Abstract
Microelectronic arrays utilizing electric field transport have been developed for DNA diagnostics (including infectious and genetic disease and cancer detection), for short tandem repeat (STR) forensics analysis, and for gene expression applications. In addition to these bioresearch and clinical diagnostic applications, such devices also have the potential to carry out the assisted assembly of a wide variety of molecular scale, nanoscale and microscale components into higher order structures. These microelectronic array devices are able to produce defined electric fields on their surfaces that allow molecules and other entities with high fidelity recognition properties to be transported to or from any site on the surface of the array. Such devices can utilize either DC electric fields which cause movement of entities by their relative charge, or AC electric fields which allow entities to be selectively positioned by their dielectric properties. An almost unlimited variety of molecules and nanocomponents can be utilized with these devices, including: DNA, DNA constructs with fluorescent, photonic or electronic transfer properties, RNA, RNA constructs, amino acids, peptides, proteins (antibodies, enzymes), nanoparticles (quantum dots, carbon nanotubes, nanowires), cells and even micron scale semiconductor components. Thus, electric field devices can be used for developing a unique highly parallel “Pick & Place” fabrication process by which a variety of heterogeneous molecules, nanocomponents and micron sized objects with intrinsic self-assembly properties can be organized into higher order 2D and 3D structures and devices. The process represents a unique synergy of combining the best aspects of a “top-down” process with a “bottom-up” process. Finally, integration of optical tweezers for manipulation of live cells and microspheres in a similar microarray setup is demonstrated for the applications of biological delivery and invasive manipulation of these species.
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References
Small Wonders, Endless Frontiers: Review of the Nanotional Nanotechnology Initiative, National Research Council, 2002.
M.P. Hughes (ed.). Nanoelectromechanics in Engineering and Biology. CRC Press, Boca Raton, FL, 2003.
Goddard, Brenner, Lyashevski, and Lafrate (ed.). Handbook of Nanoscience, Engineering and Technology. CRC Press, Boca Raton, FL, 2003.
V. Balzani, M. Venturi, and A. Credi. Molecular Devices and Mechanics—Journey into the Nanoworld. Wiley-VCH, KGaA Weinheim, 2003.
R. Bashir. Biological mediated assembly of artificial nanostructures and Microtructures. In Goddard, Brenner, Lyashevski and Lafrate (eds.), Handbook of Nanoscience, Engineering and Technology. CRC Press, Chapter 15, pp. 15-1 to 15-31, 2003.
M.J. Heller and R.H. Tullis. Nanotechnology, 2:165–171, 1991.
Daniel M. Hartmann, David Schwartz, Gene Tu, Mike Heller, Sadik C. Esener. Selective DNA attachment of particles to substrates. J. Mat. Res., 17(2):473–478, 2002.
M.J. Heller. An active microelectronics device for multiplex DNA analysis. IEEE Eng. Med. Biol., 15:100–103, 1996.
R.G. Sosnowski, E. Tu, W.F. Butler, J.P. O’Connell, and M.J. Heller. Rapid determination of single base mismatch in DNA hybrids by direct electric field control. Proc. Nat. Acad. Sci. USA, 94:1119–1123, 1997.
C.F. Edman, D.E. Raymond, D.J. Wu, E. Tu, R.G. Sosnowski, W.F. Butler, M. Nerenberg, and M.J. Heller. Electric field directed nucleic acid hybridization on microchips. Nucleic Acids Res., 25(24):4907–4914, 1997.
M.J. Heller. An integrated microelectronic hybridization system for genomic research and diagnostic applications. In D.J. Harrison and A. van den Berg (eds.), Micro Total Analysis Systems 98, Kluwer Academic Publishers, pp. 221–224, 1998.
M.J. Heller, E. Tu, A. Holmsen, R.G. Sosnowski, and J.P. O’Connell. Active Microelectronic Arrays forDNA Hybridization Analysis. In M. Schena (ed.), DNA Microarrays: A Practical Approach, Oxford University Press, pp. 167–185, 1999.
M.J. Heller, A.H. Forster, E. Tu. Active microelectronic chip devices which utilize controlled electrophoretic fields for multiplex DNA hybridization and genomic applications. Electrophoresis, 21:157–64, 2000.
