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The Role of the Kinetochore in Spindle Checkpoint Signaling

  • P. Todd Stukenberg
  • Daniel J. Burke
Chapter

Background

It was observed, with the advent of live cell imaging in the 1950s, that cells did not enter into anaphase until the last chromosomes arrived at the metaphase plate suggesting intricate regulation between chromosome movements and cell cycle progression (Carlson, 1956; Bajer and Mole-Bajer, 1961). Spermatocytes of praying mantids provided a dramatic demonstration of this intricate regulation (Callan and Jacobs, 1957). Male mantids have X1X2Y sex determination, a result of an ancient event that split the X chromosome in two. During meiosis the X1X2Y trivalent must disjoin to produce an X1X2–containing gamete and a Y-containing gamete. However, there is chromosome misalignment in 10% of meiotic divisions producing an X-Y bivalent and one X chromosome that is unaligned at metaphase. Interestingly, cells with the unaligned X chromosome remain arrested at metaphase of meiosis I. Callan and Jacobs (1957) proposed that cells sense the single unaligned chromosome and inhibit the...

Keywords

Kinetochore Protein Spindle Checkpoint Checkpoint Protein Microtubule Binding Sister Kinetochore 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank Sue Biggins and the members of the Stukenberg and Burke labs for helpful discussions and comments on the manuscript. We also thank Mitsuhiro Yanagida, Mark Jackman, and Jonathon Pines for communicating results prior to publication. We apologize to those colleagues whose work was not cited due to space limitations.

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© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Department of Biochemistry and Molecular GeneticsUniversity of Virginia Medical CenterCharlottesvilleU.S.A

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