Abstract
Kinetics of conjugate formation between cytotoxic lymphocytes and tumour cells is described using the enzyme kinetics model and adapted for the evaluation of in vitro measurements by double fluorescence flow cytometry. The parameters of the model were estimated for two effector-target systems. A previously reported model of the first order kinetics is shown to be an approximation for the presented model. A parameter, derived from the model and called the strength of binding, is proposed for a simple and objective evaluation of the experiments.
Chapter PDF
Similar content being viewed by others
References
Garcia-Penarrubia, P., Koster, F.T., Bankhurst, A.D. (1989a) Model for population
distributions of lymphocyte-target cell conjugates. J Theor Biol, 138, 77–92. Garcia-Penarrubia, P., Koster, F.T., Bankhurst, A.D. (1989b) The maximum conjugate
frequency (am,x) characterizes killer cell populations. JlmmunolMethods,118 199–208. Murray, J.D. (1989) Mathematical Biology. Springer, Berlin.
Palucka, A.K., Waniewski, J. and Porwit, A. (1995) Strength of binding between leukemic blasts and cytotoxic lymphocytes. Cytometry, submitted.
Perez, P., Bluestone, J.A., Stephany, D.A. and Segal DM. (1985) Quantitative measurements of the specificity and kinetics of conjugate formation between cloned cytotoxic T lymphocyte and splenic target cells by dual parameters flow cytometry. J Immunol, 134, 478–85.
Segal, D.M. and Stephany, D.A. (1984) The measurement of specific cell: cell interactions by dual-parameter flow cytometry. Cytometry, 5, 169–81.
Thoma, J.A., Touton, M.H. and Clark, W.R. (1978) Interpretation of “Cr -release data: a kinetic analysis. Jlmmunol, 120, 991–7.
Waniewski, J., Palucka, A.K. and Porwit, A. (1993) Kinetic analysis of cytotoxic lymphocyte - target cell interaction as quantified by dual parameter flow cytometry. Cytometry, 14, 393400.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Waniewski, J., Palucka, A.K., Porwit, A. (1996). Mathematical modelling of conjugate formation by cytotoxic lymphocytes and tumour cells. In: Doležal, J., Fidler, J. (eds) System Modelling and Optimization. IFIP — The International Federation for Information Processing. Springer, Boston, MA. https://doi.org/10.1007/978-0-387-34897-1_39
Download citation
DOI: https://doi.org/10.1007/978-0-387-34897-1_39
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-6671-4
Online ISBN: 978-0-387-34897-1
eBook Packages: Springer Book Archive