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Somatic Embryogenesis pp 343–354Cite as

In Vitro Culture of Arabidopsis Embryos

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Part of the book series: Plant Cell Monographs ((CELLMONO,volume 2))

Abstract

Arabidopsis thaliana is currently the most important model organism for basic molecular plant research. It is also a favourable model for developmental biology, as its embryogenesis follows a nearly invariant pattern of cell divisions and cell type specifications. Study of embryogenesis can involve genetic, physiological or biochemical approaches, but is always limited by the inaccessibility of the embryos which develop deep inside maternal tissue. Thus, for developmental studies, there is an increasing demand for methods which allow embryogenesis under artificial conditions, providing better accessibility to experimental manipulation. In this chapter, we address theoretical aspects of embryo culture, give some thoughts on which embryo culture system is suited best for which application and finally discuss three current methods which have been successfully used in Arabidopsis embryo culture.

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Authors and Affiliations

  1. Zentrum für Molekularbiologie der Pflanzen, Universität Tübingen, Auf der Morgenstelle 3, 72076, Tübingen, Germany

    Michael Sauer & Jiří Friml

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  1. Michael Sauer
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  2. Jiří Friml
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Correspondence to Jiří Friml .

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A. Mujib Jozef Šamaj

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Sauer, M., Friml, J. In Vitro Culture of Arabidopsis Embryos. In: Mujib, A., Šamaj, J. (eds) Somatic Embryogenesis. Plant Cell Monographs, vol 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/7089_020

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