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Plant Cytogenetics pp 323–333Cite as

Practical Laboratory Exercises for Plant Molecular Cytogenetics

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Part of the book series: Plant Genetics and Genomics: Crops and Models ((PGG,volume 4))

Abstract

Practical laboratory work parallel with a lecture topic is one way to improve understanding of the subject matter. Cytogenetics deals mainly with chromosome behavior and biology. The key concepts of cytogenetics can be attached to actual experimental observations that can help students visualize the relationship of the concepts to chromosomes in practice. Many new technological developments are available to aid understanding of chromosome behavior, biology, and manipulation for plant and animal improvement. We present here a series of exercises designed to help students understand chromosome behavior during cellular divisions, chromosomal aberrations, and chromosome preparations for molecular cytogenetics, such as fluorescence in situ hybridization (FISH), genomic in situ hybridization (GISH), and even fiber-FISH. These exercises are used in several undergraduate and graduate courses. If they are to be incorporated into a cytogenetics course, we recommend that the instructor choose those that fit the course time-table and available resources.

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Acknowledgments

We thank the graduate and teaching assistants who have helped teach the cytogenetics laboratory at Purdue University and North Dakota State University. We also thank Drs. Jerome D. Franckowiak at North Dakota State University and Jiming Jiang at the University of Wisconsin-Madison, who previously developed much of the material presented here. Special thanks are due to our dedicated and committed cytogenetics teachers, who have helped teach a new generation about the potential of this often overlooked but powerful branch of science.

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Authors and Affiliations

  1. Department of Agronomy, Purdue University, 915 West State Street, West Lafayette, IN, 47907, USA

    Scott A. Jackson

  2. Department of Plant Sciences, North Dakota State University, 470G Loftsgard Hall, Fargo, ND, 58105, USA

    Shahryar F. Kianian

  3. Division of Science and Mathematics, Mayville State University, Mayville, ND, 58257, USA

    Khwaja G. Hossain

  4. Department of Horticulture, University of Wisconsin, 1575 Linden Drive, Madison, WI, 53706, USA

    Jason G. Walling

Authors
  1. Scott A. Jackson
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  2. Shahryar F. Kianian
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  3. Khwaja G. Hossain
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  4. Jason G. Walling
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Corresponding authors

Correspondence to Scott A. Jackson or Shahryar F. Kianian .

Editor information

Editors and Affiliations

  1. Department of Biological Science, Florida State University, Tallahassee, Florida, USA

    Hank W. Bass

  2. Division of Biological Sciences, University of Missouri, Columbia, Missouri, USA

    James A. Birchler

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Jackson, S.A., Kianian, S.F., Hossain, K.G., Walling, J.G. (2012). Practical Laboratory Exercises for Plant Molecular Cytogenetics. In: Bass, H., Birchler, J. (eds) Plant Cytogenetics. Plant Genetics and Genomics: Crops and Models, vol 4. Springer, New York, NY. https://doi.org/10.1007/978-0-387-70869-0_15

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