In 1984, the B95-8 isolate of Epstein–Barr virus (EBV), a commonly used laboratory strain whose DNA genome had been partially or completely cloned by several groups, was the first herpesvirus to have its genome completely sequenced (Baer et al. 1984). The information gained from this first genomic sequence (accession number V01555) provided a wealth of new information on the coding potential of this DNA tumor virus, and consequently was the basis for the rapid advancement of the EBV field that soon followed. The complete sequence data were particularly critical, for example, to the characterization of the complex latency-associated genes of EBV, whose highly spliced mRNAs span upward of 85 kilobase pairs (kbp) of the genome. Sequence information missing from the B95-8 genome – the result of a 12-kbp deletion – was subsequently provided by analysis of the corresponding genomic region of the Raji EBV isolate (Parker et al. 1990). An updated and fully annotated wild-type EBV...
KeywordsEBNA Expression BHRF1 miRNAs
Much of the material presented in Fig. 11.2 and Table 11.1 was adopted from information available at http://www.med.ic.ac.uk/ludwig/ebv.htm that is maintained by Dr. Paul Farrell. We apologize to our many colleagues whose work could not be fully cited due to space constraints. We gratefully acknowledge our research support from U.S. Public Health Service grants CA056639 and CA073544 (to J.T.S.), CA056645 and CA117827 (to C.E.S.), and the Penn State Hershey Cancer Institute.
- Austin, P.J., Flemington, E., Yandava, C.N., Strominger, J.L. and Speck, S.H. (1988) Complex transcription of the Epstein-Barr virus BamHI fragment H rightward open reading frame 1 (BHRF1) in latently and lytically infected B lymphocytes. Proc. Natl. Acad. Sci. USA 85, 3678–3682.PubMedCrossRefGoogle Scholar
- Cai, X., Schafer, A., Lu, S., Bilello, J.P., Desrosiers, R.C., Edwards, R., Raab-Traub, N. and Cullen, B.R. (2006) Epstein-Barr virus microRNAs are evolutionarily conserved and differentially expressed. PLoS Pathog. 2, 236–247.Google Scholar
- Kelly, G.L., Milner, A.E., Tierney, R.J., Croom-Carter, D.S.G., Altmann, M., Hammerschmidt, W., Bell, A.I. and Rickinson, A.B. (2005) Epstein-Barr virus nuclear antigen 2 (EBNA2) gene deletion is consistently linked with EBNA-3A, -3B, and -3C expression in Burkitt's lymphoma cells and with increased resistance to apoptosis. J. Virol. 79, 10709–10717.Google Scholar
- Nonkwelo, C., Skinner, J., Bell, A., Rickinson, A. and Sample, J. (1996) Transcription start sites downstream of the Epstein-Barr virus (EBV) Fp promoter in early-passage Burkitt lymphoma cells define a fourth promoter for expression of the EBV EBNA-1 protein. J. Virol. 70, 623–627.PubMedGoogle Scholar
- Rivailler, P., Cho, Y.G. and Wang, F. (2005) Complete genomic sequence of an Epstein-Barr virus-related herpesvirus naturally infecting a New World primate: a defining point in the evolution of oncogenic lymphocryptoviruses. J. Virol. 76, 12055–12068.Google Scholar
- Schaefer, B.C., Strominger, J.L. and Speck, S.H. (1995) Redefining the Epstein-Barr virus-encoded nuclear antigen EBNA-1 gene promoter and transcription initiation site in group I Burkitt lymphoma cell lines. Proc. Natl. Acad. Sci. USA 92, 10565–10569.Google Scholar
- Sears, J., Ujihara, M., Wong, S., Ott, C., Middeldorp, J. and Aiyar, A. (2004) The amino terminus of Epstein-Barr Virus (EBV) nuclear antigen 1 contains AT hooks that facilitate the replication and partitioning of latent EBV genomes by tethering them to cellular chromosomes. J. Virol. 78, 11487–11505.Google Scholar
- Wu, Y., Maruo, S., Yajima, M., Kanda, T. and Takada, K. (2007) Epstein-Barr virus (EBV)-encoded RNA 2 (EBER2) but not EBER1 plays a critical role in EBV-induced B-cell growth transformation. J. Virol. 81, 11236–11245.Google Scholar
- Yao, Q.Y., Croom-Carter, D.S., Tierney, R.J., Habeshaw, G., Wilde, J.T., Hill, F.G., Conlon, C. and Rickinson, A.B. (1998) Epidemiology of infection with Epstein-Barr virus types 1 and 2: lessons from the study of a T-cell-immunocompromised hemophilic cohort. J. Virol. 72, 4352–4363.PubMedGoogle Scholar
- Zeng, M.S., Li, D.J., Liu, Q.L., Song, L.B., Li, M.Z., Zhang, R.H., Yu, X.J., Wang, H.M., Ernberg, I. and Zeng, Y.X. (2005) Genomic sequence analysis of Epstein-Barr virus strain GD1 from a nasopharyngeal carcinoma patient. J. Virol. 79, 15323–15330.Google Scholar