Abstract
FCRLA is homologous to receptors for the Fc portion of IgG (FcγR) and is located in the same region of human chromosome one, but has several unusual and unique features. It is a soluble resident ER protein retained in this organelle by unknown mechanisms involving the N-terminal domain, a disordered domain with three Cys residues in close proximity in the human protein. Unlike the FcγRs, FCRLA is not glycosylated and has no transmembrane region. FCRLA is included in this CTMI volume on IgM-binding proteins because it binds IgM in the ER, but quite surprisingly, given the isotype-restricted ligand specificity of the other FcRs, it also binds all other Ig isotypes so far tested, IgG and IgA. In the case of IgM, there is even preferential binding of the secretory and not the transmembrane form. Among B cells, FCRLA is most highly expressed in the germinal center and shows little expression in plasma cells. Based on these observations, we propose that one human FCRLA function is to stop GC B cells from secreting IgM, which would act as a decoy receptor, thus preventing the B cells from capturing antigen, processing it, and presenting the antigen-derived peptides to T follicular helper cells. Without help from these T cells, there would be limited B cell isotype switching, proliferation, and differentiation. On the other hand, FCRLA is downregulated in plasma cells, where IgM secretion is an essential function. FCRLA may also act as a chaperone involved by unknown mechanisms in the proper assembly of Ig molecules of all isotypes.
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References
Aleyd E, Heineke MH, van Egmond M (2015) The era of the immunoglobulin a Fc receptor FcαRI; its function and potential as target in disease. Immunol Rev 268:123–138
Ben Mkaddem S, Rossato E, Heming N, Monteiro RC (2013) Anti-inflammatory role of the IgA Fc receptor (CD89): from autoimmunity to therapeutic perspectives. Autoimmun Rev 12:666–669
Bentham J, Morris DL, Cunninghame Graham DS, Pinder CL, Tombleson P, Behrens TW, Martin J, Fairfax BP, Knight JC, Chen L, Replogle J, Syvanen AC, Ronnblom L, Graham RR, Wither JE, Rioux JD, Alarcon-Riquelme ME, Vyse TJ (2015) Genetic association analyses implicate aberrant regulation of innate and adaptive immunity genes in the pathogenesis of systemic lupus erythematosus. Nat Genet 47:1457–1464
Berken A, Benacerraf B (1966) Properties of antibodies cytophilic for macrophages. J Exp Med 123:119–144
Bork P, Holm L, Sander C (1994) The immunoglobulin fold. Structural classification, sequence patterns and common core. J Mol Biol 242:309–320
Brooks K, Yuan D, Uhr JW, Krammer PH, Vitetta ES (1983) Lymphokine-induced IgM secretion by clones of neoplastic B cells. Nature 302:825–826
Bruno ME, Frantz AL, Rogier EW, Johansen FE, Kaetzel CS (2011) Regulation of the polymeric immunoglobulin receptor by the classical and alternative NF-kappaB pathways in intestinal epithelial cells. Mucosal Immunol 4:468–478
Chikaev NA, Bykova EA, Najakshin AM, Mechetina LV, Volkova OY, Peklo MM, Shevelev AY, Vlasik TN, Roesch A, Vogt T, Taranin AV (2005) Cloning and characterization of the human FCRL2 gene. Genomics 85:264–272
Corcoran LM, Tarlinton DM (2016) Regulation of germinal center responses, memory B cells and plasma cell formation-an update. Curr Opin Immunol 39:59–67
