Applications of Dielectrophoresis-based Lab-on-a-chip Devices in Pharmaceutical Sciences and Biomedicine

  • Claudio Nastruzzi
  • Azzurra Tosi
  • Monica Borgatti
  • Roberto Guerrieri
  • Gianni Medoro
  • Roberto Gambari
Part of the Series on Integrated Circuits and Systems book series (ICIR)


Whisper Gallery Mode Rare Cell Melamine Resin Glycerol Monostearate Cetyl Palmitate 
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  1. [1]
    G. Gastrock, K. Lemke, J. Metze, “Sampling and monitoring in bioprocess- ing using microtechniques,” J Biotechnol, vol. 82, pp. 123-135, 2001.Google Scholar
  2. [2]
    H.M. Haake, A. Schutz, G. Gauglitz, “Label-free detection of biomolecular interaction by optical sensors,” Fresenius J Anal Chem, vol. 366, pp. 576-585, 2000.CrossRefGoogle Scholar
  3. [3]
    R.C. McGlennen, “Miniaturization technologies for molecular diagnostics,” Clin Chem, vol. 47, pp. 393-402, 2001.Google Scholar
  4. [4]
    R.L. Rich, D.G. Myszka, “Survey of the year 2000 commercial optical bio- sensor literature”, J Mol Recogn, vol. 14, pp. 273-294, 2001.CrossRefGoogle Scholar
  5. [5]
    P. Fortina, S. Surrey, L.J. Kricka, “Molecular diagnostics: hurdles for clinical implementation,”, Trends Mol Med, vol. 8, pp. 264-266, 2002.CrossRefGoogle Scholar
  6. [6]
    K.K. Jain, “Cambridge Healthtech Institute’s Third Annual Conference on Lab-on-a-Chip and Microarrays,” Zurich, Switzerland, Pharmacogenomics, vol. 2, pp. 73-77, 2001.CrossRefGoogle Scholar
  7. [7]
    L.J. Kricka, “Microchips, microarrays, biochips and nanochips: Personal laboratories for the 21st century,” Clin Chim Acta, vol. 307, pp. 219-223, 2001.CrossRefGoogle Scholar
  8. [8]
    N. Manaresi, A. Romani, G. Medoro, L. Altomare, A. Leonardi, M. Tartagni, R. Guerrieri, “A CMOS Chip for Individual Cell Manipulation and Detection,” 2003 IEEE ISSCC Dig Tech Papers, pp. 192-193, 2003.Google Scholar
  9. [9]
    H. Morgan, M.P. Hughes, N.G. Green, “Separation of submicron bioparticles by dielectrophoresis,” Biophys J, vol. 77, pp. 516-525, 1999.CrossRefGoogle Scholar
  10. [10]
    J. Voldman, R.A. Braff, M. Toner, M.L. Gray, M.A. Schmidt, “Holding for- ces of single-particle dielectrophoretic traps,” Biophys J, vol. 80, pp. 531-541, 2001.CrossRefGoogle Scholar
  11. [11]
    L.J. Kricka, “Microchips, microarrays, biochips and nanochips: personal laboratories for the 21st century”, Clin Chim Acta, vol. 307, pp. 219-223, 2001.CrossRefGoogle Scholar
  12. [12]
    P. Hawtin, I. Hardern, R. Wittig, J. Mollenhauer, A. Poustka, R. Salowsky, T. Wulff, C. Rizzo, B. Wilson, “Utility of lab-on-a-chip technology for highthroughput nucleic acid and protein analysis,” Electrophoresis, vol. 26, pp. 3674-3681, 2005CrossRefGoogle Scholar
  13. [13]
    H. Goetz, M. Kuschel, T. Wulff, C. Sauber, C. Miller, S. Fisher, C. Woodward, “Comparison of selected analytical techniques for protein sizing, quantitation and molecular weight determination,” J Biochem Biophys Methods, vol. 60, pp. 281-293, 2004.CrossRefGoogle Scholar
  14. [14]
    L. Shi, W. Tong, F. Goodsaid, F.W. Frueh, H. Fang, T. Han, J.C. Fuscoe, D.A: Casciano, “QA/QC: challenges and pitfalls facing the microarray community and regulatory agencies,” Expert Rev Mol Diagn, vol. 4, pp. 761-777, 2004.CrossRefGoogle Scholar
