Skip to main content

Protein Body Biogenesis in Cereal Endosperms

  • Chapter
  • First Online:
Book cover Endosperm

Part of the book series: Plant Cell Monographs ((CELLMONO,volume 8))

Abstract

Developing cereal endosperms accumulate storage proteins as a reserve to support the early postgerminating phase of plant growth and development. These storage proteins are concentrated and packaged into a specialized organelle, the protein body, which are derived from the endoplasmic reticulum lumen or vacuole. In this chapter, we describe the biochemical and cellular events that underlies the basis for the use of these two intracellular sites for storage protein deposition and accumulation.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bagga S, Adams H, Kemp JD, Sengupta-Gopalan C (1995) Accumulation of 15-kD zein in novel protein bodies in transgenic tobacco. Plant Physiol 107:13–23

    PubMed  CAS  Google Scholar 

  • Bagga S, Adams HP, Rodriguez FD, Kemp JD, Sengupta-Gopalan C (1997) Coexpression of the maize δ-zein and β-zein genes results in stable accumulation of δ-zein in endoplasmic reticulum-derived protein bodies formed by β-zein. Plant Cell 9:1683–1696

    Article  PubMed  CAS  Google Scholar 

  • Bechtel DR, Juliano BO (1980) Formation of protein bodies in the starchy endosperm of rice (Oryza sativa L.): a reinvestigation. Ann Bot 65:684–691

    Google Scholar 

  • Bechtel DB, Gaines RL, Pomeranz Y (1982) Protein secretion in wheat endosperm—formation of the matrix protein. Cereal Chem 59:336–343

    Google Scholar 

  • Buttrose MS (1963) Ultrastructure of the developing wheat endosperm. Aust J Biol Sci 16:305–317

    Google Scholar 

  • Cameron-Mills V, von Wettstein D (1980) Protein body formation in the developing barley endosperm. Carlsberg Res Commun 45:577–594

    Article  CAS  Google Scholar 

  • Campbell WP, Lee JW, O'Brien TP, Smart MG (1981) Endosperm morphology and protein body formation in developing wheat grains. Aust J Plant Physiol 8:5–19

    Article  CAS  Google Scholar 

  • Choi S-B, Wang C, Muench DG, Ozawa K, Franceschi VR, Wu Y, Okita T (2000) Messenger RNA targeting of rice seed storage proteins to specific ER subdomains. Nature 407:765–767

    Article  PubMed  CAS  Google Scholar 

  • Coleman CE, Larkins BA (1999) The prolamins of maize. In: Shewry PR, Casey R (eds) Seed proteins. Kluwer, Dordrecht, pp 109–139

    Google Scholar 

  • Coleman CE, Herman EM, Takasaki K, Larkins BA (1996) The maize γ-zein sequesters α-zein and stabilizes its accumulation in protein bodies of transgenic tobacco endosperm. Plant Cell 8:2335–2345

    Article  PubMed  CAS  Google Scholar 

  • Coleman CE, Yoho PR, Escobar S, Ogawa M (2004) The accumulation of α-zein in transgenic tobacco endosperm is stabilized by co-expression of β-zein. Plant Cell Physiol 45:864–871

    Article  PubMed  CAS  Google Scholar 

  • Esen A, Stetler DA (1992) Immunocytochemical localization of δ-zein in the protein bodies of maize endosperm cells. Am J Bot 79:243–248

    CAS  Google Scholar 

  • Galili G (1997) The prolamin storage proteins of wheat and its relatives. In: Larkins BA, Vasil IK (eds) Cellular and molecular biology of plant seed development. Kluwer, Dordrecht, pp 221–256

    Google Scholar 

  • Galili G, Herman EM (1997) Protein bodies: storage vacuoles in seeds. Adv Bot Res 25:113–140

    Article  CAS  Google Scholar 

  • Galili G, Altschuler Y, Levanony H, Giorini-Silfen S, Shimoni Y, Shani N, Karchi H (1995) Assembly and transport of wheat storage proteins. J Plant Physiol 145:626–631

