Linkage Analysis and Map Construction
Linkage is the tendency for genes to be inherited together because they are located near one another on the same chromosome. Linkage analysis of markers lays a foundation for the construction of a genetic linkage map and the subsequent molecular dissection of quantitative traits using the map. Linkage analysis is based on the cosegregation of adjacent markers and their cotransmission to the next progeny generation. The prerequisite of linkage analysis between any two markers is their known allelic arrangements (i.e., linkage phases) on the homologous chromosome so that parental (or nonrecombinant) vs. nonparental (or recombinant) haplotypes can be readily distinguished. In many domesticated plants and animals, phase-known mapping pedigrees can be established using a segregating population, such as the backcross or F2, derived from two homologous inbred lines. (Recall the definitions in Section 1.5.) Theories for linkage analysis in such segregating pedigrees have been well-developed.
KeywordsLinkage Analysis Doubled Haploid Doubled Haploid Population Recombination Fraction Marker Pair
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