Abstract
The fission yeast Schizosaccharomyces pombe is well-suited for both genetic and biochemical analysis of meiotic recombination. Recent studies have revealed ∼50 gene products and two DNA intermediates central to recombination, which we place into a pathway from parental to recombinant DNA. We divide recombination into three stages – chromosome alignment accompanying nuclear “horsetail” movement, formation of DNA breaks, and repair of those breaks – and we discuss the roles of the identified gene products and DNA intermediates in these stages. Although some aspects of recombination are similar to those in the distantly related budding yeast Saccharomyces cerevisiae, other aspects are distinctly different. In particular, many proteins required for recombination in one species have no clear ortholog in the other, and the roles of identified orthologs in regulating recombination often differ. Furthermore, in S. pombe the dominant joint DNA molecule intermediates contain single Holliday junctions, and intersister joint molecules are more frequent than interhomolog types, whereas in S. cerevisiae interhomolog double Holliday junctions predominate. We speculate that meiotic recombination in other organisms shares features of each of these yeasts.
Keywords
- Synaptonemal Complex
- Meiotic Recombination
- Schizosaccharomyces Pombe
- Sister Chromatid Cohesion
- Holliday Junction
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References
Allers T, Lichten M (2001) Differential timing and control of noncrossover and crossover recombination during meiosis. Cell 106:47–57
Bähler J, Wyler T, Loidl J, Kohli J (1993) Unusual nuclear structures in meiotic prophase of fission yeast: a cytological analysis. J Cell Biol 121:241–256
Bishop DK, Park D, Xu L, Kleckner N (1992) DMC1: A meiosis-specific homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression. Cell 69:439–456
Boddy MN, Gaillard P-HL, McDonald WH, Shanahan P, Yates JR, Russell P (2001) Mus81-Eme1 are essential components of a Holliday junction resolvase. Cell 107:537–548
Borde V, Goldman ASH, Lichten M (2000) Direct coupling between meiotic DNA replication and recombination initiation. Science 290:806–809
Cao L, Alani E, Kleckner N (1990) A pathway for generation and processing of double-strand breaks during meiotic recombination in S. cerevisiae. Cell 61:1089–1101
Catlett MG, Forsburg SL (2003) Schizosaccharomyces pombe Rdh54 (TID1) acts with Rhp54 (RAD54) to repair meiotic double-strand breaks. Mol Biol Cell 14:4707–4720
Cervantes MD, Farah JA, Smith GR (2000) Meiotic DNA breaks associated with recombination in S. pombe. Mol Cell 5:883–888
Chikashige Y, Hiraoka Y (2001) Telomere binding of the Rap1 protein is required for meiosis in fission yeast. Curr Biol 11:1618–1623
Chikashige Y, Ding DQ, Funabiki H, Haraguchi T, Mashiko S, Yanagida M, Hiraoka Y (1994) Telomere-led premeiotic chromosome movement in fission yeast. Science 264:270–273
Chikashige Y, Tsutsumi C, Yamane M, Okamasa K, Haraguchi T, Hiraoka Y (2006) Meiotic proteins Bqt1 and Bqt2 tether telomeres to form the bouquet arrangement of chromosomes. Cell 125:59–69
Cooper JP, Watanabe Y, Nurse P (1998) Fission yeast Taz1 protein is required for meiotic telomere clustering and recombination. Nature 392:828–831
Cromie GA, Leach DR (2000) Control of crossing over. Mol Cell 6:815–826
Cromie GA, Hyppa RW, Taylor AF, Zakharyevich K, Hunter N, Smith GR (2006) Single Holliday junctions are intermediates of meiotic recombination. Cell 127:1167–1178
Cromie GA, Rubio CA, Hyppa RW, Smith GR (2005) A natural meiotic DNA break site in Schizosaccharomyces pombe is a hotspot of gene conversion, highly associated with crossing over. Genetics 169:595–605