    CAS  Google Scholar 

  • Geli MI, Torrent M, Ludevid D (1994) Two structural domains mediate two sequential events in γ-zein targeting: protein endoplasmic reticulum retention and protein body formation. Plant Cell 6:1911–1922

    Article  PubMed  CAS  Google Scholar 

  • Hamada S, Ishiyama K, Choi SB, Wang C, Singh S, Kawai N, Franceschi VR, Okita TW (2003a) The transport of prolamine RNAs to prolamine protein bodies in living rice endosperm cells. Plant Cell 15:2253–64

    Article  PubMed  CAS  Google Scholar 

  • Hamada S, Ishiyama K, Sakulsingharoj C, Choi SB, Wu Y, Wang C, Singh S, Kawai N, Messing J, Okita TW (2003b) Dual regulated RNA transport pathways to the cortical region in developing rice endosperm. Plant Cell 15:2265–72

    Article  PubMed  CAS  Google Scholar 

  • Hinchliffe DJ, Kemp JD (2002) β-Zein protein bodies sequester and protect the 18-kD δ-zein protein from degradation. Plant Sci 163:741–752

    Article  CAS  Google Scholar 

  • Hoffman LM, Donaldson DD, Bookland R, Rashka K, Herman EM (1987) Synthesis and protein deposition of maize 15-kD zein in transgenic tobacco seeds. EMBO J 6:3213–3221

    PubMed  CAS  Google Scholar 

  • Kawagoe Y, Suzuki K, Tasaki M, Yasuda H, Akagi K, Katoh E, Nishizawa NK, Ogawa M, Takaiwa F (2005) The critical role of disulfide bond formation in protein sorting in the endosperm of rice. Plant Cell 17:1141–1153

    Article  PubMed  CAS  Google Scholar 

  • Kim CS, Woo Y-M, Clore AM, Burnett RJ, Carneiro NP, Larkins BA (2002) Zein protein interactions, rather than the asymmetric distribution of zein mRNAs on endoplasmic reticulum membranes, influence protein body formation in maize endosperm. Plant Cell 14:655–672

    Article  PubMed  CAS  Google Scholar 

  • Kim WT, Franceschi VR, Krishnan HB, Okita TW (1988) Formation of wheat protein bodies: involvement of the Golgi apparatus in gliadin transport. Planta 173:173–182

    Article  Google Scholar 

  • Kim WT, Li X, Okita TW (1993) Expression of storage protein multigene families in developing rice endosperm. Plant Cell Physiol 34:595–603

    CAS  Google Scholar 

  • Krishnan HB, Pueppke SG (1993) Nucleotide sequence of an abundant rice seed globulin: homology with the high molecular weight glutelins of wheat, rye and triticale. Biochem Biophys Res Commun 193:460–466

    Article  PubMed  CAS  Google Scholar 

  • Krishnan HB, Franceschi VR, Okita TW (1986) Immunochemical studies on the role of the Golgi complex in protein body formation in rice seeds. Planta 169:471–480

    Article  CAS  Google Scholar 

  • Krishnan HB, White JA, Pueppke SG (1990) Immunocytochemcial evidence for the involvement of the Golgi apparatus in the transport of the vacuolar protein, gamma-secalin, in rye (Secale cereale) endosperm. Cereal Chem 67:360–366

    CAS  Google Scholar 

  • Krishnan HB, White JA, Pueppke SG (1991) Immunocytochemical localization of wheat prolamins in the lumen of the rough endoplasmic reticulum. Can J Bot 69:2574–2577

    Article  Google Scholar 

  • Krishnan HB, White JA, Pueppke SG (1992) Characterization and localization of rice (Oryza sativa L.) seed globulins. Plant Sci 81:1–11

    Article  CAS  Google Scholar 

  • Kumamaru T, Satoh H, Iwata N, Omura T, Ogawa M (1987) Mutants for rice storage proteins. 3. Genetic analysis of mutants for storage proteins of protein bodies in the starchy endosperm. Theor Jpn J Genet 62:333–339

