Skip to main content
SpringerLink
Log in

Storage Proteins and Peroxidase Activity During Zygotic and Somatic Embryogenesis of Firs (Abies sp.)

  • Chapter
  • First Online:
Somatic Embryogenesis

Part of the book series: Plant Cell Monographs ((CELLMONO,volume 2))

Abstract

Somatic embryogenesis was initiated from immature embryos of Abies concolor (Gord. et Glend), A. numidica De Lann. and A. cilicica Carr., A. alba Mill. as well as in hybrid fir A. cilicica × A. nordmanniana. Soluble protein profiles and peroxidase activity were compared in developing zygotic and somatic embryos of silver fir (A. alba Mill.). On the basis of sodium dodecyl sulfate polyacrylamide gel electrophoresis of soluble proteins a high degree of homology was established between the two types of embryos. A higher peroxidase activity was registered throughout zygotic embryogenesis than during somatic embryo development but the opposite was true at the stage of mature embryos. Isoperoxidase composition reflected more efficiently the developmental stages of zygotic embryogenesis than those of somatic embryogenesis.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Boulay MP, Gupta PK, Krogstrup P, Durzan DJ (1988) Development of somatic embryos from cell suspension cultures of Norway spruce (Picea abies karst). Plant Cell Rep 7:134–137

    Article  Google Scholar 

  2. Cyrr DR, Webster FB, Roberts DR (1991) Biochemical events during germination and early growth of somatic embryos and seed of interior spruce (Picea glauca engelmanii complex). Seed Sci Res 1:91–97

    Google Scholar 

  3. Dong JZ, Dunstan DI (2000) Molecular biology of somatic embryogenesis in conifers. In: Jain SM, Minocha SC (eds) Molecular biology of woody plants. vol 1. Kluwer, Dordrecht, p 51–87

    Google Scholar 

  4. Flinn BS, Roberts DR, Taylor JEP (1991) Evaluation of somatic embryos of interior spruce. Characterization and developmental regulation of storage proteins. Physiol Plant 82:624–632

    Article  CAS  Google Scholar 

  5. Flinn BS, Roberts DR, Newton CH, Webster FB, Taylor JEP (1993) Storage protein gene expression in zygotic and somatic embryos of interior spruce. Physiol Plant 89:719–730

    Article  CAS  Google Scholar 

  6. Gajdošová A, Vooková B, Kormut'ák A, Libiaková G, Doležel J (1995) Induction, protein composition and DNA ploidy level of embryogenic calli of silver fir and its hybrids. Biol Plant 37:169–176

    Google Scholar 

  7. Gaspar T, Kevers C, Penel C, Crèvecoeur M, Greppin H (1988) Biochemical characterization of normal and habituated sugarbeet calli. Relationship with anatomy, habituation and organogenesis. Potsdamer Forsch 57:21–30

    Google Scholar 

  8. Gifford DJ (1988) An electrophoretic analysis of the seed protein from Pinus monticola and eight other species of pine. Can J Bot 66:1808–1812

    CAS  Google Scholar 

  9. Gifford DJ, Tolley MC (1989) The seed proteins of white spruce and their mobilization following germination. Physiol Plant 77:254–261

    CAS  Google Scholar 

  10. Gresshoff PM, Doy CH (1972) Development and differentiation of haploid Lycopersicum esculentum (tomato). Planta 107:161–170

    Google Scholar 

  11. Guevin TG, Kirby EG (1997) Induction of embryogenesis in cultured mature zygotic embryos of Abies fraseri (Pursh) Poir. Plant Cell Tissue Org Cult 49:219–222

    CAS  Google Scholar 

  12. Guevin TG, Micah V, Kirby EG (1994) Somatic embryogenesis in cultured mature zygotic embryos of Abies balsamea. Plant Cell Tissue Org Cult 37:205–208

    CAS  Google Scholar 

  13. Hakman I (1993) Embryology in Norway spruce (Picea abies). An analysis of the composition of seed storage proteins and deposition of storage reserves during seed development and somatic embryogenesis. Physiol Plant 87:148–159

