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Activity-Based Protein Profiling at the Host–Pathogen Interface

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Book cover Activity-Based Protein Profiling

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 420))

Abstract

Activity-based protein profiling (ABPP) is a technique for selectively detecting reactive amino acids in complex proteomes with the aid of chemical probes. Using probes that target catalytically active enzymes, ABPP can rapidly define the functional proteome of a biological system. In recent years, this approach has been increasingly applied to globally profile enzymes active at the host–pathogen interface of microbial infections. From in vitro co-culture systems to animal models of infection, these studies have revealed enzyme-mediated mechanisms of microbial pathogenicity, host immunity, and metabolic adaptation that dynamically shape pathogen interactions with the host.

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Acknowledgements

We gratefully acknowledge Pamela Chang, Joshua Gendron, Yannick Jacob, and Lindsay Triplett for providing helpful feedback on this manuscript. Y. K. was supported by an NIH Predoctoral Training Grant (T32GM067543).

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Authors and Affiliations

  1. Microbial Sciences Institute, Yale University, West Haven, CT, 06516, USA

    Yekaterina Kovalyova & Stavroula K. Hatzios

  2. Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT, 06511, USA

    Stavroula K. Hatzios

  3. Department of Chemistry, Yale University, New Haven, CT, 06511, USA

    Yekaterina Kovalyova & Stavroula K. Hatzios

Authors
  1. Yekaterina Kovalyova
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  2. Stavroula K. Hatzios
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Correspondence to Stavroula K. Hatzios .

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Editors and Affiliations

  1. Department of Chemistry, The Scripps Research Institute, La Jolla, CA, USA

    Benjamin F. Cravatt

  2. Department of Chemistry, University of Virginia, Charlottesville, VA, USA

    Ku-Lung Hsu

  3. Department of Chemistry, Boston College, Chestnut Hill, MA, USA

    Eranthie Weerapana

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Kovalyova, Y., Hatzios, S.K. (2018). Activity-Based Protein Profiling at the Host–Pathogen Interface. In: Cravatt, B., Hsu, KL., Weerapana, E. (eds) Activity-Based Protein Profiling. Current Topics in Microbiology and Immunology, vol 420. Springer, Cham. https://doi.org/10.1007/82_2018_129

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