    Article  Google Scholar 

  • Kumamaru T, Satoh H, Iwata N, Omura T, Ogawa O, Tanaka K (1988) Mutants for rice storage proteins. 1. Screening of mutants for rice storage proteins of protein bodies in the starchy endosperm. Theor Appl Genet 76:11–16

    Article  CAS  Google Scholar 

  • Larkins BA, Hurkman WJ (1978) Synthesis and deposition of zein protein bodies of maize endosperm. Plant Physiol 62:256–263

    Article  PubMed  CAS  Google Scholar 

  • Larkins BA, Vasil IK (1997) Cellular and molecular biology of plant seed development. Kluwer, Dordrecht

    Google Scholar 

  • Lending CR, Larkins BA (1989) Changes in the zein composition of protein bodies during maize endosperm development. Plant Cell 1:1011–1023

    Article  PubMed  CAS  Google Scholar 

  • Lending CR, Kriz AL, Larkins BA, Bracker CE (1988) Structure of maize protein bodies and immunocytochemical localization of zeins. Protoplasma 143:51–62

    Article  CAS  Google Scholar 

  • Levanony H, Rubin R, Altschuler Y, Galili G (1992) Evidence for a novel route of wheat storage proteins to vacuoles. J Cell Biol 119:1117–1128

    Article  PubMed  CAS  Google Scholar 

  • Li X, Wu Y, Zhang D-Z, Gillikin JW, Boston R, Franceschi VR, Okita TW (1993a) Rice prolamine protein body biosynthesis: a BiP-mediated process. Science 262:1054–1056

    Article  PubMed  CAS  Google Scholar 

  • Li X, Franceschi VR, Okita TW (1993b) Segregation of storage protein mRNAs on the rough endoplasmic reticulum membranes of rice endosperm cells. Cell 72:869–879

    Article  PubMed  CAS  Google Scholar 

  • Miflin BJ, Burgess SR, Shewry PR (1981) The development of protein bodies in the storage tissues of seeds: subcellular separation of homogenates of barley, maize and wheat endosperm and pea cotyledon. J Exp Bot 32:199–219

    Article  Google Scholar 

  • Møgelsvang S, Simpson DJ (1998) Changes in the levels of seven proteins involved in polypeptide folding and transport during endosperm development of two barley genotypes differing in storage protein localisation. Plant Mol Biol 36:541–552

    Article  PubMed  Google Scholar 

  • Muench DG, Wu Y, Zang Y, Li X, Boston R, Okita TW (1997) Molecular cloning, expression and subcellular localization of a BiP homolog from rice endosperm tissue. Plant Cell Physiol 38:404–412

    PubMed  CAS  Google Scholar 

  • Muench DG, Ogawa M, Okita TW (1999) The prolamins of rice. In: Shewry PR, Casey R (eds) Seed proteins. Kluwer, Dordrecht, pp 93–108

    Google Scholar 

  • Ogawa M, Kumamaru T, Satoh H, Iwata N, Omura T, Kasai Z, Tanaka K (1987) Purification of protein body-I of rice seed and its polypeptide composition. Plant Cell Physiol 28:1517–1527

    CAS  Google Scholar 

  • Ogawa M, Kumamaru T, Satoh H, Omura T, Park T, Shintaku K, Baba K (1989) Mutants for rice storage proteins. 2. Isolation and characterization of protein bodies from rice mutants. Theor Appl Genet 78:305–310

    Article  CAS  Google Scholar 

  • Oparka N, Harris N (1982) Rice protein body formation: all types are initiated by dilation of the endoplasmic reticulum. Planta 154:184–188

    Article  Google Scholar 

  • Parker ML (1982) Protein accumulation in developing endosperm of a high-protein line of Triticum dicoccoides. Plant Cell Environ 5:237–243

    Google Scholar 

  • Rechinger KB, Simpson DJ, Svendsen I, Cameron-Mills V (1993) A role for γ 3-hordein in the transport and targeting of prolamin polypeptides to the vacuole of developing barley endosperm. Plant J 4:841–853

    Article  PubMed  CAS  Google Scholar 

  • Rost TL (1971) Fine structure of endosperm protein bodies in Setaria lutescens (Gramineae). Protoplasma 73:475–479