Davis L, Smith GR (2005) Dynein promotes achiasmate segregation in Schizosaccharomyces pombe. Genetics 170:581–590
Davis L, Smith GR (2006) The meiotic bouquet promotes homolog interactions and restricts ectopic recombination in Schizosaccharomyces pombe. Genetics 174:167–177
de los Santos T, Hunter N, Lee C, Larkin B, Loidl J, Hollingsworth NM (2003) The Mus81/Mms4 endonuclease acts independently of double-Holliday junction resolution to promote a distinct subset of crossovers during meiosis in budding yeast. Genetics 164:81–94
DeVeaux LC, Smith GR (1994) Region-specific activators of meiotic recombination in Schizosaccharomyces pombe. Genes Dev 8:203–210
DeVeaux LC, Hoagland NA, Smith GR (1992) Seventeen complementation groups of mutations decreasing meiotic recombination in Schizosaccharomyces pombe. Genetics 130:251–262
Ding DQ, Sakurai N, Katou Y, Itoh T, Shirahige K, Haraguchi T, Hiraoka Y (2006) Meiotic cohesins modulate chromosome compaction during meiotic prophase in fission yeast. J Cell Biol 174:499–508
Doe CL, Ahn JS, Dixon J, Whitby MC (2002) Mus81-Eme1 and Rqh1 involvement in processing stalled and collapsed replication forks. J Biol Chem 277:32753–32759
Doe CL, Osman F, Dixon J, Whitby MC (2005) DNA repair by a Rad22-Mus81-dependent pathway that is independent of Rhp51. Nucleic Acids Res 32:5570–5581
Egel R (1984) Two tightly linked silent cassettes in the mating-type region of Schizosaccharomyces pombe. Curr Genet 8:199–203
Egel R, Willer M, Nielsen O (1989) Unblocking of meiotic crossing-over between the silent mating-type cassettes of fission yeast, conditioned by the recessive, pleiotropic mutant rik1. Curr Genet 15:407–410
Ellermeier C, Smith GR (2005) Cohesins are required for meiotic DNA breakage and recombination in Schizosaccharomyces pombe. Proc Natl Acad Sci USA 102:10952–10957
Ellermeier C, Schmidt H, Smith GR (2004) Swi5 acts in meiotic DNA joint molecule formation in Schizosaccharomyces pombe. Genetics 168:1891–1898
Fleck O, Lehmann E, Schär P, Kohli J (1999) Involvement of nucleotide-excision repair in msh2 pms1-independent mismatch repair. Nat Genet 21:314–317
Fox ME, Yamada T, Ohta K, Smith GR (2000) A family of CRE-related DNA sequences with meiotic recombination hotspot activity in Schizosaccharomyces pombe. Genetics 156:59–68
Gaillard P-HL, Noguchi E, Shanahan P, Russell P (2003) The endogenous Mus81-Eme1 complex resolves Holliday junctions by a nick and counternick mechanism. Mol Cell 12:747–759
Gregan J, Rabitsch PK, Sakem B, Csutak O, Latypov V, Lehmann E, Kohli J, Nasmyth K (2005) Novel genes required for meiotic chromosome segregation are identified by a high-throughput knockout screen in fission yeast. Curr Biol 15:1663–1669
Grewal SI, Klar AJ (1997) A recombinationally repressed region between mat2 and mat3 loci shares homology to centromeric repeats and regulates directionality of mating-type switching in fission yeast. Genetics 146:1221–1238
Grimm C, Bahler J, Kohli J (1994) M26 recombinational hotspot and physical conversion tract analysis in the ade6 gene of Schizosaccharomyces pombe. Genetics 135:41–51
Grishchuk AL, Kohli J (2003) Five RecA-like proteins of Schizosaccharomyces pombe are involved in meiotic recombination. Genetics 165:1031–1043
Gutz H (1971) Site specific induction of gene conversion in Schizosaccharomyces pombe. Genetics 69:317–337
Hagan I, Yanagida M (1995) The product of the spindle formation gene sad1 + associates with the fission yeast spindle pole body and is essential for viability. J Cell Biol 129:1033–1047
Haruta N, Kurokawa Y, Murayama Y, Akamatsu Y, Unzai S, Tsutsui Y, Iwasaki H (2006) The Swi5-Sfr1 complex stimulates Rhp51/Rad51 and Dmc1-mediated DNA strand exchange in vitro. Nat Struct Mol Biol 13:823–830