C. Gurtner, E. Tu, N. Jamshidi, R. Haigis, T. Onofrey, C.F. Edman, R. Sosnowski, B. Wallace, and M.J. Heller. Microelectronic array devices and techniques for electric field enhanced DNA hybridization in lowconductance buffers, Electrophoresis, 23:1543–1550, 2002.
M.J. Heller. DNA microarray technology: devices, systems and applications, Ann. Rev. Biomed. Eng., 4:129–53, 2002.
M.J. Heller, E. Tu, R. Martinsons, R.R. Anderson, C. Gurtner, A. Forster, and R. Sosnowski. Active microelectronic array systems for DNA hybridization, genotyping, pharmacogenomics and nanofabrication applications. In Heller and Guttman (eds.), Integrated Microfabricated Devices, Marcel Dekker, Chap. 10, pp. 223–270, 2002.
S.K. Kassengne, H. Reese, D. Hodko, J.M. Yang, K. Sarkar, D.E. Swanson P. Raymond, M.J. Heller, and M.J. Madou. Numerical modeling of transport and accumulation of DNA on electronically active biochips, Sens. Actu. B, 94:81–98, 2003.
S.C. Esener, D. Hartmann, M.J. Heller, and J.M. Cable. DNA Assisted Micro-Assembly: A Heterogeneous Integration Technology For Optoelectronics, Proc. SPIE Critical Reviews of Optical Science and Technology, Heterogeneous Integration, Ed. A. Hussain, CR70, Chapter 7, January 1998.
C. Gurtner, C.F. Edman, R.E. Formosa, and M.J. Heller. Photoelectrophoretic Transport and Hybridization of DNA on Unpatterned Silicon Substrates. J. Am. Chem. Soc., 122(36):8589–8594, 2000.
Y. Huang, K.L. Ewalt, M. Tirado, R. Haigis, A. Forster, D. Ackley, M.J. Heller, J.P. O’Connell, and M. Krihak. Electric manipulation of bioparticles and macromolecules on microfabricated electrodes. Anal. Chem., 73:1549–1559, 2001.
C.F. Edman, C. Gurtner, R.E. Formosa, J.J. Coleman, and M.J. Heller. Electric-field-directed pick-and-place assembly. HDI, (3)10:30–35, 2000.
C.F. Edman, R.B. Swint, C. Gurthner, R.E. Formosa, S.D. Roh, K.E. Lee, P.D. Swanson, D.E. Ackley, J.J. Colman, and M.J. Heller. Electric field directed assembly of an InGaAs LED onto silicon circuitry. IEEE Photonics Tech. Lett., 12(9):1198–1200, 2000.
US # 6,569,382 Methods and Apparatus for the Electronic Homogeneous Assembly and Fabrication of Devices, issued May 27, 2003.
US # 6,652,808 Methods for the Electronic Assembly and Fabrication of Devices, issued Nov. 25, 2003.
US #6,706,473 Systems and Devices for the Photoelectrophoretic Transport and Hybridization of Oligonucleotides, issued March 16, 2004.
P. Swanson, R. Gelbart, E. Atlas, L. Yang, T. Grogan, W.F. Butler, D.E. Ackley, and E. Sheldon. A fully multiplexed CMOS biochip for DNA Analysis. Sens. Actu. B, 64:22–30, 2000.
P.N. Gilles, D.J. Wu DJ, C.B. Foster, P.J. Dillion, and S.J. Channock. Single nucleotide polymorphic discrimination by an electronic dot blot assay on semiconductor microchips’. Nat. Biotechnol., 17(4):365–370, 1999.
N. Narasimhan and D. O’Kane. Validation of SNP Genotyping for Human Serum Paraoxonase Gene. Clin. Chem., 34(7):589–592, 2001.
R. Sosnowski, M.J. Heller, E. Tu, A. Forster, and R. Radtkey. Active Microelectronic Array System for DNA Hybridization, Genotyping and Pharmacogenomic Applications. Psychiat. Genet., 12:181–192, 2002.
Y.R. Sohni, J.R. Cerhan, and D.J. O’Kane. Microarray and Microfluidic Methodology for Genotyping Cytokine Gene Polymorphisms. Hum. Immunol., 64:990–997, 2003.
