Germinal Center-Derived B Cell Memory
B cell memory is characterized by persistent levels of Ag-specific serum antibody (Ab) following immunization and the ability to rapidly produce Ab upon secondary Ag exposure1. During primary immune responses in mammals, B cell activation occurs within or at the border of T cell-rich periarteriolar lymphoid sheaths following cognate interaction with CD4 TH cells2,3. This interaction induces B lymphocytes to differentiate into either foci of antibody-forming cells (AFC) found in red pulp and follicular borders or germinal center (GC) B cells located within secondary follicles2, 3, 4. The primary foci response in mice is relatively short lived (5–10 days); in contrast, the GC response is a more sustained program of cellular differentiation in which extensive B cell proliferation, somatic hypermutation of IgV gene segments, and memory cell selection occurs4, 5, 6, 7, 8. While GC B cells are readily identified using cell surface attributes, no such markers have been identified on murine memory B cells. The inability to reliably identify memory B cells has hampered the study of memory B cell development and differentiation.
KeywordsCell Memory Germinal Center Adoptive Transfer ELISPOT Assay Primary Immune Response
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