Part of the
Biological and Medical Physics, Biomedical Engineering
book series (BIOMEDICAL)
Suppose you discovered a computerized factory turning out small cars, and you wanted to know how those cars were assembled and how they functioned. One way to identify essential components would be to remove those components one at a time and then characterize the resulting defects. For example, if you removed the drive shaft, the engine would run but the drive wheels would not turn, so the car would be paralyzed. If you knew the computer program, you could do this at will by removing the instructions for fabrication or assembly of drive shafts. If you did not know those instructions, or indeed even what a car might be, you could still learn a great deal by changing the program at random (e.g., by making mutants). This is how things proceeded in the early days of bacterial chemotaxis. One mutagenized cells, isolated mutants with interesting defects (e.g., cells with flagella that failed to spin), and then mapped the gene. Given the gene, one could identify the gene product. Now things are much easier. The genetic program is known in detail, and one can modify it in any way that one desires. For example, one can amplify a specific gene by using the polymerase chain reaction (PCR), change its sequence at will, and put it back into the chromosome by homologous recombination.
KeywordsMethylation Level Response Regulator Receptor Occupancy Histidine Kinase Maltose Binding Protein
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Abram, D., and H. Koffler. 1964. In vitro formation of flagella-like filaments and other structures from flagellin. J. Mol. Biol.
Adler, J. 1969. Chemoreceptors in bacteria. Science
Asakura, S., G. Eguchi, and T. Iino. 1964. Reconstitution of bacterial flagella in vitro. J. Mol. Biol
Astbury, W. T., E. Beighton, and C. Weibull. 1955. The structure of bacterial flagella. Symp. Soc. Exp. Biol.
Berg, H. C., and R. A. Anderson. 1973. Bacteria swim by rotating their flagellar filaments. Nature
Calladine, C. R. 1978. Change in waveform in bacterial flagella: the role of mechanics at the molecular level. J. Mol. Biol.
Falke, J. J., R. B. Bass, S. L. Butler, S. A. Chervitz, and M. A. Danielson. 1997. The two-component signaling pathway of bacterial chemotaxis: a molecular view of signal transduction by receptors, kinases, and adaptation enzymes. Anna. Rev. Cell Dev. Biol.
Falke, J. J., and G. L. Hazelbauer. 2001. Transmembrane signaling in bacterial chemoreceptors. Trends Biochem. Sci.
Iino, T., Y. Komeda, K. Kutsukake, et al. 1988. New unified nomenclature for the flagellar genes of Escherichia coli
and Salmonella typhimurium
. Microbiol Rev
. 52:533–535.Google Scholar
Kim, K. K., H. Yokota, and S.-H. Kim. 1999. Four helical bundle structure of the cytoplasmic domain of a serine chemotaxis receptor. Nature
Kim, S.-H., W. Wang, and K. K. Kim. 2002. Dynamic and clustering model of bacterial chemotaxis receptors: structural basis for signaling and high sensitivity. Proc. Natl. Acad. Sci. USA
Lee, S.-Y., H. S. Cho, J. G. Pelton, et al. 2001. Crystal structure of an activated response regulator bound to its target. Nature Struct. Biol.
Namba, K., and F. Vonderviszt. 1997. Molecular architecture of bacterial flagellum. Q. Rev. Biophys.
Parkinson, J. S., and E. C. Kofoid. 1992. Communication modules in bacterial signaling proteins. Anna. Rev. Genet.
Piekarski, G., and H. Ruska. 1939. Übermikroskopische Darstellung von Bakteriengeisseln. Klin. Wochenschr.
Reichert, K. 1909. Über die Sichtbarmachung der Geissein und die Geisseibewegung der Bakterien. Zentralbl. Bakteriol Parasitenk. Infektionskr. Abt. 1 Orig.
Samatey, F. A., K. Imada, S. Nagashima, et al. 2001. Structure of the bacterial flagellar protofilament and implications for a switch for supercoiling. Nature
Silverman, M., and M. Simon. 1974. Fiagellar rotation and the mechanism of bacterial motility. Nature
Taylor, B. L., I. B. Zhulin, and M. S. Johnson. 1999. Aerotaxis and other energy-sensing behavior in bacteria. Annu. Rev. Microbiol.
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