  15. [15]
    T. Muller, A. Pfennig, P. Klein, G. Gradl, M. Jager, T. Schnelle, “The poten-tial of dielectrophoresis for single-cell experiments,” IEEE Eng Med Biol, vol. 22, pp. 51-61, 2003.CrossRefGoogle Scholar
  16. [16]
    J. Yang, Y. Huang, X.B. Wang, F.F. Becker, P.R. Gascoyne, “Cell separation on microfabricated electrodes using dielectrophoretic/gravitational field-flow fractionation,” Anal Chem, vol. 71, pp. 911-918, 1999.CrossRefGoogle Scholar
  17. [17]
    P.R. Gascoyne, J. Vykoukal, “Particle separation by dielectrophoresis,” Electrophoresis, vol. 23, pp. 1973-1983, 2002.CrossRefGoogle Scholar
  18. [18]
    T. Schnelle, T. Müller, G. Gradl, G. Shirley, G. Fuhr, “Paired Microelectrode System: Dielectrophoretic Particle Sorting and Force Calibration,” J Electrost, vol. 47, pp. 121-132, 1999.CrossRefGoogle Scholar
  19. [19]
    N. Manaresi, A. Romani, G. Medoro, L. Altomare, A. Leonardi, M. Tartagni, R. Guerrieri, “A CMOS Chip for Individual Cell Manipulation and Detection,” IEEE J Solid-State Circuit, vol. 38, pp. 2297-2305, 2003.CrossRefGoogle Scholar
  20. [20]
    H.A. Pohl, “The Motion and the Precipitation of Suspensoid in Divergent Electric Fields,” J Appl Phys D: Appl Phys, vol. 22, pp. 869-871, 1951.Google Scholar
  21. [21]
    G. Fuhr, H. Glasser, T. Müller, T. Schnelle, “Cell Manipulation and Cultivation Under A.C. Electric Field Influence in Highly Conductive Culture Media,” Biochim. and Biophys. Acta, vol. 1201, pp. 353-360, 1994.Google Scholar
  22. [22]
    R. Pethig, Y. Huang, X. Wang, J.P.H. Burt,“Positive and Negative Dielectrophoretic Collection of Colloidal Particles Using Interdigitated Castellated Microelectrodes,” J Phys D: Appl Phys, vol. 25, pp. 881-888, 1992.CrossRefGoogle Scholar
  23. [23]
    H.A. Pohl, Dielectrophoresis, Cambridge University Press, 1978.Google Scholar
  24. [24]
    H. Morgan, N.G. Green, AC Electrokinetics: colloids and nanoparticles, Research Studies Press, 2003.Google Scholar
  25. [25]
    G. Medoro, N. Manaresi, M. Tartagni, R. Guerrieri, “ CMOS-only sensor and manipulator for microorganisms,” Proceedings IEDM, pp. 415-418, 2000.Google Scholar
  26. [26]
    G. Medoro, N. Manaresi, A. Leonardi, L. Altomare, M. Tartagni, R. Guerrieri, “A Lab-on-a-chip for cell detection and manipulation,” Proceedings IEEE Sensors Conference, 2002.Google Scholar
  27. [27]
    G. Medoro, N. Manaresi, M. Tartagni, L. Altomare, A. Leonardi, R. Guerrieri, “A Lab-on-a-chip for cell separation based on the moving-cages approach,” Eurosensors, 2002.Google Scholar
  28. [28]
    X.B. Wang, Y. Huang, X. Wang, F.F. Becker, P.R. Gascoyne , “Dielectrophoretic manipulation of cells with spiral electrodes,” Biophys J, vol. 72, pp. 1887-1899, 1997.CrossRefGoogle Scholar
  29. [29]
    L. Altomare, M. Borgatti, G. Medoro, N. Manaresi, M. Tartagni, R. Guerrieri, R. Gambari, “Levitation and Movement of Human Tumor Cells using a Printed Circuit Board Device Based on Software-controlled Dielectrophoresis,” Biotechnol Bioeng, vol. 82, pp. 474-479, 2003.CrossRefGoogle Scholar
  30. [30]
    Y. Huang, S. Joo, M. Duhon, M. Heller, B. Wallace, X. Xu, “Dielectrophoretic cell separation and gene expression profiling on microelectronic chip arrays,” Anal Chem, vol. 74, pp. 3362-3371, 2002.CrossRefGoogle Scholar