E.S. Pollak, L. Feng, H. Ahadian, and P. Fortina. Microarray-based genetic analysis for studying susceptibility to arterial and venous thrombotic disorders. Ital. Heart J., 2:569–572, 2001.
W.A. Thistlethwaite, L.M. Moses, K.C. Hoffbuhr, J.M. Devaney, and E.P. Hoffman. Rapid genotyping of common MeCP2 mutations with an electronic DNA microchip using serial differential hybridization. J. Mol. Diag., 5(2):121–126, 2003.
V.R. Mas, R.A. Fisher, D.G. Maluf, D.S. Wilkinson, T.G. Carleton, and A. Ferreira-Gonzalez. Hepatic artery thrombosis after liver transplantation and genetic factors: prothrombin G20210A polymorphism. Transplanation, 76(1):247–249, 2003.
R. Santacroce, A. Ratti, F. Caroli, B. Foglieni, A. Ferraris, L. Cremonesi, M. Margaglione, M. Seri, R. Ravazzolo, G. Restagno, B. Dallapiccola, E. Rappaport, E.S. Pollak, S. Surrey, M. Ferrari, and P. Fortina. Analysis of clinically relevant single-nucleotide polymorphisms by use of microelectric array technology. Clin. Chem., 48(12):2124–2130, 2002.
A. åsberg, K. Thorstensen, K. Hveem, and K. Bjerve. Hereditary hemochromatosis: the clinical significance of the S64C mutation. Genet. Test., 6(1):59–62, 2002.
J.G. Evans and C. Lee-Tataseo. Determination of the factor V leiden single-nucleotide polymorphism in a commercial clinical laboratory by use of NanoChip microelectric array technology. Clin. Chem., 48(9):1406–1411, 2002.
J. Cheng, E.L. Sheldon, L. Wu, A. Uribe, L.O. Gerrue, J. Carrino, M.J. Heller, and J.P. O’Connell. Electric field controlled preparation and hybridization analysis ofDNA/RNAfrom E. coli on microfabricated bioelectronic chips. Nat. Biotech., (16):541–546, 1998.
J. Cheng, E.L. Sheldon, L. Wu, M.J. Heller, and J. O’Connell. Isolation of Cultured Cervical Carcinoma Cells Mixed with Peripheral Blood Cells on a Bioelectronic Chip. Anal. Chem., (70):2321–2326, 1998.
Y. Huang, J. Sunghae, M. Duhon, M.J. Heller, B. Wallace, and X. Xu. Dielectrophoretic separation and gene expression profiling on microelectronic chip arrays. Anal. Chem., 74:3362–3371, 2002.
T. Forster. Dicuss. Faraday Soc., 27:7, 1959.
R.A. Flynn, A.L. Birkbeck, M. Gross, M. Ozkan, M. Shao, M. Wang, and S.C. Esener. Parallel transport of biological cells using individually addressable VSCEL arrays as optical tweezers. Sens. Actuat. B, 6363:1–5, 2003.
A.L. Birkbeck, R.A. Flynn, M. Ozkan, D. Song, M. Gross, and S.C. Esener. VCSEL arrays as micromanipulators in chip-based biosystem. Biomed. Microdev., 5(1):61–67, 2003.
M. Ozkan, M. Wang, C. Ozkan, R.A. Flynn, and S.C. Esener. Optical manipulation of objects and biological cells in microfluidic devices. Biomed. Microdev., 5(1):47–54, 2003.
M. Ozkan, T. Pisanic, J. Sheel, C. Barrow, S. Esener, and S. Bhatia. “Electro-Optical Platform for the Manipulation of Live Cells”, Special issue on the Biomolecular Interface, Langmuir, 19(5):1532–1538, 2003.
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Heller, M.J., Ozkan, C.S., Ozkan, M. (2006). Use of Electric Field Array Devices for Assisted Assembly of DNA Nanocomponents and Other Nanofabrication Applications. In: Ferrari, M., Ozkan, M., Heller, M.J. (eds) BioMEMS and Biomedical Nanotechnology. Springer, Boston, MA. https://doi.org/10.1007/978-0-387-25843-0_6
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DOI: https://doi.org/10.1007/978-0-387-25843-0_6
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