Hayase A, Takagi M, Miyazaki T, Oshiumi H, Shinohara M, Shinohara A (2004) A protein complex containing Mei5 and Sae3 promotes the assembly of the meiosis-specific RecA homolog Dmc1. Cell 119:927–940
Heyer WD, Li X, Rolfsmeier M, Zhang XP (2006) Rad54: the Swiss Army knife of homologous recombination? Nucleic Acids Res 34:4115–4125
Kanoh J, Ishikawa F (2001) spRap1 and spRif1, recruited to telomeres by Taz1, are essential for telomere function in fission yeast. Curr Biol 11:1624–1630
Keeney S (2001) Mechanism and control of meiotic recombination initiation. Curr Topics Dev Biol 52:1–53
Khasanov FK, Salakhova AF, Chepurnaja OV, Korolev VG, Bashkirov VI (2004) Identification and characterization of the rlp1 +, the novel Rad51 paralog in the fission yeast Schizosaccharomyces pombe. DNA Repair (Amst) 3:1363–1374
Kitajima TS, Kawashima SA, Watanabe Y (2004) The conserved kinetochore protein shugoshin protects centromeric cohesion during meiosis. Nature 427:510–517
Klar AJS, Bonaduce MJ (1991) swi6, a gene required for mating-type switching, prohibits meiotic recombination in the mat2-mat3 cold spot of fission yeast. Genetics 129:1033–1042
Klein F, Mahr P, Galova M, Buonomo SBC, Michaelis C, Nairz K, Nasmyth K (1999) A central role for cohesins in sister chromatid cohesion, formation of axial elements, and recombination during yeast meiosis. Cell 98:91–103
Kon N, Krawchuk MD, Warren BG, Smith GR, Wahls WP (1997) Transcription factor Mts1/Mts2 (Atf1/Pcr1, Gad7/Pcr1) activates the M26 meiotic recombination hotspot in Schizosaccharomyces pombe. Proc Natl Acad Sci USA 94:13756–13770
Kunz C, Fleck O (2001) Role of the DNA repair nucleases Rad13, Rad2 and Uve1 of Schizosaccharomyces pombe in mismatch correction. J Mol Biol 313:241–253
Loidl J (2006) S. pombe linear elements: the modest cousins of synaptonemal complexes. Chromosoma 115:260–271
Lorenz A, Wells JL, Pryce DW, Novatchkova FE, Eisenhaber F, McFarlane RJ, Loidl J (2004) S. pombe meiotic linear elements contain proteins related to synaptonemal complex components. J. Cell Sci 117:3343–3351
Lorenz A, Estreicher A, Kohli J, Loidl J (2006) Meiotic recombination proteins localize to linear elements in Schizosaccharomyces pombe. Chromosoma 115:330–340
Martín-Castellanos C, Blanco M, Rozalén AE, Pérez-Hidalgo L, García AI, Conde F, Mata J, Ellermeier C, Davis L, San-Segundo P, Smith GR, Moreno S (2005) A large-scale screen in S. pombe identifies seven novel genes required for critical meiotic events. Curr Biol 22:2056–2062
McPherson JP, Lemmers B, Chahwan R, Pamidi A, Migon E, Matysiak-Zablocki E, Moynahan ME, Essers J, Hanada K, Poonepalli A, Sanchez-Sweatman O, Khokha R, Kanaar R, Jasin M, Hande MP, Hakem R (2004) Involvement of mammalian Mus81 in genome integrity and tumor suppression. Science 304:1822–1826
Mieczkowski PA, Dominska M, Buck MJ, Gerton JL, Lieb JD, Petes TD (2006) Global analysis of the relationship between the binding of the Bas1p transcription factor and meiosis-specific double-strand DNA breaks in Saccharomyces cerevisiae. Mol Cell Biol 26:1014–1027
Miki F, Okazaki K, Shimanuki M, Yamamoto A, Hiraoka Y, Niwa O (2002) The 14-kDa dynein light chain-family protein Dlc1 is required for regular oscillatory nuclear movement and efficient recombination during meiotic prophase in fission yeast. Mol Biol Cell 3:930–946
Mizuno K-I, Emura Y, Baur M, Kohli J, Ohta K, Shibata T (1997) Remodeling of chromatin structure around a single nucleotide mutation in ade6-M26 that creates a meiotic recombination hotspot in fission yeast. Genes Dev 11:876–886
Mizuno K-I, Hasemi T, Ubukata T, Yamada T, Lehmann E, Kohli J, Watanabe Y, Iino Y, Yamamoto M, Fox ME, Smith GR, Murofushi H, Shibata T, Ohta K (2001) Counteracting regulation of chromatin remodeling at a fission yeast cAMP responsive element-related recombination hotspot by stress-activated protein kinase, cAMP-dependent kinase and meiosis regulators. Genetics 159:1467–1478
