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Sample Shrinkage and Radiation Damage of Plastic Sections

  • Pradeep K. Luther

Abstract

Just as fossil insects embalmed in amber are extraordinarily preserved, so are biological samples that have been embedded in plastic for electron microscopy. The success of embedding samples in plastic lies in the astounding resilience of the sections in the electron microscope, albeit after initial changes. The electron microscope image results from projection of the sample density in the direction of the beam, i.e. through the depth of the section, and therefore is independent of physical changes in this direction. In contrast, the basis of electron tomography is the constancy of the physical state of the whole section during the time that different views at incremental tilt angle steps are recorded.

Keywords

Gold Particle Radiation Damage Electron Tomography Section Thickness Rapid Freezing 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Pradeep K. Luther
    • 1
  1. 1.Biomedical Sciences DivisionImperial College LondonLondonUK

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