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Tissue Microarrays in Cancer Research

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Modern Molecular Biology

Abstract

Tissue microarrays (TMAs) are composite tissue blocks capable of accommodating over 1,000 unique tissue cores on a single glass slide. TMAs have become widely adopted in pathology and biomarker research. This chapter briefly discusses the design and construction of TMAs, the state of TMA imaging, and current methods for the analysis and management of TMA data. A significant portion of the chapter highlights the technical challenges of using formalin-fixed, paraffin embedded tissue and analyzing tissue stained using immunohistochemistry (IHC).

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Authors and Affiliations

  1. Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Angelo M. De Marzo

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Correspondence to Angelo M. De Marzo .

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  1. Sidney Kimmel Comprehensive, Cancer Center, Johns Hopkins University, Orleans Street 1650, Baltimore, 21231, Maryland, USA

    Srinivasan Yegnasubramanian

  2. School of Medicine, Urology, Pharmacology, Oncology, Johns Hopkins University, N. Wolfe Street 600, Baltimore, 21287, Maryland, USA

    William B. Isaacs

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Cornish, T.C., De Marzo, A.M. (2010). Tissue Microarrays in Cancer Research. In: Yegnasubramanian, S., Isaacs, W. (eds) Modern Molecular Biology. Applied Bioinformatics and Biostatistics in Cancer Research. Springer, New York, NY. https://doi.org/10.1007/978-0-387-69745-1_8

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