childhood leukemia - An in vivo study. Proteomics 6, 1681-1694.PubMedGoogle Scholar
  260. Verschueren, H., Dewit, J., De Braekeleer, J., Schirrmacher, V. and De Baetselier, P. 1994. Motility and invasive potency of murine T-lymphoma cells: Effect of microtubule inhibitors. Cell Biol. Int. 18, 11-19.PubMedGoogle Scholar
  261. Vicente-Manzanares, M., Webb, D. J. and Horwitz, A. R. 2005. Cell migration at a glance. J. Cell Sci. 118, 4917-4919.PubMedGoogle Scholar
  262. Vigneswaran, N., Wu, J., Sacks, P., Gilcrease, M. and Zacharias, W. 2005. Microarray gene expression profiling of cell lines from primary and metastatic tongue squamous cell carcinoma: Possible insights from emerging technology. J. Oral Pathol. Med. 34, 77-86.PubMedGoogle Scholar
  263. Wang, W. S., Chen, P. M., Hsiao, H. L., Wang, H. S., Liang, W. Y. and Su, Y. 2004b. Overexpression of the thymosin beta-4 gene is associated with increased invasion of SW480 colon carcinoma cells and the distant metastasis of human colorectal car- cinoma. Oncogene 23, 6666-6671.Google Scholar
  264. Wang, W., Goswami, S., Lapidus, K., Wells, A. L., Wyckoff, J. B., Sahai, E., Singer, R. H., Segall, J. E. and Condeelis, J. S. 2004a. Identification and testing of a gene expression signature of invasive carcinoma cells within primary mammary tumors. Cancer Res. 64, 8585-8594.Google Scholar
  265. Wang, W., Goswami, S., Sahai, E., Wyckoff, J. B., Segall, J. E. and Condeelis, J. S. 2005a. Tumor cells caught in the act of invading: Their strategy for enhanced cell motility. Trends Cell Biol. 15, 138-145.Google Scholar
  266. Wang, Y., Klijn, J. G., Zhang, Y., Sieuwerts, A. M., Look, M. P., Yang, F., Talantov, D., Timmermans, M., Meijer-van Gelder, M. E., Yu, J., Jatkoe, T., Berns, E. M., Atkins, D. and Foekens, J. A. 2005b. Gene-expression profiles to predict distant metastasis of lymph-node-negative primary breast cancer. Lancet 365, 671-679.Google Scholar
  267. Wang, W., Wyckoff, J. B., Frohlich, V. C., Oleynikov, Y., Huttelmaier, S., Zavadil, J., Cermak, L., Bottinger, E. P., Singer, R. H., White, J. G., Segall, J. E. and Condeelis, J. S. 2002. Single cell behavior in metastatic primary mammary tumors correlated with gene expression patterns revealed by molecular profiling. Cancer Res. 62, 6278-6288.PubMedGoogle Scholar
  268. Weigelt, B., Hu, Z., He, X., Livasy, C., Carey, L. A., Ewend, M. G., Glas, A. M., Perou, C. M. and van ‘t Veer, L. J. 2005. Molecular portraits and 70-gene prognosis signature are preserved throughout the metastatic process of breast cancer. Cancer Res. 65, 9155-9158.PubMedGoogle Scholar
  269. Weinstein, B. M. 2005. Vessels and nerves: Marching to the same tune. Cell 120, 299-302.PubMedGoogle Scholar
  270. Welch, D. R. 2004. Microarrays bring new insights into understanding of breast cancer metastasis to bone. Breast Cancer Res. 6, 61-64.PubMedGoogle Scholar
  271. Welsh, J. B., Sapinoso, L. M., Su, A. I., Kern, S. G., Wang-Rodriguez, J., Moskaluk, C. A., Frierson, H. F., Jr. and Hampton, G. M. 2001. Analysis of gene expression identifies candidate markers and pharmacological targets in prostate cancer. Cancer Res. 61, 5974-5978.PubMedGoogle Scholar
  272. Wick, W., Grimmel, C., Wild-Bode, C., Platten, M., Arpin, M. and Weller, M. 2001. Ezrin-dependent promotion of glioma cell clonogenicity, motility and invasion mediated by BCL-2 and transforming growth factor-beta2. J. Neurosci. 21, 3360-3368.PubMedGoogle Scholar
  273. Wiesner, S., Legate, K. R. and Fassler, R. 2005. Integrin-actin interactions. Cell. Mol. Life Sci. 62, 1081-1099.PubMedGoogle Scholar
  274. Wilkinson, S., Paterson, H. F. and Marshall, C. J. 2005. Cdc42-MRCK and Rho-ROCK signalling cooperate in myosin phosphorylation and cell invasion. Nat. Cell Biol. 7, 255-261.PubMedGoogle Scholar
