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References
Anliker B. and Chun J.-(2004). Cell surface receptors in lysophospholipid signaling. Semin. Cell Dev. Biol. 15:457–465.
Aoki J., Nagai Y., Hosono H., Inoue K., and Arai H. (2002). Structure and function of phosphatidylserine-specific phospholipase A1. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1582:26–32.
Balsinde J.-(2002). Roles of various phospholipases A2 in providing lysophospholipid acceptors for fatty acid phospholipid incorporation and remodelling. Biochem. J. 364:695–702.
Bao S. Z., Miller D. J., Ma Z. M., Wohltmann M., Eng G., Ramanadham S., Moley K., and Turk J.-(2004). Male mice that do not express group VIA phospholipase A2 produce spermatozoa with impaired motility and have greatly reduced fertility. J.-Biol. Chem. 279:38194–38200.
Bassa B. V., Roh D. D., Vaziri N. D., Kirschenbaum M. A., and Kamanna V. S. (1999). Lysophosphatidylcholine activates mesangial cell PKC and MAP kinase by PLCγ-1 and tyrosine kinase-Ras pathways. Am. J.-Physiol. 277:F328–F337.
Bernoud N., Fenart L., Molière P., Dehouck M. P., Lagarde M., Cecchelli R., and Lecerf J.-(1999). Preferential transfer of 2-docosahexaenoyl-1-lysophosphatidylcholine through an in-vitro blood–brain barrier over unesterified docosahexaenoic acid. J.-Neurochem. 72:338–345.
Birgbauer E., Rao T. S., and Webb M. (2004). Lysolecithin induces demyelination in-vitro in a cerebellar slice culture system. J.-Neurosci. Res. 78:157–166.
Boggs K. P., Rock C. O., and Jackowski S. (1995). Lysophosphatidylcholine and 1-O-octadecyl-2-O-methyl-rac-glycero-3-phosphocholine inhibit the CDP-choline pathway of phosphatidylcholine synthesis at the CTP: phosphocholine cytidylyltransferase step. J.-Biol. Chem. 270:7757–7764.
Bruni A., Bigon E., Boarato E., Mietto L., Leon A., and Toffano G. (1982). Interaction between nerve growth factor and lysophosphatidylserine on rat peritoneal mast cells. FEBS Lett. 138:190–192.
Bruni A., Bigon E., Battistella A., Boarato E., Mietto L., and Toffano G. (1984). Lysophosphatidylserine as histamine releaser in mice and rats. Agents Actions 14:619–625.
Bruni A., Monastra G., Bellini F., and Toffano G. (1988). Autacoid properties of lysophosphatidylserine. Prog. Clin. Biol. Res. 282:165–179.
Caldwell R. A. and Baumgarten C. M. (1998). Plasmalogen-derived lysolipid induces a depolarizing cation current in rabbit ventricular myocytes. Circ. Res. 83:533–540.
Casado M. and Ascher P. (1998). Opposite modulation of NMDA receptors by lysophospholipids and arachidonic acid: common features with mechanosensitivity. J.-Physiol. 513(Pt 2):317–330.
Chaudhuri P., Colles S. M., Damron D. S., and Graham L. M. (2003). Lysophosphatidylcholine inhibits endothelial cell migration by increasing intracellular calcium and activating calpain. Arterioscler. Thromb. Vasc. Biol. 23:218–223.
Chernomordik L., Chanturiya A., Green J., and Zimmerberg J.-(1995). The hemifusion intermediate and its conversion to complete fusion: regulation by membrane composition. Biophys. J.-69:922–929.
Corr P. B., Yamada K. A., Creer M. H., Wu J., McHowat J., and Yan G. X. (1995). Amphipathic lipid metabolites and arrythmias during ischemia. In: Zipes D. P. and Jalife J.-(eds.), Cardiac Electrophysiology: From Cell to Bedside. W. B. Saunders, Philadelphia, pp.-182–203.
Cox D. A. and Cohen M. L. (1996). Lysophosphatidylcholine stimulates phospholipase D in human coronary endothelial cells: Role of PKC. Am. J.-Physiol. Heart Circ. Physiol. 271:H1706–H1710.
