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Genetics and Genomics of Brachypodium pp 129–145Cite as

The microRNAs of Brachypodium

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Part of the book series: Plant Genetics and Genomics: Crops and Models ((PGG,volume 18))

Abstract

Since the proposal of Brachypodium distachyon as a model organism, a substantial amount of research has been done investigating the miRNAs which aid in the post-transcriptional gene regulation of this grass. Techniques such as the sequencing of small RNA libraries have allowed for the identification and annotation of over 500 miRNAs present in the Brachypodium genome. Furthermore, experimental evidence for the cleavage of a large number of target mRNAs has also been found, including a few instances of differential target cleavage by distinct miRNA family members. This research has not only provided the community with a wealth of publicly available sequencing data, but it has also led to many insights regarding how these small 21–24 nt RNA molecules may play critical roles in different tissues, stress responses, and developmental processes of Brachypodium.

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Abbreviations

AGO1:

Agonaute

ESTs:

Expressed Sequence Tags

GEO:

Gene Expression Omnibus

GSSs:

Genome Survey Sequences

PARE:

Parallel Analysis of RNA Ends

RISC:

RNA-induced silencing complex

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Acknowledgements

We thank Dr. Dong-Hoon Jeong, Hallym University, for helpful comments on the manuscript. Brachypodium research in our lab was supported by the Office of Biological and Environmental Research of the Department of Energy under Plant Feedstock Genomics for Bioenergy award number DE-FG02-07ER64450 to P.J.G. K.R.F. was supported, in part, by NIH/NIGMS CBI Training Grant 5T32GM008550-21.

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Authors and Affiliations

  1. Delaware Biotechnology Institute, University of Delaware, Newark, DE, USA

    Karl R. Franke & Pamela J. Green

  2. Department of Biological Sciences, University of Delaware, Newark, DE, USA

    Karl R. Franke

  3. Department of Plant and Soil Sciences, University of Delaware, Newark, DE, USA

    Pamela J. Green

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  1. Karl R. Franke
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Correspondence to Pamela J. Green .

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Editors and Affiliations

  1. DOE Joint Genome Institute, Walnut Creek, California, USA

    John P. Vogel

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Franke, K.R., Green, P.J. (2015). The microRNAs of Brachypodium . In: Vogel, J. (eds) Genetics and Genomics of Brachypodium. Plant Genetics and Genomics: Crops and Models, vol 18. Springer, Cham. https://doi.org/10.1007/7397_2015_9

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