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BioMEMS and Biomedical Nanotechnology pp 437–446Cite as

Fluorescent Lanthanide Labels with Time-Resolved Fluorometry in DNA Analysis

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Abstract

Some lanthanide (Eu3+, Tb3+, Sm3+, and Dy3+) complexes are known to be luminescent. Compared with organic fluorescent compounds, the fluorescence of lanthanide chelates has several special different properties; (1) The lifetime of the lanthanide chelates is very long; that of europium(III) and terbium(III) chelates usually ranges from several hundred microseconds to more than one millisecond, and that of samarium(III) and dysprosium (III), 10 to 100 microseconds. (2) Stokes shifts are very large: the chelates are excited by UV light (310–350 nm) and emit fluorescence in the visible region. (3) The emission profiles are sharp, having a full width at half maximum (FWHM) of only ~10 nm.

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References

  1. E. Soini and I. Hemmilä. Clin. Chem., 25:353–361, 1979.

    Google Scholar 

  2. I. Hemmilä. Clin. Chem., 31:359–370, 1985.

    Google Scholar 

  3. E. Soini and T. Lövgren. CRC Crit. Rev. Anal. Chem., 18:105–154, 1987.

    Google Scholar 

  4. E.P. Diamandis. Clin. Biochem., 21:139–150, 1988.

    Google Scholar 

  5. E.P. Diamandis and T.K. Christopoulos. Anal. Chem., 62:1149A–1157A, 1990.

    Google Scholar 

  6. I. Hemmilä. Appl. Fluoresc. Technol., 1:1–8, 1988.

    Google Scholar 

  7. I. Hemmilä. Scand. J. Clin. Lab. Invest., 48:389–400, 1988.

    Google Scholar 

  8. E.F.G. Dickson, A. Pollak, and E.P. Diamandis. Pharmac. Ther., 66:207–235, 1995.

    Article  Google Scholar 

  9. I. Hemmilä and S. Webb. DDT, 2:373–381, 1997.

    Google Scholar 

  10. J. Yuan and K. Matsumoto. Bunseki Kagaku, 48:1077–1083, 1999.

    Google Scholar 

  11. K. Matsumoto and J. Yuan. Metal Ions in Biological Systems. A. Sigel and H. Sigel (eds.), Marcel Dekker, New York, Basel, Chapter 6, Vol. 40, pp.191–232, 2003.

    Google Scholar 

  12. S.I. Weissman. J. Chem. Phys., 10:214–216, 1942.

    Article  Google Scholar 

  13. H.G. Huang, K. Hiraki, and Y. Nishikawa. Nippon Kagaku Kaishi, 66–70, 1981.

    Google Scholar 

  14. R. Reisfeld and C.K. Jörgensen. Lasers and Excited States of Rare Earths, Springer, Berlin, 1977.

    Google Scholar 

  15. Y.-Y. Xu and I. Hemmilä. Anal. Chim. Acta, 256:9–16, 1992.

    Article  Google Scholar 

  16. W.D. Horrocks and D.R. Sudnick. J. Am. Chem. Soc., 101:334–340, 1979.

    Article  Google Scholar 

  17. A.G. Goryushko and N.K. Davidenko. Zh. Neorg. Khim., 25:2666–2668, 1980.

    Google Scholar 

  18. J. Yuan and K. Matsumoto. Anal. Sci., 12:695–699, 1996.

    Google Scholar 

  19. J. Yuan and K. Matsumoto. J. Pharm. Biomed. Anal., 15:1397–1403, 1997.

    Article  Google Scholar 

  20. J. Yuan, K. Matsumoto, and H. Kimura. Anal. Chem., 70:596–601, 1998.

    Article  Google Scholar 

  21. R.A. Evangelista, A. Pollak, B. Allore, E.F. Templeton, R.C. Morton, and E.P. Diamandis. Clin. Biochem., 21:173–177, 1988.

    Article  Google Scholar 

  22. G. Mathis. Clin Chem., 39:1953–1959, 1993.

    Google Scholar 

  23. A.K. Saha, K. Kross, E.D. Kloszewski, D.A. Upson, J.L. Toner, R.A. Snow, C.D.V. Black, and V.C. Desai. J. Am. Chem. Soc., 115:11032–11033, 1993.

    Article  Google Scholar 

  24. D. Horiguchi, K. Sasamoto, H. Terasawa, H. Mochizuki, and Y. Ohkura. Chem. Pharm. Bull., 42:972–975, 1994.

    Google Scholar 

  25. S. Inoue and R. Honda. J. Clin. Microbiol., 28:1469–1472, 1990.

    Google Scholar 

  26. T. Sekiya, M. Fushimi, H. Hori, S. Hirohashi, S. Nishimura, and T. Sugimura. Proc. Natl. Acad. Sci. U.S.A., 81:4771–4775, 1984.

