Transgenics for New Plant Products, Applications to Tropical Crops

  • Samuel S.M. Sun
Part of the Plant Genetics and Genomics: Crops and Models book series (PGG, volume 1)


Advancements in plant science and agricultural technology now allow the direct transfer of gene(s) from diverse origins into target crops for improvement, with the advantages of breaking cross-species barriers and saving time in comparison to conventional breeding and selection. Transgenic technology has been used and commercialized since 1994 to produce new crop products with herbicide tolerance, insect resistance, virus resistance, and improved post-harvest quality. These input traits are characteristic of first generation transgenic crops that continue to be widely adopted by farmers globally. Numerous transgenic crop new products, with increased emphasis on output traits such as improved and novel product quality (which are more appealing and directly beneficial to the consumers), are under development and field testing. Activities in developing crops with new and better agronomic properties and using plants as bioreactors to produce high value products are also on the rise. While tropical plant germplasm, with its rich biodiversity increasingly revealed through gene discovery through genomics and associated technologies, can offer novel genes and regulatory mechanisms for crop improvement, transgenic technology provides a complementary approach with new possibilities for improving tropical crops to assure food security and nutritional well-being of the people in the tropics.


Transgenic Plant Transgenic Crop Tropical Plant Output Trait Transgenic Technology 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Agius F, Gonzalex-Lamothe R, Caballero JL, Munoz-Blanco J, Botella MA, et al. (2003) Engineering increased vitamin C levels in plants by overexpression of a D-galacturonic acid reductase. Nat Biotech 21:177–181CrossRefGoogle Scholar
  2. Agrios GN (1997) Plant Pathology, 4th edition. Academic Press, Inc., San Diego, p. 655Google Scholar
  3. Altenbach SB, Pearson KW, Leung FW, Sun SSM (1987) Cloning and sequence analysis of a cDNA encoding a Brazil nut protein exceptionally rice in methionine. Plant Mol Biol 8:239–250CrossRefGoogle Scholar
  4. Altenbach SB, Pearson KW, Meeker G, Staraci LC, Sun SSM (1989) Enhancement of the methionine content of seed proteins by the expression of a chimeric gene encoding a methionine-rich protein in transgenic plants. Plant Mol Biol 13:513–522PubMedCrossRefGoogle Scholar
  5. Ayub R, Guis M, Ben Amor M, Gillot L, Roustan JP, et al. (1996) Expression of ACC oxidase antisense gene inhibits ripening of cantaloupe melon fruits. Nat Biotech 14:862–866CrossRefGoogle Scholar
  6. Baulcombe DC (1996) Mechanisms of pathogen-derived resistance to viruses in transgenic plants. Plant Cell 8:1833–1844PubMedCrossRefGoogle Scholar
  7. Beachy RN (1997) Mechanisms and application of pathogen-derived resistance in transgenic plants. Curr Opin Plant Biotech 8:215–220CrossRefGoogle Scholar
  8. Boothe JG, Parmenter DL, Saponja JA (1997) Molecular farming in plants: oilseeds as vehicles for the production of pharmaceutical proteins. Drug Develop Res 42:172–181CrossRefGoogle Scholar
  9. Borlaug, N (2007) Sixty-two years of fighting hunger: personal recollections. Euphytica online ( Scholar
  10. Botella-Pavia P, Rodriguez-Concepcion M (2006) Carotenoid biotechnology in plants for nutrionally improved foods. Physiol Plant 126:369–381CrossRefGoogle Scholar
  11. Broun P, Gettner S, Somerville C (1999) Genetic engineering of plant lipids. Annu Rev Nutr 19:197–216PubMedCrossRefGoogle Scholar
  12. Castle LA, Wu G and McElroy D (2006) Agricultural input traits: past, present and future. Curr Opin Biotech 17:105–112PubMedGoogle Scholar
  13. Chakauya E, Coxon KM, Whitney HM, Ashurst JL, Abell C, et al. (2006) Pantothenate biosynthesis in higher plants: advance and challenges. Physiol Plant 126:319–329CrossRefGoogle Scholar
