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Murine Models of Hematopoietic Disease: Pathologic Analysis and Characterization

  • Benjamin H. Lee
  • Jeffery L. Kutok
Chapter

Introduction

The pathologic evaluation of murine hematologic disease can be considerably more challenging than the assessment of human hematopoietic malignancies. Diagnoses in humans that may be difficult based solely on histology are often straightforward with careful review of the patient’s laboratory data or after specialized ancillary testing. Unfortunately, such luxuries do not always exist in the workup of a mouse. Obtaining important clinical and laboratory data for each mouse in a cohort is often difficult and costly. Peripheral blood counts and cytologic findings in the blood and marrow play a critical role in the diagnosis of disease in both mice and humans; however, quite often, a mouse is simply fixed in formalin enabling only histopathologic review. Immunophenotyping by flow cytometry or cytochemical studies require fresh cells and are necessary to confirm morphologic impressions. This testing should be a part of all murine hematopoietic workups but needs to be planned and...

Keywords

Acute Myeloid Leukemia Chronic Myeloid Leukemia Polycythemia Vera Essential Thrombocythemia Noonan Syndrome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Benjamin H. Lee
  • Jeffery L. Kutok
    • 1
  1. 1.Brigham and Women’s Hospital, Harvard Medical SchoolDepartment of PathologyBostonUSA

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