    Article  Google Scholar 

  27. I.C. Hsu, R.A. Metcalf, T. Sun, J.A. Welsh, N.J. Wang, and C.C. Harris. Nature, 350:427–429, 1991.

    Article  Google Scholar 

  28. M. Samiotaki, M. Kwiatkowski, N. Ylitalo, and U. Landegren. Anal. Biochem., 253:156–161, 1997.

    Article  Google Scholar 

  29. P. Heinonen, A. Iitiä, T. Torresani, and T. Lövgren. Clin. Chem., 43:1142–1150, 1997.

    Google Scholar 

  30. S. Bortolin, T.K. Christopoulos, and M. Verhaegen. Anal. Chem., 68:834–840, 1996.

    Article  Google Scholar 

  31. P.C. Ioannou and T.K. Christopoulos. Anal. Chem., 70:698–702, 1998.

    Article  Google Scholar 

  32. K. Yoshikawa, J. Yuan, K. Matsumoto, and H. Kimura. Anal. Sci., 15:121–124, 1999.

    Article  Google Scholar 

  33. S. Sueda, J. Yuan, and K. Matsumoto. Bioconjugate Chem., 11:827–831, 2000.

    Article  Google Scholar 

  34. G. Wang, J. Yuan, and K. Matsumoto. Anal. Biochem., 299:169–172, 2001.

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry and Advanced Research Institute for Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo, 169-8555, Japan

    Takuya Nishioka, Jingli Yuan & Kazuko Matsumoto

Authors
  1. Takuya Nishioka
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  2. Jingli Yuan
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  3. Kazuko Matsumoto
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Editor information

Editors and Affiliations

  1. Department of Biomedical Engineering, University of Texas Health Science Center, Houston, TX

    Mauro Ferrari Ph.D. (Editor-in-Chief, Professor, Brown Institute of Molecular Medicine Chairman, Professor of Experimental Therapeutics, Professor of Bioengineering, Professor of Biochemistry and Molecular Biology, President) (Editor-in-Chief, Professor, Brown Institute of Molecular Medicine Chairman, Professor of Experimental Therapeutics, Professor of Bioengineering, Professor of Biochemistry and Molecular Biology, President)

  2. Anderson Cancer Center, University of Texas M.D., Houston, TX

    Mauro Ferrari Ph.D. (Editor-in-Chief, Professor, Brown Institute of Molecular Medicine Chairman, Professor of Experimental Therapeutics, Professor of Bioengineering, Professor of Biochemistry and Molecular Biology, President) (Editor-in-Chief, Professor, Brown Institute of Molecular Medicine Chairman, Professor of Experimental Therapeutics, Professor of Bioengineering, Professor of Biochemistry and Molecular Biology, President)

  3. Rice University, Houston, TX

    Mauro Ferrari Ph.D. (Editor-in-Chief, Professor, Brown Institute of Molecular Medicine Chairman, Professor of Experimental Therapeutics, Professor of Bioengineering, Professor of Biochemistry and Molecular Biology, President) (Editor-in-Chief, Professor, Brown Institute of Molecular Medicine Chairman, Professor of Experimental Therapeutics, Professor of Bioengineering, Professor of Biochemistry and Molecular Biology, President)

  4. University of Texas Medical Branch, Galveston, TX

    Mauro Ferrari Ph.D. (Editor-in-Chief, Professor, Brown Institute of Molecular Medicine Chairman, Professor of Experimental Therapeutics, Professor of Bioengineering, Professor of Biochemistry and Molecular Biology, President) (Editor-in-Chief, Professor, Brown Institute of Molecular Medicine Chairman, Professor of Experimental Therapeutics, Professor of Bioengineering, Professor of Biochemistry and Molecular Biology, President)

  5. Texas Alliance for NanoHealth, Houston, TX

    Mauro Ferrari Ph.D. (Editor-in-Chief, Professor, Brown Institute of Molecular Medicine Chairman, Professor of Experimental Therapeutics, Professor of Bioengineering, Professor of Biochemistry and Molecular Biology, President) (Editor-in-Chief, Professor, Brown Institute of Molecular Medicine Chairman, Professor of Experimental Therapeutics, Professor of Bioengineering, Professor of Biochemistry and Molecular Biology, President)

  6. Dept. of Electrical Engineering, University of California Riverside, Riverside, California, USA

    Mihrimah Ozkan

  7. Dept. of Bioengineering, University of California, San Diego La Jolla, California, USA

    Michael J. Heller

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Nishioka, T., Yuan, J., Matsumoto, K. (2006). Fluorescent Lanthanide Labels with Time-Resolved Fluorometry in DNA Analysis. In: Ferrari, M., Ozkan, M., Heller, M.J. (eds) BioMEMS and Biomedical Nanotechnology. Springer, Boston, MA. https://doi.org/10.1007/978-0-387-25843-0_13

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  • DOI: https://doi.org/10.1007/978-0-387-25843-0_13

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-387-25564-4

  • Online ISBN: 978-0-387-25843-0

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