Davila S, Froeling FE, Tan A, Bonnard C, Boland GJ, Snippe H, Hibberd ML, Seielstad M (2010) New genetic associations detected in a host response study to hepatitis B vaccine. Genes Immun 11:232–238
Davis RS, Li H, Chen CC, Wang YH, Cooper MD, Burrows PD (2002) Definition of an Fc receptor-related gene (FcRX) expressed in human and mouse B cells. Int Immunol 14:1075–1083
De Silva NS, Klein U (2015) Dynamics of B cells in germinal centres. Nat Rev Immunol 15:137–148
DeFranco AL 2016 The germinal center antibody response in health and disease. F1000Res 5:
Facchetti F, Cella M, Festa S, Fremont DH, Colonna M (2002) An unusual Fc receptor-related protein expressed in human centroblasts. Proc Natl Acad Sci USA 99:3776–3781
Foss S, Watkinson R, Sandlie I, James LC, Andersen JT (2015) TRIM21: a cytosolic Fc receptor with broad antibody isotype specificity. Immunol Rev 268:328–339
Franco A, Damdinsuren B, Ise T, Dement-Brown J, Li H, Nagata S, Tolnay M (2013) Human Fc receptor-like 5 binds intact IgG via mechanisms distinct from those of Fc receptors. Journal of immunology 190:5739–5746
Gething MJ (1999) Role and regulation of the ER chaperone BiP. Semin Cell Dev Biol 10:465–472
Ghumra A, Shi J, McIntosh RS, Rasmussen IB, Braathen R, Johansen FE, Sandlie I, Mongini PK, Areschoug T, Lindahl G, Lewis MJ, Woof JM, Pleass RJ (2009) Structural requirements for the interaction of human IgM and IgA with the human Fcα/μ receptor. Eur J Immunol 39:1147–1156
Haas IG (1991) BiP—a heat shock protein involved in immunoglobulin chain assembly. Curr Top Microbiol Immunol 167:71–82
Halaby DM, Mornon JP (1998) The immunoglobulin superfamily: an insight on its tissular, species, and functional diversity. J Mol Evol 46:389–400
Hanson QM, Barb AW (2015) A perspective on the structure and receptor binding properties of immunoglobulin G Fc. Biochemistry 54:2931–2942
Hebert DN, Molinari M (2007) In and out of the ER: protein folding, quality control, degradation, and related human diseases. Physiol Rev 87:1377–1408
Inozume T, Matsuzaki Y, Kurihara S, Fujita T, Yamamoto A, Aburatani H, Shimada S, Kawakami Y (2005) Novel melanoma antigen, FCRL/FREB, identified by cDNA profile comparison using DNA chip are immunogenic in multiple melanoma patients. Int J Cancer 114:283–290
Inozume T, Mitsui H, Okamoto T, Matsuzaki Y, Kawakami Y, Shibagaki N, Shimada S (2007) Dendritic cells transduced with autoantigen FCRLA induce cytotoxic lymphocytes and vaccinate against murine B-cell lymphoma. J Invest Dermatol 127:2818–2822
Kanamaru Y, Blank U, Monteiro RC (2007) IgA Fc receptor I is a molecular switch that determines IgA activating or inhibitory functions. Contrib Nephrol 157:148–152
Kim YE, Hipp MS, Bracher A, Hayer-Hartl M, Hartl FU (2013) Molecular chaperone functions in protein folding and proteostasis. Annu Rev Biochem 82:323–355
Kim K, Bang SY, Joo YB, Kim T, Lee HS, Kang C, Bae SC (2016) Response to intravenous cyclophosphamide treatment for lupus nephritis associated with polymorphisms in the FCGR2B-FCRLA locus. J Rheumatol 43:1045–1049
King LB, Corley RB (1989) Characterization of a presecretory phase in B-cell differentiation. Proc Natl Acad Sci USA 86:2814–2818
