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Plastic Microfluidic Devices for DNA and Protein Analyses

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Book cover BioMEMS and Biomedical Nanotechnology

Abstract

Microfluidics and integrated microsystems are the current focus of unusually intense interest and activity on the part of academia, industry, and governmental agencies, an assertion substantiated by the solid attendance at the 2003 International Conference on Micro Total Analysis Systems (µTAS) [45] and by the recent publication of various books on this topic [46], both at a time when the economy in general, and the technology sector in particular, are at relative low points. Significant advances have been realized since the concept of µTAS was developed more than a decade ago as a means to enhance versatility and functionality relative to discrete chemical sensor devices [41]. Functional examples of the µTAS concept include on-chip PCR (polymerase chain reaction) [28, 44, 72], DNA analysis and sequencing [14, 58, 76], immunoassays [10, 19, 57], protein separations [23, 48, 64], and intra- and inter-cellular analysis [29, 53, 63]. Advantages of these µ-TAS over bench-top instruments include low reagent consumption, small sample volumes, high separation efficiencies, short reaction times, ease of automation, and potential for massproduction with low cost [41].

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Author information

Authors and Affiliations

  1. Department of Mechanical and Aerospace Engineering, Department of Biomedical Engineering, and McKnight Brain Institute, University of Florida, PO Box 116250, Gainesville, FL, 32611-6250

    Z. Hugh Fan

  2. NASA Ames Research Center, Mountain View, CA, 94035

    Antonio J. Ricco

  3. Dept. of Electrical Engineering, Stanford University, Stanford, CA, 94305

    Antonio J. Ricco

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  1. Z. Hugh Fan
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Editor information

Editors and Affiliations

  1. Department of Biomedical Engineering, University of Texas Health Science Center, Houston, TX

    Mauro Ferrari Ph.D. (Editor-in-Chief, Professor, Brown Institute of Molecular Medicine Chairman, Professor of Experimental Therapeutics, Professor of Bioengineering, Professor of Biochemistry and Molecular Biology, President) (Editor-in-Chief, Professor, Brown Institute of Molecular Medicine Chairman, Professor of Experimental Therapeutics, Professor of Bioengineering, Professor of Biochemistry and Molecular Biology, President)

  2. Anderson Cancer Center, University of Texas M.D., Houston, TX

    Mauro Ferrari Ph.D. (Editor-in-Chief, Professor, Brown Institute of Molecular Medicine Chairman, Professor of Experimental Therapeutics, Professor of Bioengineering, Professor of Biochemistry and Molecular Biology, President) (Editor-in-Chief, Professor, Brown Institute of Molecular Medicine Chairman, Professor of Experimental Therapeutics, Professor of Bioengineering, Professor of Biochemistry and Molecular Biology, President)

  3. Rice University, Houston, TX

    Mauro Ferrari Ph.D. (Editor-in-Chief, Professor, Brown Institute of Molecular Medicine Chairman, Professor of Experimental Therapeutics, Professor of Bioengineering, Professor of Biochemistry and Molecular Biology, President) (Editor-in-Chief, Professor, Brown Institute of Molecular Medicine Chairman, Professor of Experimental Therapeutics, Professor of Bioengineering, Professor of Biochemistry and Molecular Biology, President)

  4. University of Texas Medical Branch, Galveston, TX

    Mauro Ferrari Ph.D. (Editor-in-Chief, Professor, Brown Institute of Molecular Medicine Chairman, Professor of Experimental Therapeutics, Professor of Bioengineering, Professor of Biochemistry and Molecular Biology, President) (Editor-in-Chief, Professor, Brown Institute of Molecular Medicine Chairman, Professor of Experimental Therapeutics, Professor of Bioengineering, Professor of Biochemistry and Molecular Biology, President)

  5. Texas Alliance for NanoHealth, Houston, TX

    Mauro Ferrari Ph.D. (Editor-in-Chief, Professor, Brown Institute of Molecular Medicine Chairman, Professor of Experimental Therapeutics, Professor of Bioengineering, Professor of Biochemistry and Molecular Biology, President) (Editor-in-Chief, Professor, Brown Institute of Molecular Medicine Chairman, Professor of Experimental Therapeutics, Professor of Bioengineering, Professor of Biochemistry and Molecular Biology, President)

  6. Dept. of Electrical Engineering, University of California Riverside, Riverside, California, USA

    Mihrimah Ozkan

  7. Dept. of Bioengineering, University of California, San Diego La Jolla, California, USA

    Michael J. Heller

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Fan, Z.H., Ricco, A.J. (2006). Plastic Microfluidic Devices for DNA and Protein Analyses. In: Ferrari, M., Ozkan, M., Heller, M.J. (eds) BioMEMS and Biomedical Nanotechnology. Springer, Boston, MA. https://doi.org/10.1007/978-0-387-25843-0_9

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