Degaonkar M. N., Khubchandhani M., Dhawan J.-K., Jayasundar R., and Jagannathan N. R. (2002). Sequential proton MRS study of brain metabolite changes monitored during a complete pathological cycle of demyelination and remyelination in a lysophosphatidyl choline (LPC)-induced experimental demyelinating lesion model. NMR Biomed. 15:293–300.
Degaonkar M. N., Raghunathan P., Jayasundar R., and Jagannathan N. R. (2005). Determination of relaxation characteristics during preacute stage of lysophosphatidyl choline-induced demyelinating lesion in rat brain: an animal model of multiple sclerosis. Magn. Reson. Imaging 23:69–73.
Durante W., Liao L., Peyton K. J., and Schafer A. I. (1997). Lysophosphatidylcholine regulates cationic amino acid transport and metabolism in vascular smooth muscle cells. Role in polyamine biosynthesis. J.-Biol. Chem. 272:30154–30159.
Falasca M., Silletta M. G., Carvelli A., Di Francesco A. L., Fusco A., Ramakrishna V., and-Corda D. (1995). Signalling pathways involved in the mitogenic action of lysophosphatidylinositol. Oncogene 10:2113–2124.
Falasca M., Iurisci C., Carvelli A., Sacchetti A., and Corda D. (1998). Release of the mitogen lysophosphatidylinositol from H-Ras-transformed fibroblasts; a possible mechanism of autocrine control of cell proliferation. Oncogene 16:2357–2365.
Fang X., Gibson S., Flowers M., Furui T., Bast R. C., Jr., and Mills G. B. (1997). Lysophosphatidylcholine stimulates activator protein 1 and the c-Jun N-terminal kinase activity. J.-Biol. Chem. 272:13683–13689.
Farooqui A. A. and Horrocks L. A. (2001). Plasmalogens: workhorse lipids of membranes in normal and injured neurons and glia. Neuroscientist 7:232–245.
Farooqui A. A. and Horrocks L. A. (2004a). Brain phospholipases A2: a perspective on the history. Prostaglandins Leukot. Essent. Fatty Acids 71:161–169.
Farooqui A. A. and Horrocks L. A. (2004b). Plasmalogens, platelet activating factor, and other ether lipids. In: Nicolaou A. and Kokotos G. (eds.), Bioactive Lipids. Oily Press, Bridgwater, England, pp.-107–134.
Farooqui A. A. and Horrocks L. A. (2006). Phospholipase A2-generated lipid mediators in brain: the good, the bad, and the ugly. Neuroscientist 12:245.
Farooqui A. A., Pendley C. E., II, Taylor W. A., and Horrocks L. A. (1985). Studies on diacylglycerol lipases and lysophospholipases of bovine brain. In: Horrocks L. A., Kanfer J.-N., and Porcellati G. (eds.), Phospholipids in the Nervous System, Vol. II: Physiological Role. Raven Press, New York, pp.-179–192.
Farooqui A. A., Yang H. C., Rosenberger T. A., and Horrocks L. A. (1997). Phospholipase A2 and its role in brain tissue. J.-Neurochem. 69:889–901.
Farooqui A. A., Horrocks L. A., and Farooqui T. (2000). Deacylation and reacylation of neural membrane glycerophospholipids. J.-Mol. Neurosci. 14:123–135.
Farooqui A. A., Horrocks L. A., and Farooqui T. (2006). Choline and ethanolamine glycerophospholipids. In: Tettamanti G. and Goracci G. (eds.), Handbook of Neurochemistry. Springer, New York.
Fink K. L. and Gross R. W. (1984). Modulation of canine myocardial sarcolemmal membrane fluidity by amphiphilic compounds. Circ. Res. 55:585–594.
Flemming P. K., Dedman A. M., Xu S. Z., Li J., Zeng F., Naylor J., Benham C. D., Bateson A. N., Muraki K., and Beech D. J.-(2006). Sensing of lysophospholipids by TRPC5 calcium channel. J.-Biol. Chem. 281:4977–4982.
Fuller N. and Rand R. P. (2001). The influence of lysolipids on the spontaneous curvature and bending elasticity of phospholipid membranes. Biophys. J.-81:243–254.
