Abstract
Measurements of trace metals as parts of homeostatic networks for essential and nonessential metals in microbial cells need sensitive high-resolution techniques. The term “metallome” denotes metals and metalloid species within cells, encompassing both the inorganic element content and their complexes with biomolecules, especially with peptides and proteins. Elucidation of the physiological roles of metals and their bioinorganic speciation requires a set of microanalytical purification, separation, and identification methods. This chapter summarizes analytical tools useful to investigate bacterial responses to metal stress.
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Wesenberg, D., Bleuel, C., Krauss, GJ. (2007). A Glossary of Microanalytical Tools to Assess the Metallome. In: Nies, D.H., Silver, S. (eds) Molecular Microbiology of Heavy Metals. Microbiology Monographs, vol 6. Springer, Berlin, Heidelberg. https://doi.org/10.1007/7171_2006_077
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