    Article  Google Scholar 

  • Rubin R, Levanony H, Galili G (1992) Evidence for the presence of two different types of protein bodies in wheat endosperm. Plant Physiol 99:718–724

    Article  PubMed  CAS  Google Scholar 

  • Shewry PR, Casey R (eds) (1999) Seed proteins. Kluwer, Dordrecht

    Google Scholar 

  • Shewry PR, Napier NA, Tatham AS (1995) Seed storage proteins: structures and biosynthesis. Plant Cell 7:945–956

    Article  PubMed  CAS  Google Scholar 

  • Shotwell M, Larkins BA (1989) The biochemistry and molecular biology of seed storage proteins. In: Marcus E (ed) The biochemistry of plants: a comprehensive treatise. Academic, Orlando, FL, pp 296–345

    Google Scholar 

  • Shull JM, Watterson JJ, Kirleis AW (1992) Purification and immunocytochemical localization of karifins in Sorghum bicolor (L Moench) endosperm. Protoplasma 171:64–74

    Article  CAS  Google Scholar 

  • Takaiwa F, Ogawa M, Okita TW (1999) Rice glutelin. In: Casey R, Shewry PR (eds) Seed proteins. Chapman and Hall, London, pp 401–426

    Google Scholar 

  • Takemoto Y, Coughlan SJ, Okita TW, Satoh H, Ogawa M, Kumamaru T (2002) Protein disulfide isomerase is essential for the localization of prolamin and glutelin storage proteins to separate intracellular compartments. Plant Physiol 128:1212–1222

    Article  PubMed  CAS  Google Scholar 

  • Tanaka K, Sugimoto T, Ogawa M, Kasai Z (1980) Isolation and characterization of two types of protein bodies in the rice endosperm. Agric Biol Chem 44:1633–1639

    CAS  Google Scholar 

  • Tooze J, Kern HF, Fuller SD, Howell KE (1989) Condensation-sorting events in the rough endoplasmic reticulum of exocrine pancreatic cells. J Cell Biol 109:35–50

    Article  PubMed  CAS  Google Scholar 

  • Washida H, Sugino A, Messing J, Esen A, Okita TW (2004) Asymmetric localization of seed storage protein RNAs to distinct subdomains of the endoplasmic reticulum in developing maize endosperm cells. Plant Cell Physiol 45:1830–1837

    Article  PubMed  CAS  Google Scholar 

  • Woo Y-M, Hu DW-N, Larkins BA, Jung R (2001) Genomics analysis of genes expressed in maize endosperm identifies novel seed proteins and clarifies patterns of zein gene expression. Plant Cell 13:2297–2317

    Article  PubMed  CAS  Google Scholar 

  • Yamagata H, Tanaka K (1986) The site of synthesis and accumulation of rice storage proteins. Plant Cell Physiol 27:135–145

    CAS  Google Scholar 

  • Yamagata H, Tanaka K, Kasai Z (1982) Evidence for a precursor form of rice glutelin subunits. Agric Biol Chem 46:321–322

    CAS  Google Scholar 

  • Yamagata H, Tamura K, Tanaka K, Kasai Z (1986) Cell-free synthesis of rice prolamin. Plant Cell Physiol 27:1419–1422

    CAS  Google Scholar 

  • Yamagata T, Kato H, Kuroda S, Abe S, Davies E (2003) Uncleaved legumin in developing maize endosperm: identification, accumulation and putative subcellular localization. J Exp Bot 54:913–922

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to T. W. Okita .

Editor information

Odd-Arne Olsen

Rights and permissions

Reprints and permissions

Copyright information

© 2007 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Kumamaru, T., Ogawa, M., Satoh, H., Okita, T.W. (2007). Protein Body Biogenesis in Cereal Endosperms. In: Olsen, OA. (eds) Endosperm. Plant Cell Monographs, vol 8. Springer, Berlin, Heidelberg. https://doi.org/10.1007/7089_2007_115

Download citation

Publish with us

Policies and ethics