  31. [31]
    J.O. Koskinen, N.J. Meltola, E. Soini, A.E. Soini, “A lab-on-a-chip compat- ible bioaffinity assay method for human alpha-fetoprotein,” Lab Chip, vol. 5, pp. 1408-1411, 2005.CrossRefGoogle Scholar
  32. [32]
    M. Borgatti, L. Altomare, M. Abonnec, E. Fabbri, N. Manaresi, G. Medoro, A. Romani, M. Tartagni, C. Nastruzzi, S. Di Croce, A. Tosi, I. Mancini, R. Guerrieri, R. Gambari, “Dielectrophoresis-based ‘Lab-on-a-chip’ devices for programmable binding of microspheres to target cells,” Int J Onc, vol. 27, pp. 1559-1566, 2005.Google Scholar
  33. [33]
    N. Chronis, L.P. Lee, “Total internal reflection-based biochip utilizing a polymer-filled cavity with a micromirror sidewall,” Lab Chip, vol. 4, pp. 125-130, 2004.CrossRefGoogle Scholar
  34. [34]
    F. Vollmer, S. Arnold, D. Braun, I. Teraoka, A. Libchaber, “Multiplexed DNA quantification by spectroscopic shift of two microsphere cavities,” Biophys J, vol. 85, pp. 1974-1979, 2003.CrossRefGoogle Scholar
  35. [35]
    R. Cortesi, E. Esposito, G. Luca, C. Nastruzzi, “Production of lipospheres as carriers for bioactive compounds,” Biomaterials, vol. 23, pp. 2283-2294, 2002.CrossRefGoogle Scholar
  36. [36]
    Claudio Nastruzzi (Editor), Lipospheres in drug targets and delivery, CRC Press, Boca Raton, 2005.Google Scholar
  37. [37]
    S. Di Croce, A. Tosi, L. Bilancetti, S. Mazzitelli, N. Bozzuto, M. Borgatti, E. Fabbri, I. Mancini, R. Gambari, C. Nastruzzi, “Cellulose acetate mic roparticles for Lab-on-a-chip applications,” New trends in bioencapsulationMinerva Biotecnologica, vol. 17, pp. 259-269, 2005.Google Scholar
  38. [38]
    S. Fiedler, S.G. Shirley, T. Schnelle, G. Fuhr, “Dielectrophoretic sorting of particles and cells in a microsystem,” Anal Chem, vol. 70, pp. 1909-1915, 1998.CrossRefGoogle Scholar
  39. [39]
    R. Gambari, M. Borgatti, L. Altomare, N. Manaresi, G. Medoro, A. Romani, M. Tartagni, R. Guerrieri, “Applications to cancer research of lab-on-a-chip devices based on dielectrophoresis (DEP),” Technol Cancer Res Treat, vol. 2, pp. 31-40, 2003.Google Scholar
  40. [40]
    Z. Yu, G. Xiang, L. Pan, L. Huang, Z. Yu, W. Xing, J. Cheng, “Negative dielectrophoretic force assisted construction of ordered neuronal networks on cell positioning bioelectronic chips,” Biomed Microdevices, vol. 6, pp. 311-324, 2004.CrossRefGoogle Scholar
  41. [41]
    C. Xu, Y. Wang, M. Cao, Z. Lu, “Dielectrophoresis of human red cells in microchips,” Electrophoresis, vol. 20, pp. 1829-1831, 1999.CrossRefGoogle Scholar
  42. [42]
    Y. Huang, J. Yang, X.B. Wang, F.F. Becker, P.R. Gascoyne, “The removal of human breast cancer cells from hematopoietic CD34+ stem cells by dielectrophoretic field-flow-fractionation,” J Hematother Stem Cell Res, vol . 8, pp. 481-490, 1999.CrossRefGoogle Scholar
  43. [43]
    G.H. Markx, M.S. Talary, R. Pethig, “Separation of viable and non-viable yeast using dielectrophoresis,” J Biotechnol, vol. 32, pp. 29-37, 1994.CrossRefGoogle Scholar
  44. [44]
    P. Gascoyne, C. Mahidol, M. Ruchirawat, J. Satayavivad, P. Watcharasit, F.F. Becker, “Microsample preparation by dielectrophoresis: isolation of malaria,” Lab Chip, vol. 2, pp. 70-75, 2002.CrossRefGoogle Scholar
  45. [45]