Kubagawa H, Oka S, Kubagawa Y, Torii I, Takayama E, Kang DW, Gartland GL, Bertoli LF, Mori H, Takatsu H, Kitamura T, Ohno H, Wang JY (2009) Identity of the elusive IgM Fc receptor (FcmuR) in humans. J Exp Med 206:2779–2793
Kubagawa H, Kubagawa Y, Jones D, Nasti TH, Walter MR, Honjo K (2014) The old but new IgM Fc receptor (FcμR). Curr Top Microbiol Immunol 382:3–28
Lee YK, Brewer JW, Hellman R, Hendershot LM (1999) BiP and immunoglobulin light chain cooperate to control the folding of heavy chain and ensure the fidelity of immunoglobulin assembly. Mol Biol Cell 10:2209–2219
Li FJ, Won WJ, Becker EJ Jr, Easlick JL, Tabengwa EM, Li R, Shakhmatov M, Honjo K, Burrows PD, Davis RS (2014) Emerging roles for the FCRL family members in lymphocyte biology and disease. Curr Top Microbiol Immunol 382:29–50
Mallery DL, McEwan WA, Bidgood SR, Towers GJ, Johnson CM, James LC (2010) Antibodies mediate intracellular immunity through tripartite motif-containing 21 (TRIM21). Proc Natl Acad Sci USA 107:19985–19990
Maltais LJ, Lovering RC, Taranin AV, Colonna M, Ravetch JV, Dalla-Favera R, Burrows PD, Cooper MD, Davis RS (2006) New nomenclature for Fc receptor-like molecules. Nat Immunol 7:431–432
Masir N, Jones M, Pozzobon M, Marafioti T, Volkova OY, Mechetina LV, Hansmann ML, Natkunam Y, Taranin AV, Mason DY (2004) Expression pattern of FCRL (FREB, FcRX) in normal and neoplastic human B cells. Br J Haematol 127:335–343
Masuda K, Davis RS, Maruyama T, Zhang J, He T, Cooper MD, Wang J, Burrows PD (2005) FcRY, an Fc receptor related gene differentially expressed during B lymphocyte development and activation. Gene 363:32–40
Masuda K, Mori H, Ohara O, Nakayama M, Wang JY, Burrows PD (2010) Defining the immunological phenotype of Fc receptor-like B (FCRLB) deficient mice: confounding role of the inhibitory FcγRIIb. Cell Immunol 266:24–31
McEwan WA, Falcon B, Vaysburd M, Clift D, Oblak AL, Ghetti B, Goedert M, James LC (2017) Cytosolic Fc receptor TRIM21 inhibits seeded tau aggregation. Proc Natl Acad Sci USA 114:574–579
Mechetina LV, Najakshin AM, Volkova OY, Guselnikov SV, Faizulin RZ, Alabyev BY, Chikaev NA, Vinogradova MS, Taranin AV (2002) FCRL, a novel member of the leukocyte Fc receptor family possesses unique structural features. Eur J Immunol 32:87–96
Munro S, Pelham HR (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907
Ni M, Lee AS (2007) ER chaperones in mammalian development and human diseases. FEBS Lett 581:3641–3651
Nischan J, Gatalica Z, Curtis M, Lenk GM, Tromp G, Kuivaniemi H (2009) Binding sites for ETS family of transcription factors dominate the promoter regions of differentially expressed genes in abdominal aortic aneurysms. Circ Cardiovasc Genet 2:565–572
Pincetic A, Bournazos S, DiLillo DJ, Maamary J, Wang TT, Dahan R, Fiebiger BM, Ravetch JV (2014) Type I and type II Fc receptors regulate innate and adaptive immunity. Nat Immunol 15:707–716
Pyzik M, Rath T, Lencer WI, Baker K, Blumberg RS (2015) FcRn: the architect behind the immune and nonimmune functions of IgG and albumin. J immunol 194:4595–4603
Rath T, Kuo TT, Baker K, Qiao SW, Kobayashi K, Yoshida M, Roopenian D, Fiebiger E, Lencer WI, Blumberg RS (2013) The immunologic functions of the neonatal Fc receptor for IgG. J Clin Immunol 33(Suppl 1):S9–17
Ravetch JV, Bolland S (2001) IgG Fc receptors. Annu Rev Immunol 19:275–290
Roopenian DC, Sun VZ (2010) Clinical ramifications of the MHC family Fc receptor FcRn. J Clin Immunol 30:790–797
