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Application of Neutron Reflectivity for Studies of Biomolecular Structures and Functions at Interfaces

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Neutron Applications in Earth, Energy and Environmental Sciences

Part of the book series: Neutron Scattering Applications and Techniques ((NEUSCATT))

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Abstract

Structures and functions of cell membranes are of central importance in understanding processes such as cell signaling, chemotaxis, redox transformation, biofilm formation, and mineralization occurring at interfaces. This chapter provides an overview of the application of neutron reflectivity (NR) as a unique tool for probing biomolecular structures and mechanisms as a first step toward understanding protein–protein, protein–lipid, and protein–mineral interactions at the membrane–substrate interfaces. Emphasis is given to the review of existing literature on the assembly of biomimetic membrane systems, such as supported membranes for NR studies, and demonstration of model calculations showing the potential of NR to elucidate molecular fundamentals of microbial cell–mineral interactions and structure–functional relationships of electron transport pathways. The increased neutron flux afforded by current and upcoming neutron sources holds promise for elucidating detailed processes such as phase separation, formation of microdomains, and membrane interactions with proteins and peptides in biological systems.

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Authors and Affiliations

  1. Environmental Sciences Division, Oak Ridge National Laboratory, MS-6038, 1 Bethel Valley Road, Oak Ridge, TN 37831-6038, USA

    Alexander Johs

  2. Environmental Sciences Division, Oak Ridge National Laboratory, MS-6038, Oak Ridge, TN 37831-6038, USA

    Liyuan Liang

  3. Environmental Sciences Division, Oak Ridge National Laboratory, MS-6036, Oak Ridge, TN 37831-6036, USA

    Baohua Gu & Wei Wang

  4. Neutron Scattering Science Division, Oak Ridge National Laboratory, MS 6475, 1 Bethel Valley Road, Oak Ridge, TN 37831-6475, USA

    John F. Ankner

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Correspondence to Alexander Johs .

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  1. Oak Ridge National Laboratory, Oak Ridge, TN, USA

    Liyuan Liang

  2. Universitádi Perugia, Italy

    Romano Rinaldi

  3. Institut Laue-Langevin, Grenoble, France

    Helmut Schober

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Johs, A., Liang, L., Gu, B., Ankner, J.F., Wang, W. (2009). Application of Neutron Reflectivity for Studies of Biomolecular Structures and Functions at Interfaces. In: Liang, L., Rinaldi, R., Schober, H. (eds) Neutron Applications in Earth, Energy and Environmental Sciences. Neutron Scattering Applications and Techniques. Springer, Boston, MA. https://doi.org/10.1007/978-0-387-09416-8_16

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