  14. Chakraborty S, Chakraborty N, Datta A (2000) Increased nutritive value of transgenic potato by expressing a nonallergenic seed albumin gene from Amaranthus hypochondriacus. Proc Natl Acad Sci USA 97:3724–3729PubMedCrossRefGoogle Scholar
  15. Coleman CE, Clore AM, Ranch JP, Higgins R, Lopes MA, et al. (1997) Expression of a mutant alpha-zein creates the floury2 phenotype in transgenic maize. Proc Natl Acad Sci USA 94:7094–7097PubMedCrossRefGoogle Scholar
  16. Cooper B, Lapidot M, Heick JA, Dodds JA, Beachy RN (1995) A defective movement protein of TMV in transgenic plants confers resistance to multiple viruses whereas the functional analog increases susceptibility. Virology 206:307–313PubMedCrossRefGoogle Scholar
  17. Daniell H, Streatfield SJ, Wycoff K (2001) Medical molecular farming: production of antibodies, biopharmaceuticals and edible vaccines in plants. Trends Plant Sci 6:219–226PubMedCrossRefGoogle Scholar
  18. Dawson WD (1996) Gene silencing and virus resistance: A common mechanism. Trends Plants Sci 1:107–108CrossRefGoogle Scholar
  19. De Block M, Botterman J, Vandewiele M, Dockx J, Thoen C, et al. (1987) Engineering herbicide resistance in plants by expression of a detoxifying enzyme. EMBO J 6:2513–2518PubMedGoogle Scholar
  20. De Clercq A, Vandewlele M, Van Damme J, Guerche P, Van Montagu M, et al. (1990) Stable accumulation of modified 2S albumin seed storage protein with higher methionine contents in transgenic plants. Plant Physiol 94:970–979PubMedCrossRefGoogle Scholar
  21. De Maagd RA, Bosch D, Stiekema W (1999) Bacillus thuringiensis toxin-mediated insect resistance in plants. Trends Plant Sci 4:9–13PubMedCrossRefGoogle Scholar
  22. De Maagd RA, Bravo A, Berry C, Crickmore N, Schnepf HE (2003) Structure, diversity, and evolution of protein toxins from spore-forming entomorpathogenic bacteria. Annu Rev Genet 37:409–433PubMedCrossRefGoogle Scholar
  23. DellaPenna D, Last RL (2006) Progress in the dissection and manipulation of plant vitamin E biosynthesis. Physiol Plant 126:356–368CrossRefGoogle Scholar
  24. Delmer DP (2005) Agriculture in the developing world: Connecting innovations in plant research to downstream application. Proc Natl Acad Sci USA 102:15739–15746PubMedCrossRefGoogle Scholar
  25. Dickinson CD, Scott MO, Hussein EHA, Argos P, Nielsen NC (1990) Effect of structural modification on the assembly of a glycinin subunit. Plant Cell 2:403–413PubMedCrossRefGoogle Scholar
  26. Federici BA (2005) Insecticidal bacteria: an overwhelming success for invertebrate pathology. J Invertebr Pathol 89:30–38PubMedCrossRefGoogle Scholar
  27. Ferreira SA, Pitz KY, Manshardt R, Zee F, Fitch M, et al. (2002) Virus coat protein transgenic papaya provides practical control of papaya ringspot virus in Hawaii. Plant Dis 86:101–105Google Scholar
  28. Fischer R, Emans N (2000) Molecular farming of pharmaceutical proteins. Transgenic Res 9:279–299PubMedCrossRefGoogle Scholar
  29. Fischer R, Drossarf J, Commandeur U, Schillberg S, Emans N (1999) Towards molecular farming in the future: moving from diagnostic protein and antibody production in microbes to plants. Biotech Appl Biochem 30:101–108Google Scholar
  30. Fischer R, Stoger E, Schillberg S, Christou P, Twyman RM (2004) Plant-based production of biopharmaceuticals. Curr Opin Plant Biol 7:152–158PubMedCrossRefGoogle Scholar
  31. Fuchs M, Gonsalves D (1995) Resistance of transgenic hybrid squash ZW-20 expressing the coat protein genes of zhucchini yellow mosaic virus and watermelon mosaic virus 2 to mixed infections by both potyviruses. Bio/Tech 13:1466–1473CrossRefGoogle Scholar
  32. Fuchs M, Gonsalves D (1997) Genetic engineering. In: Environmentally Safe Approaches to Crop Disease Control. CRC Lewis, Boca Raton, pp. 333–363Google Scholar
  33. Galili G, Galili S, Lewinsohn E, Tadmor Y (2002) Genetic, molecular, and genomic approaches to improve the value of plant foods and feeds. Crit Rev Plant Sci 21:167–204CrossRefGoogle Scholar