  275. Witke, W., Sharpe, A. H., Hartwig, J. H., Azuma, T., Stossel, T. P. and Kwiatkowski, D. J. 1995. Hemostatic, inflammatory and fibroblast responses are blunted in mice acking gelsolin. Cell 81, 41-51.PubMedGoogle Scholar
  276. Wittenmayer, N., Jandrig, B., Rothkegel, M., Schluter, K., Arnold, W., Haensch, W., Scherneck, S. and Jockusch, B. M. 2004. Tumor suppressor activity of profilin requires a functional actin binding site. Mol. Biol. Cell 15, 1600-1608.PubMedGoogle Scholar
  277. Wolf, K. and Friedl, P. 2005. Functional imaging of pericellular proteolysis in cancer cell invasion. Biochimie 87, 315-320.PubMedGoogle Scholar
  278. Wolf, K., Mazo, I., Leung, H., Engelke, K., von Andrian, U. H., Deryugina, E. I., Strongin, A. Y., Brocker, E. B. and Friedl, P. 2003a. Compensation mechanism in tumor cell migration: Mesenchymal-amoeboid transition after blocking of pericellular proteolysis. J. Cell Biol. 160, 267-277.Google Scholar
  279. Wolf, K., Muller, R., Borgmann, S., Brocker, E. B. and Friedl, P. 2003b. Amoeboid shape change and contact guidance: T-lymphocyte crawling through fibrillar collagen is independent of matrix remodeling by MMPs and other proteases. Blood 102, 3262-3269.Google Scholar
  280. Wyckoff, J., Wang, W., Lin, E. Y., Wang, Y., Pixley, F., Stanley, E. R., Graf, T., Pollard, J. W., Segall, J. and Condeelis, J. 2004. A paracrine loop between tumor cells and macrophages is required for tumor cell migration in mammary tumors. Cancer Res. 64, 7022-7029.PubMedGoogle Scholar
  281. Wymann, M. and Marone, R. 2005. Phosphoinositide 3-kinase in disease: Timing, location and scaffolding. Curr. Opin. Cell Biol. 17, 141-149.PubMedGoogle Scholar
  282. Xiao, G. H., Beeser, A., Chernoff, J. and Testa, J. R. 2002. p21-activated kinase links Rac/Cdc42 signaling to merlin. J. Biol. Chem. 277, 883-886.PubMedGoogle Scholar
  283. Xie, J. J., Xu, L. Y., Zhang, H. H., Cai, W. J., Mai, R. Q., Xie, Y. M., Yang, Z. M., Niu, Y. D., Shen, Z. Y. and Li, E. M. 2005. Role of fascin in the proliferation and invasiveness of esophageal carcinoma cells. Biochem. Biophys. Res. Commun. 337, 355-362.PubMedGoogle Scholar
  284. Yager, M. L., Hughes, J. A., Lovicu, F. J., Gunning, P. W., Weinberger, R. P. and O’Neill, G. M. 2003. Functional analysis of the actin-binding protein, tropomyosin 1, in neuroblastoma. Br. J. Cancer 89, 860-863.PubMedGoogle Scholar
  285. Yamada, A., Hirose, K., Hashimoto, A. and Iino, M. 2005a. Real-time imaging of myosin II regulatory light-chain phosphorylation using a new protein biosensor. Biochem. J. 385, 589-594.Google Scholar
  286. Yamada, S., Pokutta, S., Drees, F., Weis, W. I. and Nelson, W. J. 2005b. Deconstructing the cadherin-catenin-actin complex. Cell 123, 889-901.Google Scholar
  287. Yamaguchi, H., Lorenz, M., Kempiak, S., Sarmiento, C., Coniglio, S., Symons, M., Segall, J., Eddy, R., Miki, H., Takenawa, T. and Condeelis, J. 2005. Molecular mechanisms of invadopodium formation: The role of the N-WASP-Arp2/3 complex pathway and cofilin. J. Cell Biol. 168, 441-452.PubMedGoogle Scholar
  288. Yamaguchi, H., Wyckoff, J. and Condeelis, J. 2005. Cell migration in tumors. Curr. Opin. Cell Biol. 17, 559-564.PubMedGoogle Scholar
  289. Yamamoto, T., Gotoh, M., Kitajima, M. and Hirohashi, S. 1993. Thymosin beta-4 expression is correlated with metastatic capacity of colorectal carcinomas. Biochem. Biophys. Res. Commun. 193, 706-710.PubMedGoogle Scholar
  290. Yang, M., Reynoso, J., Jiang, P., Li, L., Moossa, A. R. and Hoffman, R. M. 2004b. Transgenic nude mouse with ubiquitous green fluorescent protein expression as a host for human tumors. Cancer Res. 64, 8651-8656.Google Scholar
