Abstract
Measurements of intracellular calcium concentrations ([Ca2+]i)using intracellularly trapped fluorescent dyes have become a popular way to determine changes in [Ca2+]i in individual cells. Techniques for performing such experiments are well documented (5,9,14,15). More recent advancements in confocal laser scanning microscopy (CLSM) have made it possible to perform these measurements even within tissue slices. Preparation of the CLSM tissue and performing an experiment on a tissue slice is slightly different than that for single cells. In this chapter, we provide an overview of the basics of confocal microscopy and subsequently describe how to set up and perform an experiment on brain the Ca2+ indicator indo-1 depending on the solution used during slice cutting and dye loading procedures.
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Scheenen, W., Carmignoto, G. (2002). Confocal Imaging of Calcium Signaling in Cells from Acute Brain Slices. In: Merighi, A., Carmignoto, G. (eds) Cellular and Molecular Methods in Neuroscience Research. Springer, New York, NY. https://doi.org/10.1007/978-0-387-22460-2_16
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DOI: https://doi.org/10.1007/978-0-387-22460-2_16
Publisher Name: Springer, New York, NY
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