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Mutagenesis Systems for Genetic Analysis of Gossypium

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Genetics and Genomics of Cotton

Part of the book series: Plant Genetics and Genomics: Crops and Models ((PGG,volume 3))

Abstract

The recent evolution of tetraploid cotton combined with intensive selection of cultivated cottons has reduced the genetic diversity of cotton. This lack of allelic diversity hampers efforts to improve the agronomic traits of cotton and limits the application of molecular genetic tools for improvement of cotton germplasm. The lack of genetic resources also reduces our ability to understand the molecular mechanisms that regulate cotton growth and development and its responses to environmental stresses and pathogens. Use of a variety of chemical mutagens and ionizing radiation can be used to effectively increase the frequency of mutant alleles in Gossypium species. While application of insertional mutagenesis methodologies that require high-throughput plant transformation procedures is not feasible, evaluation of various transposon-based mutagenesis systems is underway. TILLING technology, which uses a combination of mutagenesis and high-throughput molecular screening methods for reverse genetics is also being developed.

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Acknowledgments

The authors thank Haggag Abdel-Mageed and Bay Nguyen for their work on transposon mutagenesis and TILLING, respectively. The authors acknowledge the generous support from Cotton Inc.

Authors
  1. Dick Auld
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  2. Ginger G. Light
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  3. Mohamed Fokar
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  4. Efrem Bechere
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  5. Randy D. Allen
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Editors and Affiliations

  1. Plant Genome Mapping Laboratory, University of Georgia, 111 Riverbend Road, 30602, Athens, GA, USA

    Andrew H. Paterson

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Auld, D., Light, G.G., Fokar, M., Bechere, E., Allen, R.D. (2009). Mutagenesis Systems for Genetic Analysis of Gossypium . In: Paterson, A.H. (eds) Genetics and Genomics of Cotton. Plant Genetics and Genomics: Crops and Models, vol 3. Springer, New York, NY. https://doi.org/10.1007/978-0-387-70810-2_9

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