    Article  CAS  Google Scholar 

  14. Hakman I, Stabel P, Engström P, Eriksson T (1990) Storage protein accumulation during zygotic and somatic embryo development in Picea abies (Norway spruce). Physiol Plant 80:441–445

    Article  CAS  Google Scholar 

  15. Hřib J, Vooková B, Kormut'ák A (1997) Biochemical differences between normal callus and embryogenic suspensor mass of silver fir. Biol Plant 39:507–513

    Google Scholar 

  16. Hristoforoglu K, Schmidt J, Bolhar-Nordenkamp H (1995) Development and germination of Abies alba somatic embryos. Plant Cell Tissue Org Cult 40:277–284

    Google Scholar 

  17. Hrubcová M, Cvikrová M, Eder J (1994) Peroxidase activities and content of phenolic acids in embryogenic and non-embryogenic alfalfa cell suspension cultures. Biol Plant 39:175–182

    Google Scholar 

  18. Jensen U, Lixue Ch (1991) Abies seed protein profile divergent from other Pinaceae. Taxon 40:435–440

    Google Scholar 

  19. Kormut'ák A, Salaj T, Matúšová R, Vooková B (2003) Biochemistry of zygotic and somatic embryogenesis in silver fir (Abies alba Mill.). Acta Biol Cracoviensia, Ser Botan 45:59–62

    Google Scholar 

  20. Klimaszewska K, Morency F, Jones-Overton C, Cooke J (2004) Accumulation pattern and identification of seed storage proteins in zygotic embryos of Pinus strobus and in somatic embryos from different maturation treatments. Physiol Plant 121:682–690

    Article  CAS  Google Scholar 

  21. Krajňáková J, Häggman H (1997) Regeneration of Abies cephalonica through somatic embryogenesis. In: Identification and control of phase changes in rejuvenation. COST 822, 4th Meeting of WG3, Nitra, Slovakia, 15–19 October 1997, pp 102–104

    Google Scholar 

  22. Misra S, Green MJ (1990) Developmental gene expression in conifer embryogenesis and germination. I. Seed proteins and protein body composition of mature embryo and the megagametophyte of white spruce (Picea glauca [Moench] Voss.). Plant Sci 68:163–173

    CAS  Google Scholar 

  23. Misra S, Attree SM, Leal J, Fowke LC (1993) Effect of abscisic acid, osmoticum, and desiccation on synthesis of storage proteins during the development of white spruce somatic embryos. Ann Bot 71:11–22

    Article  CAS  Google Scholar 

  24. Mo LH, Egertsdotter U, von Arnold S (1996) Secretion of specific extracellular proteins by somatic embryos of Picea abies is depended on embryo morphology. Ann Bot 77:143–152

    Article  CAS  Google Scholar 

  25. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    CAS  Google Scholar 

  26. Nørgaard JV (1997) Somatic embryo maturation and plant regeneration in Abies nordmanniana LK. Plant Sci 124:211–221

    Article  Google Scholar 

  27. Nørgaard JV, Krogstrup P (1991) Cytokinin induced somatic embryogenesis from immature embryos of Abies nordmanniana LK. Plant Cell Rep 9:509–513

    Google Scholar 

  28. Nørgaard JV, Krogstrup P (1995) Somatic embryogenesis in Abies spp. In: Jain SM, Gupta PK, Newton RJ (eds) Somatic embryogenesis in woody plants, vol 3. Gymnosperms. Kluwer, Dordrecht, pp 341–355

    Google Scholar 

  29. Rajbhandari N, Stomp A-E (1997) Embryogenic callus induction in Fraser fir. Hort Sci 32:737–738

    Google Scholar 

  30. Roth R, Schmidt J (1998) Induction of embryogenic suspensor masses from cotyledons of zygotic seedlings of Abies alba. In: Symp. Zum Gedenken an die 100 Wiederkehr der Begründung der Gewebekultur durch Gottlieb Haberlandt, Vienna, 8–9 October 1998, p 45

    Google Scholar 

  31. Salajová T, Salaj J (2001) Somatic embryogenesis and plantlet regeneration from cotyledon explants isolated from emblings and seedlings of hybrid firs. J Plant Physiol 158:747–755