Molnar M, Bahler J, Sipiczki M, Kohli J (1995) The rec8 gene of Schizosaccaromyces pombe is involved in linear element formation, chromosome pairing and sister-chromatid cohesion during meiosis. Genetics 141:61–73
Molnar M, Parisi S, Kakihara Y, Nojima H, Yamamoto A, Hiraoka Y, Bozsik A, Sipiczki M, Kohli J (2001) Characterization of rec7, an early meiotic recombination gene in Schizosaccharomyces pombe. Genetics 157:519–532
Molnar M, Doll E, Yamamoto A, Hiraoka Y, Kohli J (2003) Linear element formation and their role in meiotic sister chromatid cohesion and chromosome pairing. J Cell Sci 114:1719–1731
Munz P (1994) An analysis of interference in the fission yeast Schizosaccharomyces pombe. Genetics 137:701–707
Munz P, Wolf K, Kohli J, Leupold U (1989) Genetics overview. In: Nasim A, Young P, Johnson BF (eds) Molecular Biology of the Fission Yeast. Academic Press, San Diego, pp 1–30
Nabeshima K, Kakihara Y, Hiraoka Y, Nojima H (2001) A novel meiosis-specific protein of fission yeast, Meu13p, promotes homologous pairing independently of homologous recombination. EMBO J 20:3871–3881
Nakaseko Y, Adachi Y, Funahashi S, Niwa O, Yanagida M (1986) Chromosome walking shows a highly homologous repetitive sequence present in all the centromere regions of fission yeast. EMBO J 5:1011–1021
Neale MJ, Pan J, Keeney S (2005) Endonucleolytic processing of covalent protein-linked DNA double-strand breaks. Nature 436:1053–1057
Niccoli T, Yamashita A, Nurse P, Yamamoto M (2004) The p150-Glued Ssm4p regulates microtubular dynamics and nuclear movement in fission yeast. J Cell Sci 117:5543–5556
Nichols MD, DeAngelis K, Keck JL, Berger JM (1999) Structure and function of an archaeal topoisomerase VI subunit with homology to the meiotic recombination factor Spo11. EMBO J 18:6177–6188
Niu H, Wan L, Baumgartner B, Schaefer D, Loidl J, Hollingsworth NM (2005) Partner choice during meiosis is regulated by Hop1-prompted dimerization of Mek1. Mol Biol Cell 16:5804–5818
Niwa O, Shimanuki M, Miki F (2000) Telomere-led bouquet formation facilitates homologous chromosome pairing and restricts ectopic interaction in fission yeast meiosis. EMBO J 19:3831–3840
Ogino K, Hirota K, Matsumoto S, Takeda T, Ohta K, Arai K, Masai H (2006) Hsk1 kinase is required for induction of meiotic dsDNA breaks without involving checkpoint kinases in fission yeast. Proc Natl Acad Sci USA 23:8131–8136
Ogino K, Masai H (2006) Rad3-Cds1 mediates coupling of initiation of meiotic recombination with DNA replication: Mei4-dependent transcription as a potential target of meiotic checkpoint. J Biol Chem 281:1338–1344
Olson LW, Eden U, Egel-Mitani M, Egel R (1978) Asynaptic meiosis in fission yeast? Heriditas 89:189–199
Osman F, Dixon J, Doe CL, Whitby MC (2003) Generating crossovers by resolution of nicked Holliday junctions: A role for Mus81-Eme1 in meiosis. Mol Cell 12:761–774
Parisi S, McKay MJ, Molnar M, Thompson MA, van der Speck PJ, van Drunen-Schoenmaker E, Kanaar R, Lehmann E, Hoeijmakers JHJ, Kohli J (1999) Rec8p, a meiotic recombination and sister chromatid cohesion phosphoprotein of the Rad21p family conserved from fission yeast to humans. Mol Cell Biol 19:3515–3528
Pasierbek P, Jantsch M, Melcher M, Schleiffer A, Schweizer D, Loidl J (2001) A Caenorhabditis elegans cohesion protein with functions in meiotic chromosome pairing and disjunction. Genes Dev 15:1349–1360
Paull TT, Gellert M (1998) The 3′ to 5′ exonuclease activity of Mre11 facilitates repair of DNA double-strand breaks. Mol Cell 1:969–979
Petes TD, Malone RE, Symington LS (1991) Recombination in yeast. In: Broach J, Jones E, Pringle J (eds) The Molecular and Cellular Biology of the Yeast Saccharomyces. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp 407–521