Garsetti D. E., Ozgur L. E., Steiner M. R., Egan R. W., and Clark M. A. (1992). Isolation and characterization of three lysophospholipases from the murine macrophage cell line WEHI 265.1. Biochim. Biophys. Acta Lipids Lipid Metab. 1165:229–238.
Goel D. P., Ford D. A., and Pierce G. N. (2003). Lysophospholipids do not directly modulate Na+–H+ exchange. Mol. Cell. Biochem. 251:3–7.
Gómez-Muñoz A., O’Brien L., Hundal R., and Steinbrecher U. P. (1999). Lysophosphatidylcholine stimulates phospholipase D activity in mouse peritoneal macrophages. J.-Lipid Res. 40:988–993.
Han X. and Gross R. W. (1991). Proton nuclear magnetic resonance studies on the molecular dynamics of plasmenylcholine/cholesterol and phosphatidylcholine/cholest rol bilayers. Biochim. Biophys. Acta Biomembr. 1063:129–136.
Han X. L., Holtzman D. M., and McKeel D. W., Jr. (2001). Plasmalogen deficiency in early Alzheimer’s disease subjects and in animal models: molecular characterization using electrospray ionization mass spectrometry. J.-Neurochem. 77:1168–1180.
Horigome K., Tamori-Natori Y., Inoue K., and Nojima S. (1986). Effect of serine phospholipid structure on the enhancement of concanavalin A-induced degranulation in rat mast cells. J.-Biochem. (Tokyo) 100:571–579.
Horigome K., Hayakawa M., Inoue K., and Nojima S. (1987). Purification and characterization of phospholipase A2 released from rat platelets. J.-Biochem. (Tokyo) 101:625–631.
Horigome K., Pryor J.-C., Bullock E. D., and Johnson E. M., Jr. (1993). Mediator release from mast cells by nerve growth factor. neurotrophin specificity and receptor mediation. J.-Biol. Chem. 268:14881–14887.
Hosono H., Aoki J., Nagai Y., Bandoh K., Ishida M., Taguchi R., Arai H., and Inoue K. (2001). Phosphatidylserine-specific phospholipase A1 stimulates histamine release from rat peritoneal mast cells through production of 2-acyl-1-lysophosphatidylserine. J.-Biol. Chem. 276:29664–29670.
Ikeda Y., Fukuoka S., and Kito M. (1997). Increase in lysophosphatidylethanolamine in the cell membrane upon the regulated exocytosis of pancreatic acinar AR42J cells. Biosci. Biotechnol. Biochem. 61:207–209.
Ikeuchi Y., Nishizaki T., and Matsuoka T. (1995). Lysophosphatidylcholine inhibits NMDA-induced currents by a mechanism independent of phospholipase A2-mediated protein kinase C activation in hippocampal glial cells. Biochem. Biophys. Res. Commun. 217:811–816.
Ikeuchi Y., Nishizaki T., Matsuoka T., and Sumikawa K. (1997). Long-lasting enhancement of ACh receptor currents by lysophospholipids. Brain Res. Mol. Brain Res. 45:317–320.
Inoue K., Kobayashi T., and Kudo I. (1989). Function and metabolism of lysophosphatidylserine in rat mast cell activation. In: Bazan N. G., Horrocks L. A., and Toffano G. (eds.), Phospholipids in the Nervous System, Biochemical and Molecular Pathology. Liviana Press, Padova, pp.-225–231.
Iwata H., Ohta A., and Baba A. (1986). Stimulatory effect of veratridine on lysophosphatidylethanolamine formation in rat brain synaptosomes. Jpn J.-Pharmacol. 41:293–297.
Ji R. R., Kohno T., Moore K. A., and Woolf C. J.-(2003). Central sensitization and LTP: do pain and memory share similar mechanisms? Trends Neurosci. 26:696–705.
Jurkowitz M. S., Horrocks L. A., and Litsky M. L. (1999). Identification and characterization of alkenyl hydrolase (lysoplasmalogenase) in microsomes and identification of a plasmalogen-active phospholipase A2 in cytosol of small intestinal epithelium. Biochim. Biophys. Acta Lipids Lipid Metab. 1437:142–156.
