Kinetochores are the multiprotein macromolecular assemblies on chromatin that ensure the accurate and timely segregation of chromosomes at during mitosis. To achieve this, kinetochores must interact with the microtubules of the spindle and microtubule-associated proteins. The nature of the kinetochore–microtubule interaction varies during the stages of the mitotic cycle, starting with initial capture and progressing through bi-orientation and congression at prometaphase/metaphase, then finally separation of sister kinetochores/chromatids during anaphase. All the while during this process, kinetochores are able to signal their state of microtubule binding to the cell cycle control machinery. They are also able to influence microtubule dynamics in order to achieve chromosome segregation. Determining the structure and biochemistry of these various interactions continues to be a major objective of research in this field.
Much of the cell biology/cytology of cell division has...
KeywordsSpindle Pole Spindle Assembly Checkpoint Sister Kinetochore Chromosomal Passenger Complex Microtubule Attachment
Work in the authors’ laboratory was supported by Cancer Research UK, the Wellcome Trust, the Human Frontier Science Program, the Lister Research Institute Prize and the Association for International Cancer Research. T.U. Tanaka is a Senior Research Fellow of Cancer Research UK.
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