Ponticelli AS, Smith GR (1989) Meiotic recombination-deficient mutants of Schizosaccharomyces pombe. Genetics 123:45–54
Ponticelli AS, Smith GR (1992) Chromosomal context dependence of a eukaryotic recombinational hot spot. Proc Natl Acad Sci USA 89:227–231
Ponticelli AS, Sena EP, Smith GR (1988) Genetic and physical analysis of the M26 recombination hotspot of Schizosaccharomyces pombe. Genetics 119:491–497
Puizina J, Siroky J, Mokros P, Schweizer D, Riha K (2004) Mre11 deficiency in Arabidopsis is associated with chromosomal instability in somatic cells and Spo11-dependent genome fragmentation during meiosis. Plant Cell 16:1968–1978
Resnick MA (1976) The repair of double-strand breaks in DNA: a model involving recombination. J Theo Biol 59:97–106
Rijkers T, Van Den Ouweland J, Morolli B, Rolink AG, Baarends WM, Van Sloun PP, Lohman PH, Pastink A (1998) Targeted inactivation of mouse RAD52 reduces homologous recombination but not resistance to ionizing radiation. Mol Cell Biol 18:6423–6429
Saito TT, Tougan T, Kasama T, Okuzaki D, Nojima H (2004) Mcp7, a meiosis-specific coiled-coil protein of fission yeast, associates with Meu13 and is required for meiotic recombination. Nucleic Acids Res 32:3325–3339
Saito TT, Tougan T, Okuzaki D, Kasama T, Nojima H (2005) Mcp6, a meiosis-specific coiled-coil protein of Schizosaccharomyces pombe, localizes to the spindle pole body and is required for horsetail movement and recombination. J Cell Sci 118:447–459
Saito TT, Okuzaki D, Nojima H (2006) Mcp5, a meiotic cell cortex protein, is required for nuclear movement mediated by dynein and microtubules in fission yeast. J Cell Biol 173:27–33
Schuchert P, Langsford M, Käslin E, Kohli J (1991) A specific DNA sequence is required for high frequency of recombination in the ade6 gene of fission yeast. EMBO J 10:2157–2163
Shimanuki M, Miki F, Ding D-Q, Chikashige Y, Hiraoka Y, Horio T, Niwa O (1997) A novel fission yeast gene, kms1 +, is required for the formation of meiotic prophase-specific nuclear architecture. Mol Gen Genet 254:238–249
Smith GR (2001) Homologous recombination near and far from DNA breaks: Alternative roles and contrasting views. Annu Rev Genet 35:243–274
Smith GR, Boddy MN, Shanahan P, Russell P (2003) Fission yeast Mus81⋅Eme1 Holliday junction resolvase is required for meiotic crossing over but not for gene conversion. Genetics 165:2289–2293
Smith KN, Penkner A, Ohta K, Klein F, Nicolas A (2001) B-type cyclins CLB5 and CLB6 control the initiation of recombination and synaptonemal complex formation in yeast meiosis. Curr Biol 11:88–97
Steiner WW, Smith GR (2005a) Natural meiotic recombination hot spots in the Schizosaccharomyces pombe genome successfully predicted from the simple sequence motif M26. Mol Cell Biol 25:9054–9062
Steiner WW, Smith GR (2005b) Optimizing the nucleotide sequence of a meiotic recombination hotspot in Schizosaccharomyces pombe. Genetics 169:1973–1983
Steiner WW, Schreckhise RW, Smith GR (2002) Meiotic DNA breaks at the S. pombe recombination hotspot M26. Mol Cell 9:847–855
Stewart E, Chapman CR, Al-Khodairy F, Carr AM, Enoch T (1997) rqh1 +, a fission yeast gene related to the Bloom's and Werner's syndrome genes, is required for reversible S phase arrest. EMBO J 16:2682–2692
Sun H, Treco D, Schultes NP, Szostak JW (1989) Double-strand breaks at an initiation site for meiotic gene conversion. Nature 338:87–90
Szankasi P, Smith GR (1995) A role for exonuclease I from S. pombe in mutation avoidance and mismatch correction. Science 267:1166–1169
Szankasi P, Heyer WD, Schuchert P, Kohli J (1988) DNA sequence analysis of the ade6 gene of Schizosaccharomyces pombe: Wild-type and mutant alleles including the recombination hotspot allele ade6-M26. J Mol Biol 204:917–925