Jurkowitz-Alexander M., Ebata H., Mills J.-S., Murphy E. J., and Horrocks L. A. (1989). Solubilization, purification, and characterization of lysoplasmalogen alkenylhydrolase (lysoplasmalogenase) from rat liver microsomes. Biochim. Biophys. Acta 1002:203–212.
Kern R., Joseleau-Petit D., Chattopadhyay M. K., and Richarme G. (2001). Chaperone-like properties of lysophospholipids. Biochem. Biophys. Res. Commun. 289:1268–1274.
Kobayashi T., Kishimoto M., and Okuyama H. (1996). Phospholipases involved in lysophosphatidylinositol metabolism in rat brain. J.-Lipid Mediat. Cell Signal. 14:33–37.
Kume N. and Gimbrone M. A., Jr. (1994). Lysophosphatidylcholine transcriptionally induces growth factor gene expression in cultured human endothelial cells. J.-Clin. Invest. 93:907–911.
Kume N., Cybulsky M. I., and Gimbrone M. A., Jr. (1992). Lysophosphatidylcholine, a component of atherogenic lipoproteins, induces mononuclear leukocyte adhesion molecules in cultured human and rabbit arterial endothelial cells. J.-Clin. Invest. 90:1138–1144.
Lambert I. H. and Falktoft B. (2000). Lysophosphatidylcholine induces taurine release from HeLa cells. J.-Membr. Biol. 176:175–185.
Lee T. C. (1998). Biosynthesis and possible biological functions of plasmalogens. Biochim. Biophys. Acta Lipids Lipid Metab. 1394:129–145.
Lee E. S. Y., Chen H. T., Shepherd K. R., Lamango N. S., Soliman K. F. A., and Charlton C. G. (2004). Inhibitory effects of lysophosphatidylcholine on the dopaminergic system. Neurochem. Res. 29:1333–1342.
Lee E. S. Y., Soliman K. F. A., and Charlton C. G. (2005). Lysophosphatidylcholine decreases locomotor activities and dopamine turnover rate in rats. Neurotoxicology 26:27–38.
Légrádi A., Chitu V., Szukacsov V., Fajka-Boja R., Szücs K. S., and Monostori E. (2004). Lysophosphatidylcholine is a regulator of tyrosine kinase activity and intracellular Ca2+ level in Jurkat T cell line. Immunol. Lett. 91:17–21.
Leitinger N. (2005). Oxidized phospholipids as triggers of inflammation in atherosclerosis. Mol. Nutr. Food Res. 49:1063–1071.
Leslie C. C. (1991). Kinetic properties of a high molecular mass arachidonoyl-hydrolyzing phospholipase A2 that exhibits lysophospholipase activity. J.-Biol. Chem. 266:11366–11371.
Lesnefsky E. J., Stoll M. S. K., Minkler P. E., and Hoppel C. L. (2000). Separation and quantitation of phospholipids and lysophospholipids by high-performance liquid chromatography. Anal. Biochem. 285:246–254.
Lourenssen S. and Blennerhassett M. G. (1998). Lysophosphatidylserine potentiates nerve growth factor-induced differentiation of PC12 cells. Neurosci. Lett. 248:77–80.
Lovas G., Palkovits M., and Komoly S. (2000). Increased c-Jun expression in neurons affected by lysolecithin-induced demyelination in rats. Neurosci. Lett. 292:71–74.
Lundbaek J.-A. and Andersen O. S. (1994). Lysophospholipids modulate channel function by altering the mechanical properties of lipid bilayers. J.-Gen. Physiol. 104:645–673.
Maingret F., Patel A. J., Lesage F., Lazdunski M., and Honoré E. (2000). Lysophospholipids open the two-pore domain mechano-gated K+ channels TREK-1 and TRAAK. J.-Biol. Chem. 275:10128–10133.
Mazurek N., Weskamp G., Erne P., and Otten U. (1986). Nerve growth factor induces mast cell degranulation without changing intracellular calcium levels. FEBS Lett. 198:315–320.
Mietto L., Boarato E., Toffano G., and Bruni A. (1987). Lysophosphatidylserine-dependent interaction between rat leukocytes and mast cells. Biochim. Biophys. Acta 930:145–153.
