Effects of Mutagenesis of Murine Hepatitis Virus NSP1 and NSP14 on Replication in Culture

  • Lance D. Eckerle
  • Sarah M. Brockway
  • Steven M. Sperry
  • Xiaotao Lu
  • Mark R. Denison
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 581)


Infectious Virus Mouse Hepatitis Virus Electroporated Cell Murine Hepatitis Virus Viable Mutant 
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  1. 1.
    Schiller, J. J., A. Kanjanahaluethai, and S. C. Baker, 1998, Processing of the coronavirus MHV-JHM polymerase polyprotein: identification of precursors and proteolytic products spanning 400 kilodaltons of ORF1a. Virology 242:288-302.CrossRefPubMedGoogle Scholar
  2. 2.
    Snijder, E. J., P. J. Bredenbeek, J. C. Dobbe, V. Thiel, J. Ziebuhr, L. L. Poon, Y. Guan, M. Rozanov, W. J. Spaan, and A. E. Gorbalenya, 2003, Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage. J Mol Biol 331:991-1004.CrossRefPubMedGoogle Scholar
  3. 3.
    Yount, B., M. R. Denison, S. R. Weiss, and R. S. Baric, 2002, Systematic assembly of a full-length infectious cDNA of mouse hepatitis virus strain A59. J Virol 76:11065-11078.Google Scholar
  4. 4.
    Sperry, S. M., L. Kazi, R. L. Graham, R. S. Baric, S. R. Weiss, and M. R. Denison, 2005, Single-amino-acid substitutions in open reading frame (ORF) 1b-nsp14 and ORF 2a proteins of the coronavirus mouse hepatitis virus are attenuating in mice. J Virol 79:3391-3400.CrossRefPubMedGoogle Scholar
  5. 5.
    Brockway, S. M., Lu, X. T., Peters, T. R., Dermody, T. S., and Denison, M. R. 2004. Intracellular localization and protein interactions of the gene 1 protein p28 during mouse hepatitis virus replication. J Virol 78:11551-11562.CrossRefPubMedGoogle Scholar
  6. 6.
    Denison, M.R., Spaan, J.M., van der Meer, Y., Gibson, C.A., Sims, A.C., Prentice, E., and Lu, X.T, 1999, The putative helicase of the coronavirus mouse hepatitis virus is processed from the replicase gene polyprotein and localizes in complexes that are active in viral RNA synthesis. J Virol 73:6862-6871.PubMedGoogle Scholar
  7. 7.
    Ziebuhr, J., E. J. Snijder, and A. E. Gorbalenya, 2000, Virus-encoded proteinases and proteolytic processing in the Nidovirales. J Gen Virol 81:853-879.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2006

Authors and Affiliations

  • Lance D. Eckerle
    • 1
  • Sarah M. Brockway
    • 2
  • Steven M. Sperry
    • 3
  • Xiaotao Lu
    • 4
  • Mark R. Denison
    • 5
  1. 1.Vanderbilt University Medical CenterNashvilleUSA
  2. 2.Vanderbilt University Medical CenterNashvilleUSA
  3. 3.Vanderbilt University Medical CenterNashvilleUSA
  4. 4.Vanderbilt University Medical CenterNashvilleUSA
  5. 5.Vanderbilt University Medical CenterNashvilleUSA

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