Szostak JW, Orr-Weaver TL, Rothstein RJ, Stahl FW (1983) The double-strand-break repair model for recombination. Cell 33:25–35
Tanaka K, Hao Z, Kai M, Okayama H (2001) Establishment and maintenance of sister chromatid cohesion in fission yeast by a unique mechanism. EMBO J 20:5779–5790
Tange Y, Horio T, Shimanuki M, Ding DQ, Hiraoka Y, Niwa O (1998) A novel fission yeast gene, tht1 +, is required for the fusion of nuclear envelopes during karyogamy. J Cell Biol 140:247–258
Tavassoli M, Shayeghi M, Nasim A, Watts FZ (1995) Cloning and characterization of the Schizosaccharomyces pombe rad32 gene: a gene required for repair of double strand breaks and recombination. Nucleic Acids Res 23:383–388
Tonami Y, Murakami H, Shirahige K, Nakanishi M (2005) A checkpoint control linking meiotic S phase and recombination initiation in fission yeast. Proc Natl Acad Sci USA 102:5797–5801
Tornier C, Bessone S, Varlet I, Rudolph C, Darmon M, Fleck O (2001) Requirement for Msh6, but not for Swi4 (Msh3), in Msh2-dependent repair of base-base mismatches and mononucleotide loops in Schizosaccharomyces pombe. Genetics 158:65–75
Usui T, Ohta T, Oshiumi H, Tomizawa J, Ogawa H, Ogawa T (1998) Complex formation and functional versatility of Mre11 of budding yeast in recombination. Cell 95:705–716
van den Bosch M, Zonneveld JB, Vreeken K, de Vries FA, Lohman PH, Pastink A (2002) Differential expression and requirements for Schizosaccharomyces pombe RAD52 homologs in DNA repair and recombination. Nucleic Acids Res 30:1316–1324
Virgin JB, Bailey JP (1998) The M26 hotspot of Schizosaccharomyces pombe stimulates meiotic ectopic recombination and chromosomal rearrangements. Genetics 149:1191–1204
Wahls WP, Smith GR (1994) A heteromeric protein that binds to a meiotic homologous recombination hotspot: correlation of binding and hotspot activity. Genes Dev 8:1693–1702
Wang SW, Read RL, Norbury CJ (2002) Fission yeast Pds5 is required for accurate chromosome segregation and for survival after DNA damage or metaphase arrest. J Cell Sci 115:587–598
Wood V, Gwilliam R, Rajandream M-A, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D et al. (2002) The genome sequence of Schizosaccharomyces pombe. Nature 415:871–880
Yamada T, Mizuno K, Hirota K, Kon N, Wahls WP, Hartsuiker E, Murofushi H, Shibata T, Ohta K (2004) Roles of histone acetylation and chromatin remodeling factor in a meiotic recombination hotspot. EMBO J 23:1792–1803
Yamamoto A, West RR, McIntosh JR, Hiraoka Y (1999) A cytoplasmic dynein heavy chain is required for oscillatory nuclear movement of meiotic prophase and efficient meiotic recombination in fission yeast. J Cell Biol 145:1233–1249
Yamashita A, Yamamoto M (2006) Fission yeast Num1p is a cortical factor anchoring dynein and is essential for the horse-tail nuclear movement during meiotic prophase. Genetics 173:1187–1196
Yokobayashi S, Yamamoto M, Watanabe Y (2003) Cohesins determine the attachment manner of kinetochores to spindle microtubules at meiosis I in fission yeast. Mol Cell Biol 23:3965–3973
Young JA, Schreckhise RW, Steiner WW, Smith GR (2002) Meiotic recombination remote from prominent DNA break sites in S. pombe. Mol Cell 9:253–263
Young JA, Hyppa RW, Smith GR (2004) Conserved and nonconserved proteins for meiotic DNA breakage and repair in yeasts. Genetics 167:593–605
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Cromie, G., Smith, G.R. (2007). Meiotic Recombination in Schizosaccharomyces pombe: A Paradigm for Genetic and Molecular Analysis. In: Egel, R., Lankenau, DH. (eds) Recombination and Meiosis. Genome Dynamics and Stability, vol 3. Springer, Berlin, Heidelberg. https://doi.org/10.1007/7050_2007_025
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