  291. Yang, J., Tan, D., Asch, H. L., Swede, H., Bepler, G., Geradts, J. and Moysich, K. B. 2004a. Prognostic significance of gelsolin expression level and variability in nonsmall cell lung cancer. Lung Cancer 46, 29-42.Google Scholar
  292. Yap, C. T., Simpson, T. I., Pratt, T., Price, D. J. and Maciver, S. K. 2005. The motility of glioblastoma tumour cells is modulated by intracellular cofilin expression in a concentration-dependent manner. Cell Motil. Cytoskeleton 60, 153-165.PubMedGoogle Scholar
  293. Yeo, M., Kim, D. K., Park, H. J., Oh, T. Y., Kim, J. H., Cho, S. W., Paik, Y. K. and Hahm, K. B. 2006. Loss of transgelin in repeated bouts of ulcerative colitisinduced colon carcinogenesis. Proteomics 6, 1158-1165.PubMedGoogle Scholar
  294. Yin, H. L. and Janmey, P. A. 2003. Phosphoinositide regulation of the actin cytoskeleton. Annu. Rev. Physiol. 65, 761-789.PubMedGoogle Scholar
  295. Yoder, B. J., Tso, E., Skacel, M., Pettay, J., Tarr, S., Budd, T., Tubbs, R. R., Adams, J. C. and Hicks, D. G. 2005. The expression of fascin, an actin-bundling motility protein, correlates with hormone receptor-negative breast cancer and a more aggressive clinical course. Clin. Cancer Res. 11, 186-192.PubMedGoogle Scholar
  296. Yokota, S., Yamamoto, Y., Shimizu, K., Momoi, H., Kamikawa, T., Yamaoka, Y., Yanagi, H., Yura, T. and Kubota, H. 2001. Increased expression of cytosolic chaperonin CCT in human hepatocellular and colonic carcinoma. Cell Stress Chaperones 6, 345-350.PubMedGoogle Scholar
  297. Yokoyama, M., Nishi, Y., Yoshii, J., Okubo, K. and Matsubara, K. 1996. Identification and cloning of neuroblastoma-specific and nerve tissue-specific genes through compiled expression profiles. DNA Res. 3, 311-320.PubMedGoogle Scholar
  298. Yu, Y., Khan, J., Khanna, C., Helman, L., Meltzer, P. S. and Merlino, G. 2004a. Expression profiling identifies the cytoskeletal organizer ezrin and the developmental homeoprotein Six-1 as key metastatic regulators. Nat. Med. 10, 175-181.Google Scholar
  299. Yu, Y. P., Landsittel, D., Jing, L., Nelson, J., Ren, B., Liu, L., McDonald, C., Thomas, R., Dhir, R., Finkelstein, S., Michalopoulos, G., Becich, M. and Luo, J. H. 2004b. Gene expression alterations in prostate cancer predicting tumor aggression and preceding development of malignancy. J. Clin. Oncol. 22, 2790-2799.Google Scholar
  300. Zaidel-Bar, R., Ballestrem, C., Kam, Z. and Geiger, B. 2003. Early molecular events in the assembly of matrix adhesions at the leading edge of migrating cells. J. Cell Sci. 116, 4605-4613.PubMedGoogle Scholar
  301. Zhang, H. L., Eom, T., Oleynikov, Y., Shenoy, S. M., Liebelt, D. A., Dictenberg, J. B., Singer, R. H. and Bassell, G. J. 2001. Neurotrophin-induced transport of a betaactin mRNP complex increases beta-actin levels and stimulates growth cone motility. Neuron 31, 261-275.PubMedGoogle Scholar
  302. Zicha, D., Allen, W. E., Brickell, P. M., Kinnon, C., Dunn, G. A., Jones, G. E. and Thrasher, A. J. 1998. Chemotaxis of macrophages is abolished in the Wiskott-Aldrich syndrome. Br. J. Haematol. 101, 659-665.PubMedGoogle Scholar
  303. Zigmond, S. H. 2004. Beginning and ending an actin filament: Control at the barbed end. Curr. Top. Dev. Biol. 63, 145-188.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Marleen Van Troys
    • 1
  • Joël Vandekerckhove
    • 1
  • Christophe Ampe
    • 1
  1. 1.Department of Biochemistry, Faculty of Medicine and Health SciencesGhent University and Medical Protein Research, Flanders Interuniversity Institute for Biotechnology (VIB)GentBelgium

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