  34. Galili G, Hoefgen R (2002) Metabolic engineering of amino acids and storage protein in plants. Metab Engng 4:3–11CrossRefGoogle Scholar
  35. Galun E, Breiman A (1997) Transgenic plants. Imperial College Press, London, pp. 234–248Google Scholar
  36. Ghandilyan A, Vreugdenhil D, Aarts MGM (2006) Progress in the genetic understanding of plant iron and zinc nutrition. Physiol Plant 126:407–417CrossRefGoogle Scholar
  37. Goddijn OJM, Pen J (1995) Plants as bioreactors. Trends Biotech 13:379–387CrossRefGoogle Scholar
  38. Herbers K, Sonnewald U (1999) Production of new/modified proteins in transgenic plants. Curr Opin Biotech 10:163–168PubMedCrossRefGoogle Scholar
  39. Hood EE, Jilka JM (1999) Plant-based production of xenogenic protein. Curr Opin Biotech 10:382–386PubMedCrossRefGoogle Scholar
  40. Hood EE, Witcher D, Maddock S, Meyer T, Baszynski C, et al. (1997) Commercial production of avidin from transgenic maize: characterization of transformant, production, processing, extraction and purification. Mol Breeding 3:291–306CrossRefGoogle Scholar
  41. Huang J, Rozelle S, Pray C, Wang Q (2002) Plant biotechnology in China. Science 295:674–677PubMedCrossRefGoogle Scholar
  42. Ishikawa T, Dowdle J, Smirnoff N (2006) Progress in manipulating ascorbic acid biosynthesis and accumulation in plants. Physiol Plant 126:343–355CrossRefGoogle Scholar
  43. James C (2007) Global status of commercialized biotech/GM crops: 2006. ISAAA Brief No. 35–2006Google Scholar
  44. Jaynes JM, Yang MS, Espinoza NO, Dodds JH (1986) Plant protein improvement by genetic engineering: use of synthetic genes. Trends Biotech 4:314–320CrossRefGoogle Scholar
  45. Katsube T, Kurisaka N, Ogawa M, Maruyama N, Ohtsuka R, et al. (1999) Accumulation of soybean glycinin and its assembly with the glutelins in rice. Plant Physiol 120:1063–1073PubMedCrossRefGoogle Scholar
  46. Keeler SJ, Maloney CL, Webber PY, Patterson C, Hirata LY, et al. (1997) Expression of de novo high-lysine alpha-helical coiled-coil proteins may significantly increase the accumulated levels of lysine in mature seeds of transgenic tobacco plants. Plant Mol Biol 34:15–29PubMedCrossRefGoogle Scholar
  47. Kim JH, Cetiner S, Jaynes JM (1992) Enhancing the nutritional quality of crop plants: design, construction and expression of an artificial plant storage protein gene. In: Bhatnagar D, Cleveland TE (eds) Molecular Approaches to Improving Food Quality and Safety, AVI Book, New Yorkpp. 1–36Google Scholar
  48. Klee HJ, Hayford MB, Kretzmer KA, Barry GF, Kishore GM (1991) Control of ethylene synthesis by expression of a bacterial enzyme in transgenic tomato plants. Plant Cell 3:1187–1193PubMedCrossRefGoogle Scholar
  49. Kochhar, SL (1981) Tropical Crops: A Textbook of Economic Botany. Macmillan Publishers, 3rd Edition. pp. 1–17Google Scholar
  50. Ku MSB, Agarie S, Nomura M, Fukayama H, Tsuchida H, et al. (1999) High-level expression of maize phosphoenolpyruvate carboxylase in transgenic rice plants. Nat Biotech 17:76–80CrossRefGoogle Scholar
  51. Lai JS, Messing J (2002) Increasing maize seed methionine by mRNA stability. Plant J 30:295–402CrossRefGoogle Scholar
  52. Liu QQ (2002) Genetic engineering rice for increased lysine. PhD thesis, Yangzhou University and the Chinese University of Hong Kong, Hong KongGoogle Scholar
  53. Lomonossoff GL (1995) Pathogen-derived resistance to plant viruses. Annu Rev Phytopathol 33:323–343CrossRefPubMedGoogle Scholar
  54. Lucca P, Hurrell R, Potrykus I (2001) Genetic engineering approaches to improve the bioavailabilty and the level of iron in rice grains. Theor Appl Genet 102:392–397CrossRefGoogle Scholar
  55. Lucca P, Poletti S, Sautter C (2006) Genetic engineering approaches to enrich rice with iron and vitamin A. Physiol Plant 126:291–303CrossRefGoogle Scholar
  56. Malpica CA, Cervera MT, Simoens C, van Mobtagu M (1998) Engineering resistance against viral diseases in plants. Subcell Biochem 29:287–320PubMedGoogle Scholar