    P. Gascoyne, J. Satayavivad, M. Ruchirawat, “Microfluidic approaches to malaria detection,” Acta Trop, vol. 89, pp. 357-369, 2004.CrossRefGoogle Scholar
  46. [46]
    M. Borgatti, L. Altomare, M. Baruffa, E. Fabbri, G. Breveglieri, G. Feriotto, N. Manaresi, G. Medoro, A. Romani, M. Tartagni, R. Gambari, R. Guerrieri, “Separation of white blood cells from erythrocytes on a dielectrophoresis (DEP) based ‘Lab-on-a-chip’ device,” Int J Mol Med, vol. 15, pp. 913-920, 2005.Google Scholar
  47. [47]
    X. Hu, P.H. Bessette, J. Qian, C.D. Meinhart, P.S. Daugherty, H.T. Soh, “Marker-specific sorting of rare cells using dielectrophoresis,” Proc Natl Acad Sci USA, vol. 102, pp. 15757-15761, 2005.CrossRefGoogle Scholar
  48. [48]
    A.B. Fuchs, A. Romani, D. Freida, G. Medoro, M. Abonnenc, L. Altomare, I. Chartier, D. Guergour, C. Villiers, P.N. Marche, M. Tartagni, R. Guerrieri, . . Chatelain, N. Manaresi, “Electronic sorting and recovery of single live cells from microlitre sized samples,” Lab Chip, vol. 6, pp. 121-126, 2006.CrossRefGoogle Scholar
  49. [49]
    R. Thewes, F. Hofmann, A. Frey, B. Holzapfl, M. Schienle, C. Paulus et al., “Sensor Arrays for Fully-Electronic DNA Detection on CMOS,” 2002 IEEE ISSCC Dig Tech Papers, pp. 350-351, 2002Google Scholar
  50. [50]
    M. Xue, J. Li, Z. Lu, C. Feng, Z. Zhang, P.K. Ko et al., “A High-Density Conduction Based Micro DNA Identification Array Fabricated with a CMOS Compatible Process,” IEEE Trans Electron Devices, vol. 50, pp. 2165-2170, 2003.CrossRefGoogle Scholar
  51. [51]
    B. Eversmann, M. Jenkner, F. Hofmann, C. Paulus, R. Brederlow, B. Holzapfl, P. Fromherz, M. Merz, M. Brenner, M. Schreiter, R. Gabl, K. Plehnert, M. Steinhauser, G. Eckstein, D. Schmitt-Landsiedel, R. Thewes, “A 128 ×128 CMOS Biosensor Array for Extracellular Recording of Neural Activity,” IEEE J Solid-State Circuit, vol. 38, pp. 2306-2317, 2003.CrossRefGoogle Scholar
  52. [52]
    A. Romani, N. Manaresi, L. Marzocchi, G. Medoro, A. Leonardi, L. Altomare, M. Tartagni, R. Guerrieri, “Capacitive Sensor Array for Localization of Bioparticles in CMOS Lab-on-a-chip,” 2004 IEEE ISSCC Dig Tech Papers, pp. 224-225, 2004.Google Scholar
  53. [53]
    Aldo Romani, Roberto Guerrieri, Marco Tartagni, Nicolò Manaresi, Gianni Medoro, “Beyond the Microscope: Embedded Detectors for Cell Biology Applications;” Proc of ISCAS, 2005.Google Scholar
  54. [54]
    M.J. Madou, R. Cubicciotti, “Scaling Issues in Chemical and Biological Sensors,” Proc IEEE, vol. 91, pp. 830-838, 2003.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Claudio Nastruzzi
    • 1
  • Azzurra Tosi
    • 2
  • Monica Borgatti
    • 3
  • Roberto Guerrieri
    • 4
  • Gianni Medoro
    • 5
  • Roberto Gambari
    • 6
  1. 1.Department of Chemistry and Technology of DrugsUniversity of PerugiaItaly
  2. 2.Department of Chemistry and Technology of DrugsUniversity of PerugiaItaly
  3. 3.ER-GenTech, Biotechnology Center, Department of Biochemistry and MolecularUniversity of FerraraItaly
  4. 4.Center of Excellence on Electronic Systems (ARCES)University of BolognaItaly
  5. 5.Silicon BiosystemsS.p.A.Italy
  6. 6.ER-GenTech, Biotechnology Center, Department of Biochemistry and MolecularUniversity of FerraraItaly

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