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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 593))

Abstract

DNA microarray technology has become a powerful tool in the arsenal of the molecular biologist. Capitalizing on high precision robotics and the wealth of DNA sequences annotated from the genomes of a large number of organisms, the manufacture of microarrays is now possible for the average academic laboratory with the funds and motivation. Microarray production requires attention to both biological and physical resources, including DNA libraries, robotics, and qualified personnel. While the fabrication of microarrays is a very labor-intensive process, production of quality microarrays individually tailored on a project-by-project basis will help researchers shed light on future scientific questions.

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References

  1. Schena M et al. Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 1995; 270:467–470.

    Article  PubMed  CAS  Google Scholar 

  2. Schena M et al. Parallel human genome analysis: Microarray-based expression monitoring of 1000 genes. Proc Natl Acad Sci USA 1996; 93:10614–10619.

    Article  PubMed  CAS  Google Scholar 

  3. Hughes TR et al. Expression profiling using microarrays fabricated by an ink-jet oligonucleotide synthesizer. Nat Biotechnol 2001; 19:342–347.

    Article  PubMed  CAS  Google Scholar 

  4. Kane MD et al. Assessment of the sensitivity and specificity of oligonucleotide (50 mer) microarrays. Nucleic Acid Res 2000; 28:4552–4557.

    Article  PubMed  CAS  Google Scholar 

  5. Lausted C et al. POSaM: A fast, flexible, open-source, inkjet oligonucleotide synthesizer and microarrayer. Genome Biology 2004; 5(8):R58.

    Article  PubMed  Google Scholar 

  6. Diehl F et al. Manufacturing DNA microarrays of high spot homogeneity and reduced background signal. Nucl Acids Res 2001; 29(7):E38.

    Article  PubMed  CAS  Google Scholar 

  7. Wrobel G et al. Optimization of high-density cDNA-microarray protocols by’ design of experiments. Nucleic Acids Res 2003; 31:67.

    Article  Google Scholar 

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Correspondence to David W. Petersen .

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© 2007 Landes Bioscience and Springer Science+Business Media

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Petersen, D.W., Kawasaki, E.S. (2007). Manufacturing of Microarrays. In: Mocellin, S. (eds) Microarray Technology and Cancer Gene Profiling. Advances in Experimental Medicine and Biology, vol 593. Springer, New York, NY. https://doi.org/10.1007/978-0-387-39978-2_1

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