Muir L. V., Born E., Mathur S. N., and Field F. J.-(1996). Lysophosphatidylcholine increases 3-Hydroxy-3-methylglutaryl-coenzyme A reductase gene expression in CaCo-2 cells. Gastroenterology 110:1068–1076.
Murugesan G., Rani M. R. S., Gerber C. E., Mukhopadhyay C., Ransohoff R. M., Chisolm G. M., and Kottke-Marchant K. (2003). Lysophosphatidylcholine regulates human microvascular endothelial cell expression of chemokines. J.-Mol. Cell Cardiol. 35:1375–1384.
Nagai Y., Aoki J., Sato T., Amano R., Matsuda Y., Arai H., and Inoue K. (1999). An alternative splicing form of phosphatidylserine-specific phospholipase A1 that exhibits lysophosphatidylserine-specific lysophospholipase activity in humans. J.-Biol. Chem. 274:11053–11059.
Nakano T., Raines E. W., Abraham J.-A., Klagsbrun M., and Ross R. (1994). Lysophosphatidylcholine upregulates the level of heparin-binding epidermal growth factor-like growth factor mRNA in human monocytes. Proc. Natl Acad. Sci. USA 91:1069–1073.
Oishi K., Raynor R. L., Charp P. A., and Kuo J.-F. (1988). Regulation of protein kinase C by lysophospholipids. Potential role in signal transduction. J.-Biol. Chem. 263:6865–6871.
Ousman S. S. and David S. (2000). Lysophosphatidylcholine induces rapid recruitment and activation of macrophages in the adult mouse spinal cord. Glia 30:92–104.
Park K. S., Lee H. Y., Kim M. K., Shin E. H., and Bae Y. S. (2005). Lysophosphatidylserine stimulates leukemic cells but not normal leukocytes. Biochem. Biophys. Res. Commun. 333:353–358.
Park K. S., Lee H. Y., Kim M. K., Shin E. H., Jo S. H., Kim S. D., Im D. S., and Bae Y. S. (2006). Lysophosphatidylserine stimulates L2071 mouse fibroblast chemotactic migration via a process involving pertussis toxin-sensitive trimeric G-proteins. Mol. Pharmacol. 69:1066–1073.
Pete M. J.-and Exton J.-H. (1996). Purification of a lysophospholipase from bovine brain that selectively deacylates arachidonoyl-substituted lysophosphatidylcholine. J.-Biol. Chem. 271:18114–18121.
Poole A. R., Howell J.-I., and Lucy J.-A. (1970). Lysolecithin and cell fusion. Nature 227:810–814.
Rikitake Y., Hirata K., Kawashima S., Takeuchi S., Shimokawa Y., Kojima Y., Inoue N., and Yokoyama M. (2001). Signaling mechanism underlying COX-2 induction by lysophosphatidylcholine. Biochem. Biophys. Res. Commun. 281:1291–1297.
Ross B. M. and Kish S. J.-(1994). Characterization of lysophospholipid metabolizing enzymes in human brain. J.-Neurochem. 63:1839–1848.
Ryu S. B. and Palta J.-P. (2000). Specific inhibition of rat brain phospholipase D by lysophospholipids. J.-Lipid Res. 41:940–944.
Sakai M., Miyazaki A., Hakamata H., Sasaki T., Yui S., Yamazaki M., Shichiri M., and Horiuchi S. (1994). Lysophosphatidylcholine plays an essential role in the mitogenic effect of oxidized low density lipoprotein on murine macrophages. J.-Biol. Chem. 269:31430–31435.
Schilling T., Lehmann F., Ruckert B., and Eder C. (2004). Physiological mechanisms of lysophosphatidylcholine-induced de-ramification of murine microglia. J.-Physiol. (Lond.) 557:105–120.
Seebeck J., Westenberger K., Elgeti T., Ziegler A., and Schutze S. (2001). The exocytotic signaling pathway induced by nerve growth factor in the presence of lyso-phosphatidylserine in rat peritoneal mast cells involves a type D phospholipase. Regul. Pept. 102:93–99.