Santiago T, Kulemzin SV, Reshetnikova ES, Chikaev NA, Volkova OY, Mechetina LV, Zhao M, Davis RS, Taranin AV, Najakshin AM, Hendershot LM, Burrows PD (2011) FCRLA is a resident endoplasmic reticulum protein that associates with intracellular Igs, IgM, IgG and IgA. Int Immunol 23:43–53
Schreeder DM, Cannon JP, Wu J, Li R, Shakhmatov MA, Davis RS (2010) Cutting edge: FcR-like 6 is an MHC class II receptor. J Immunol 185:23–27
Shibuya A, Honda S (2015) Immune regulation by Fcα/μ receptor (CD351) on marginal zone B cells and follicular dendritic cells. Immunol Rev 268:288–295
Shibuya A, Sakamoto N, Shimizu Y, Shibuya K, Osawa M, Hiroyama T, Eyre HJ, Sutherland GR, Endo Y, Fujita T, Miyabayashi T, Sakano S, Tsuji T, Nakayama E, Phillips JH, Lanier LL, Nakauchi H (2000) Fcα/μ receptor mediates endocytosis of IgM-coated microbes. Nat Immunol 1:441–446
Spillane KM, Tolar P (2017) B cell antigen extraction is regulated by physical properties of antigen-presenting cells. J cell biol 216:217–230
Tesar DB, Bjorkman PJ (2010) An intracellular traffic jam: Fc receptor-mediated transport of immunoglobulin G. Curr Opin Struct Biol 20:226–233
Turner H, Kinet JP (1999) Signalling through the high-affinity IgE receptor Fc epsilonRI. Nature 402:B24–B30
Wang H, Coligan JE, Morse HC 3rd (2016) Emerging Functions of Natural IgM and its Fc Receptor FCMR in Immune Homeostasis. Front Immunol 7:99
Wilson TJ, Colonna M (2005) A new Fc receptor homolog, FREB2, found in germinal center B cells. Genes Immun 6:341–346
Wilson TJ, Gilfillan S, Colonna M (2010) Fc receptor-like a associates with intracellular IgG and IgM but is dispensable for antigen-specific immune responses. J. Immunol. 185:2960–2967
Wilson TJ, Fuchs A, Colonna M (2012) Cutting edge: human FcRL4 and FcRL5 are receptors for IgA and IgG. J Immunol 188:4741–4745
Wohner M, Tagoh H, Bilic I, Jaritz M, Poliakova DK, Fischer M, Busslinger M (2016) Molecular functions of the transcription factors E2A and E2-2 in controlling germinal center B cell and plasma cell development. J Exp Med 213:1201–1221
Yang X, Zhao Q, Zhu L, Zhang W (2013) The three complementarity-determining region-like loops in the second extracellular domain of human Fc alpha/mu receptor contribute to its binding of IgA and IgM. Immunobiology 218:798–809
Zhang Y, Garcia-Ibanez L, Toellner KM (2016) Regulation of germinal center B-cell differentiation. Immunol Rev 270:8–19
Zhou XJ, Cheng FJ, Qi YY, Zhao YF, Hou P, Zhu L, Lv JC, Zhang H (2013) FCGR2B and FCRLB gene polymorphisms associated with IgA nephropathy. PLoS ONE 8:e61208
Acknowledgements
We thank our many colleagues for insightful discussions, including Tim Wilson, Linda Hendershot, Alexander Taranin, Randall Davis, Hiromi Kubagawa, Ludmilla Mechetina, John Kearney, and Max Cooper. We thank NIH for funding.
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Blackburn, T.E., Santiago, T., Burrows, P.D. (2017). FCRLA—A Resident Endoplasmic Reticulum Protein that Associates with Multiple Immunoglobulin Isotypes in B Lineage Cells. In: Kubagawa, H., Burrows, P. (eds) IgM and Its Receptors and Binding Proteins. Current Topics in Microbiology and Immunology, vol 408. Springer, Cham. https://doi.org/10.1007/82_2017_40
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