  57. Marcellino LH, Neshich G, Grossi de Sa MF, Krebbers E, Gander ES (1996) Modified 2S albumins with improved tryptophan content are correctly expressed in transgenic tobacco plants. FEBS Lett 385:154–158PubMedCrossRefGoogle Scholar
  58. Maruta Y, Ueki J, Saito H, Nitta N, Imaseki H (2001) Transgenic rice with reduced glutelin content by transformation with glutelin antisense gene. Mol Breed 8:273–284CrossRefGoogle Scholar
  59. Mazur B, Krebbers E, Tingey S (1999) Gene discovery and production development for grain quality traits. Science 285:372–375PubMedCrossRefGoogle Scholar
  60. Mercenier A, Wiedermann U, Breiteneder H (2001) Edible genetically modified microorganisms and plants for improved health. Curr Opin Biotech 12:510–515PubMedCrossRefGoogle Scholar
  61. Napier JA, Haslam R, Caleron MV, Michaelson LV, Beaudoin F, et al. (2006) Progress towards the production of very long-chain polyunsaturated fatty acid in transgenic plants: plant metabolic engineering comes of age. Physiol Plant 126:398–406CrossRefGoogle Scholar
  62. O'Brien IEW, Forster RLS (1994) Disruption of virus movement confers broad-spectrum resistance against systemic infection by plant viruses with a triple gene block. Proc Natl Acad Sci USA 91:10310–10314.PubMedCrossRefGoogle Scholar
  63. Padgette SR, Re DB, Barry GF, Eichholtz DE, Delannay X, et al. (1996) New weed control opportunities: development of soybeans with a Roundup ReadyR gene. In: Duke SO (ed) Herbicide-resistant Crops: Agricultural, Economic, Environmental, Regulatory and Technological Aspects. Lewis Publishers, pp. 53–84Google Scholar
  64. Paine JA, Shipton CA, Chaggar S, Howells RM, Kennedy MJ, et al. (2005) A new version of Golden Rice with increased pro-vitamin A content. Nat Biotech 23:482–487CrossRefGoogle Scholar
  65. Pujol M, Ramirez NI, Ayala M, Gavilondo JV, Rodriguez M, et al. (2005) An integral approach towards a practical application for a plant-made monoclonal antibody in vaccine purification. Vaccine 23:1833–1837PubMedCrossRefGoogle Scholar
  66. Rebeille F, Ravanel S, Jabrin S, Douce R, Storozhenko S, et al. (2006) Folates in plants: biosynthesis, distribution, and enhancement. Physiol Plant 126:330–342CrossRefGoogle Scholar
  67. Sanford JC, Johnston SA (1985) The concept of parasite-derived resistance derived resistance genes from the parasite's own genome. J Theor Biol 113:395–405CrossRefGoogle Scholar
  68. Schuler TH, Poppy GM, Kerry BR, Denholm I (1998) Insect-resistant transgenic plants. TIBECH 16:168–175Google Scholar
  69. Sheehy R, Kramer M, Hiatt W (1988) Reduction of polygalacturonase activity in tomato fruit by antisense RNA.. Proc Natl Acad Sci USA 85:8805–8809PubMedCrossRefGoogle Scholar
  70. Shintani D, DellaPenna D (1998) Elevating the vitamin E content of plants through metabolic engineering. Science 282:2098–2010PubMedCrossRefGoogle Scholar
  71. Singh J, Sharp PJ, Skerritt JH (2000) A new candidate protein for high lysine content in wheat grain. J Sci Food Agric 81:216–226CrossRefGoogle Scholar
  72. Slattery CJ, Kavakli IH, Okita TW (2000) Engineering starch for increased quantity and quality. Trends Plant Sci 5:291–298PubMedCrossRefGoogle Scholar
  73. Sparrow PAC, Irwin JA, Dale PJ, Twyman RM, Ma JKC (2007) Pharma-planta: Road testing the developing regulatory guidelines for plant-made pharmaceuticals. Transgenic Res 16:147–161PubMedCrossRefGoogle Scholar
  74. Stalker DM, McBride KE, Malyj LD (1988) Herbicide resistance in transgenic plants expressing a bacterial detoxification gene. Science 242:419–423PubMedCrossRefGoogle Scholar
  75. Sun SSM (1999) Methionine enhancement in plants. In: Singh BK (ed) Plant Amino Acids: biochemistry and biotechnology, Marcel Dekker, pp. 509–522Google Scholar
  76. Sun SSM, Larkins BA (1993) Transgenic plants for improving seed storage protein. In: Kung SD, Wu R (eds) Transgenic Plants (Vol 1) Engineering and Utilization. Academic Press, New York, pp 339–371Google Scholar
