5. Conclusions
Taurine-induced changes in expression profiling of HepG2 cells were assessed using a cDNA microarray technology and confirmed by real time RT-PCR analyses. Among known genes regulated by taurine, 87 genes were up-regulated and 206 genes downregulated more than two-fold. Among these 293 taurine-responsive genes, 30 genes were implicated in the processes of carbohydrate, lipid or protein/amino acid metabolism. Taurine-induced up-regulation of UDP-glucose pyrophosphorylase 2 and down-regulation of protein phosphatase 1 regulatory subunit 3A expressions lead to a hypothesis that taurine enhances glycogen synthesis in the liver. Taurine-induced down-regulation of hepatic pyruvate dehydrogenase kinase isoenzyme 4 expression suggests that taurine increases pyruvate dehydrogenase complex activity, generating acetyl-CoA for further oxidation of three-carbon compounds via TCA cycle. Our results of cDNA-microarray and real-time RT-PCR also indicated that taurine up-regulated expressions of four enzymes involved in branched-chain amino acid catabolism (branched-chain amino acid aminotransferases 2; mitochondrial, branched-chain aminotransferase 1, cytosolic; branched-chain keto acid dehydrogenase; 3-hydroxy-3-methylglutaryl-CoA lyase). Our microarray results of hepatic gene expression profiling would provide further insights into molecular actions of taurine in metabolic regulations.
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Park, SH., Lee, H., Park, K.K., Kim, H.W., Lee, D.H., Park, T. (2006). Taurine-Induced Changes in Transcription Profiling of Metabolism-Related Genes in Human Hepatoma Cells HepG2. In: Oja, S.S., Saransaari, P. (eds) Taurine 6. Advances in Experimental Medicine and Biology, vol 583. Springer, Boston, MA . https://doi.org/10.1007/978-0-387-33504-9_12
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