Soga T., Ohishi T., Matsui T., Saito T., Matsumoto M., Takasaki J., Matsumoto S., Kamohara M., Hiyama H., Yoshida S., Momose K., Ueda Y., Matsushime H., Kobori M., and Furuichi K. (2005). Lysophosphatidylcholine enhances glucose-dependent insulin secretion via an orphan G-protein-coupled receptor. Biochem. Biophys. Res. Commun. 326:744–751.
Sun G. Y. and MacQuarrie R. A. (1989). Deacylation–reacylation of arachidonoyl groups in cerebral phospholipids. Ann. NY Acad. Sci. 559:37–55.
Tsutsumi T., Kobayashi T., Ueda H., Yamauchi E., Watanabe S., and Okuyama H. (1994). Lysophosphoinositide-specific phospholipase C in rat brain synaptic plasma membranes. Neurochem. Res. 19:399–406.
Ueda H., Kobayashi T., Kishimoto M., Tsutsumi T., and Okuyama H. (1993). A possible pathway of phosphoinositide metabolism through EDTA-insensitive phospholipase A1 followed by lysophosphoinositide-specific phospholipase C in rat brain. J.-Neurochem. 61:1874–1881.
Vahidi W. H., Ong W. Y., Farooqui A. A., and Yeo J.-F. (2006). Pronociceptive effect of central nervous lysophospholipids in a mouse model of orofacial pain. Exp. Brain Res. (in press).
Vogel S. S., Leikina E. A., and Chernomordik L. V. (1993). Lysophosphatidylcholine reversibly arrests exocytosis and viral fusion at a stage between triggering and membrane merger. J.-Biol. Chem. 268:25764–25768.
Wang A. and Dennis E. A. (1999). Mammalian lysophospholipases. Biochim. Biophys. Acta 1439:1–16.
Wang A., Yang H. C., Friedman P., Johnson C. A., and Dennis E. A. (1999). A specific human lysophospholipase: cDNA cloning, tissue distribution and kinetic characterization. Biochim. Biophys. Acta 1437:157–169.
Weltzien H. U. (1979). Cytolytic and membrane-perturbing properties of lysophosphatidylcholine. Biochim. Biophys. Acta 559:259–287.
Williams S. D. and Ford D. A. (1997). Activation of myocardial cAMP-dependent protein kinase by lysoplasmenylcholine. FEBS Lett. 420:33–38.
Yamashita A., Watanabe M., Sato K., Miyashita T., Nagatsuka T., Kondo H., Kawagishi N., Nakanishi H., Kamata R., Sugiura T., and Waku K. (2003). Reverse reaction of lysophosphatidylinositol acyltransferase –– functional reconstitution of coenzyme A-dependent transacylation system. J.-Biol. Chem. 278:30382–30393.
Yeo J.-F., Ong W. Y., Ling S. F., and Farooqui A. A. (2004). Intracerebroventricular injection of phospholipases A2 inhibitors modulates allodynia after facial carrageenan injection in mice. Pain 112:148–155.
Yuan Y., Schoenwaelder S. M., Salem H. H., and Jackson S. P. (1996). The bioactive phospholipid, lysophosphatidylcholine, induces cellular effects via G-protein-dependent activation of adenylyl cyclase. J.-Biol. Chem. 271:27090–27098.
Zembowicz A., Jones S. L., and Wu K. K. (1995). Induction of cyclooxygenase-2 in human umbilical vein endothelial cells by lysophosphatidylcholine. J.-Clin. Invest. 96:1688–1692.
Zhu Y., Lin J.-H. C., Liao H. L., Verna L., and Stemerman M. B. (1997). Activation of ICAM-1 promoter by lysophosphatidylcholine: possible involvement of protein tyrosine kinases. Biochim. Biophys. Acta Lipids Lipid Metab. 1345:93–98.
Zhu K., Baudhuin L. M., Hong G., Williams F. S., Cristina K. L., Kabarowski J.-H., Witte O. N., and Xu Y. (2001). Sphingosylphosphorylcholine and lysophosphatidylcholine are ligands for the G protein-coupled receptor GPR4. J.-Biol. Chem. 276:41325–41335.
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(2007). Lyso-Glycerophospholipids. In: Glycerophospholipids in the Brain. Springer, New York, NY. https://doi.org/10.1007/978-0-387-49931-4_8
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