  77. Sun SSM, Wang ML, Tu HM, Zuo WN, Xiong LW, et al. (2000) Transgenic approach to improve crop quality. In: Lin ZP (ed) Green Genes for the 21st Century. Sci Pub., Beijing, pp. 207–219Google Scholar
  78. Sun SSM, Liu QQ (2004) Transgenic approaches to improve the nutritional quality of plant proteins. In Vitro Cell Dev Biol –Plants 40:55–162Google Scholar
  79. Sun SSM, Xiong LW, Jing YX, Liu BL (1993) Lysine rich protein from winged bean. US patent #5,270,200Google Scholar
  80. Takaiwa F, Katsube T, Kitagawa S, Higasa T, Kito M, et al. (1995) High level accumulation of soybean glycinin in vacuole-derived protein bodies in the endosperm tissue of transgenic tobacco seed. Plant Sci 111:39–49CrossRefGoogle Scholar
  81. Theologis A, Oeller PW, Wong LM, Rpttmann WH, Gantz DM (1993) Use of a tomato mutant constructed with reverse genetics to study fruit ripening, a complex developmental process. Develop Genet 14:282–295CrossRefGoogle Scholar
  82. Toenniessen GH (2002) Crop genetic improvement for enhanced human nutrition. J Nutr 132:2943S–2946SPubMedGoogle Scholar
  83. Tu HM, Godfrey LW, Sun SSM (1998) Expression of the Brazil nut methionine-rich protein and mutants with increased methionine in transgenic potato. Plant Mol Biol 37:829–838PubMedCrossRefGoogle Scholar
  84. Van Eenennaam AL, Lincoln K, Durrett TP, Valentin HE, Shewmaker CK, et al. (2003) Engineering vitamin E content:from Arabidopsis mutant to soy oil. Plant Cell 15:3007–3019PubMedCrossRefGoogle Scholar
  85. Waterhouse PM, Wang MB, Lough T (2001) Gene silencing as an adaptive defense against viruses. Nature 411:834–842PubMedCrossRefGoogle Scholar
  86. Whalon ME and Wingerd BA (2003) BT: mode of action and use. Arch Insect Biochem Physiol 54:200–211PubMedCrossRefGoogle Scholar
  87. Willmitzer L (1999) Plant biotechnology: output traits – the second generation of plant biotechnology products is gaining momentum. Curr Opin Biotech 10:161–162CrossRefGoogle Scholar
  88. Willmitzer L, Topfer R (1992) Manipulation of oil, starch and protein composition. Curr Opin Biotech 3:176–180CrossRefGoogle Scholar
  89. Woodard SL, Mayor JM, Bailey MR, Bailey MR, Barker DK, et al. (2003) Maize (Zea mays)-derived bovine trypsin: characterization of the first large-scale, commercial protein product from transgenic plants. Biotech Appl Biochem 38:123–130CrossRefGoogle Scholar
  90. Wu KM and Guo YY (2005) The evolution of cotton pest management practices in China. Annu Rev Entomol 50:31–52PubMedCrossRefGoogle Scholar
  91. Yang MS, Espinoza NO, Nagplala PG, Doods JH, White FF, et al. (1989) Expression of a synthetic gene for improved protein quality in transformed potato plants. Plant Sci 64:99–111CrossRefGoogle Scholar
  92. Ye X, Al-Babili S, Kloti A, Zhang J, Lucca P, et al. (2000) Engineering the provitamin A (beta-carotene) biosynthesis pathway into (carotene-free) rice endosperm. Science 287:303–305PubMedCrossRefGoogle Scholar
  93. Young VR, Pellett PL (1994) Plant proteins in relation to human protein and amino acid nutrition. Am J Clin Nutr 59:1203S–1212SPubMedGoogle Scholar
  94. Zhang P, Jaynes JM, Potrykus I, Gruissem W, Puonti-Kaerlas J. (2003) Transfer and expression of an artificial storage protein (ASP1) gene in cassava (Manihot esculata Cranz). Transgenic Res 12:243–250PubMedCrossRefGoogle Scholar
  95. Zimmermann MB, Hurrell RF (2002) Improving iron, zinc and vitamin A nutrition through plant biotechnology. Curr Opin Biotech 13:142–145PubMedCrossRefGoogle Scholar
  96. Zuo WN (1993) Sulfur-rich 2S proteins in Lechydidaceae and their methionine-enriched forms of transgenic plants. PhD thesis, University of HawaiiGoogle Scholar

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© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Samuel S.M. Sun
    • 1
  1. 1.Department of BiologyThe Chinese University of Hong KongHong KongChina

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