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Genome Integrity pp 267–283Cite as

The Role of Chromatin Structure and Nuclear Architecture in the Cellular Response to DNA Double-Strand Breaks

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Part of the book series: Genome Dynamics and Stability ((GENOME,volume 1))

Abstract

The processes involved in the repair of DNA double-strand breaks (DSB) were thoroughly investigated at the genetic and biochemical level. The importance of chromatin structure and nuclear architecture in the outcome of repair has, however, only slowly emerged. Important recent developments in the field comprise the characterization of passive and active chromatin alterations following DSB induction, the function of chromatin modifying complexes and chromatin remodeling complexes in DSB processing, and the role of histone H2AX in the recruitment of these complexes. In addition, the development of new technologies, such as in vivo fluorescence labeling of chromatin and proteins, and the localized induction of breaks by use of endonucleases and microirradiation, has allowed insights to be gained into the mobility of damaged chromatin regions in the nucleus.

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Acknowledgments

Work in the author's laboratory is supported by grants from Bundesamt für Strahlenschutz, Bundesministerium für Bildung und Forschung, and European Science Foundation. I thank all lab members and my collaborators for discussions and ideas. I apologize to all those whose work could not be cited for reasons of space and focus.

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  1. Radiobiological Institute, University of Munich, Schillerstr. 42, 80336, Munich, Germany

    Anna A. Friedl

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Dirk-Henner Lankenau

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Friedl, A.A. (2005). The Role of Chromatin Structure and Nuclear Architecture in the Cellular Response to DNA Double-Strand Breaks. In: Lankenau, DH. (eds) Genome Integrity. Genome Dynamics and Stability, vol 1. Springer, Berlin, Heidelberg . https://doi.org/10.1007/7050_001

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