    Google Scholar 

  32. Salajová T, Salaj J (2003=2004) Somatic embryo formation on mature Abies alba × Abies cephalonica zygotic embryo explants. Biol Plant 47:7–11

    Google Scholar 

  33. Salajová T, Salaj J, Jásik J, Kormut'ák A, Hakman I (1996) Embryogenic culture initiation and somatic embryo development in hybrid firs (Abies alba × Abies cephalonica and Abies alba × Abies numidica). Plant Cell Rep 15:527–530

    Google Scholar 

  34. Salaj T, Matúšová R, Salaj J (2004) The effect of carbohydrates and polyethylene glycol on somatic embryo maturation in hybrid fir Abies alba × A. numidica. Acta Biol Cracov Bot 46:159–167

    Google Scholar 

  35. Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J For Res 50:199–204

    CAS  Google Scholar 

  36. Schuller A, Reuther G, Geier T (1989) Somatic embryogenesis from seed explants of Abies alba. Plant Cell Tissue Org Cult 17:53–58

    Google Scholar 

  37. Schuller A, Kirchner-Ness R, Reuther G (2000) Interaction of plant growth regulators and organic C and N components in the formation and maturation of Abies alba somatic embryos. Plant Cell Tiss Org Cult 60:23–31

    CAS  Google Scholar 

  38. Stabel P, Eriksson T, Engström P (1990) Changes in protein synthesis upon cytokinin-mediated adventitious bud induction and during seedling development in Norway spruce, Picea abies. Plant Physiol 92:1174–1183

    CAS  Google Scholar 

  39. Thorpe TA (2000) Somatic embryogenesis: morphogenesis, physiology, biochemistry and molecular biology. Korean J Plant Tissue Cult 27:245–258

    Google Scholar 

  40. Vooková B, Kormut'ák A (2001) Effect of sucrose concentration, charcoal, and indole-3-butyric acid on germination of Abies numidica somatic embryos. Biol Plant 44:181–184

    Article  Google Scholar 

  41. Vooková B, Kormut'ák A (2002a) Effect of plant growth regulators treatment on proliferation and somatic embryo development of Algerian fir (Abies numidica De Lann.). Folia Oecolog 29:141–146

    Google Scholar 

  42. Vooková B, Kormut'ák A (2002b) Some futures of somatic embryo maturation of Algerian fir. In Vitro Cell Dev Biol 38:549–561

    Google Scholar 

  43. Vooková B, Kormut'ák A (2003) Plantlet regeneration in Abies cilicica and Abies cilicica × Abies nordmanniana hybrid via somatic embryogenesis. Turk J Bot 27:71–76

    Google Scholar 

  44. Vooková B, Kormut'ák A (2004) Propagation of some Abies species by somatic embryogenesis. Acta Univ Latv Biol 676:257–260

    Google Scholar 

  45. Vooková B, Kormut'ák A, Hřib J (2001) Effect of myo-inositol on somatic embryogenesis of Abies numidica. J Appl Bot 75:46–49

    Google Scholar 

  46. Vooková B, Matúšová R, Kormut'ák A (2003) Secondary somatic embryogenesis in Abies numidica De Lann. Biol Plant 46:513–517

    Article  Google Scholar 

  47. Zimmerman JL (1993) Somatic embryogenesis: A model for early development of higher plants. Plant Cell 5:1411–1423

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

Financial support of the work from the Slovak Grant Agency VEGA, project no. 2/3192/24 is highly appreciated.

Author information

Authors and Affiliations

  1. Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademicka 2, P.O. Box 39A, 950 07, Nitra, Slovakia

    A. Kormut'ák & B. Vooková

Authors
  1. A. Kormut'ák
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. Vooková
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. Kormut'ák .

Editor information

A. Mujib Jozef Šamaj

Rights and permissions

Reprints and permissions

About this chapter

Cite this chapter

Kormut'ák, A., Vooková, B. Storage Proteins and Peroxidase Activity During Zygotic and Somatic Embryogenesis of Firs (Abies sp.). In: Mujib, A., Šamaj, J. (eds) Somatic Embryogenesis. Plant Cell Monographs, vol 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/7089_021

Download citation

Publish with us

Policies and ethics

Search

Navigation