Bio-electro-Fenton: A New Combined Process – Principles and Applications

  • Hugo Olvera-Vargas
  • Clément Trellu
  • Nihal Oturan
  • Mehmet A. OturanEmail author
Part of the The Handbook of Environmental Chemistry book series (HEC, volume 61)


Biological treatments show insufficient removal efficiency in the case of recalcitrant organic compounds. Therefore, the necessity of upgrading wastewater treatment plants (WWTPs) with advanced treatment steps is unequivocal. Advanced oxidation processes (AOPs) are the most effective technologies for the removal of a large range of organic pollutants from water due to the generation of strong oxidizing species like hydroxyl radicals (OH). However, AOPs often involve high energy and/or reagent consumption and are considered as less cost-effective than biological processes. Hence, the combination of AOPs and biological treatments has been implemented aiming at maximizing efficient removal of recalcitrant organic pollutants while minimizing treatment costs. Among AOPs, electrochemical advanced oxidation processes (EAOPs) have been widely explored during coupled processes, since they possess remarkable advantages, such as high efficiencies, operability at mild conditions, economic feasibility, ease of automation, as well as eco-friendly character. The electro-Fenton process (EF) stands out as one of the most applied EAOPs and the present chapter is devoted to the advances and applications of EF process as a treatment step coupled with biological methods: the so-called bio-electro-Fenton (Bio-EF) process, which brings together the high oxidation power of EF and cost-effectiveness of biological methods.


Biodegradability Bio-electro-Fenton Biological treatment By-products Combined process Electro-Fenton Hydroxyl radicals Mineralization Toxicity Water treatment 


  1. 1.
    Ribeiro AR, Nunes OC, Pereira MFR, Silva AMT (2015) An overview on the advanced oxidation processes applied for the treatment of water pollutants defined in the recently launched directive 2013/39/EU. Environ Int 75:33–51CrossRefGoogle Scholar
  2. 2.
    Uribe IO, Mosquera-Corral A, Rodicio JL, Esplugas S (2015) Advanced technologies for water treatment and reuse. AICHE J 61:3146–3158CrossRefGoogle Scholar
  3. 3.
    Barbosa MO, Moreira NFF, Ribeiro AR et al (2016) Occurrence and removal of organic micropollutants: an overview of the watch list of EU decision 2015/495. Water Res 94:257–279CrossRefGoogle Scholar
  4. 4.
    Luo Y, Guo W, Ngo HH et al (2014) A review on the occurrence of micropollutants in the aquatic environment and their fate and removal during wastewater treatment. Sci Total Environ 473–474:619–641CrossRefGoogle Scholar
  5. 5.
    Prasse C, Stalter D, Schulte-Oehlmann U et al (2015) Spoilt for choice: a critical review on the chemical and biological assessment of current wastewater treatment technologies. Water Res 87:237–270CrossRefGoogle Scholar
  6. 6.
    Ahmed MB, Zhou JL, Ngo HH et al (2016) Progress in the biological and chemical treatment technologies for emerging contaminant removal from wastewater: a critical review. J Hazard Mater 323:274–298CrossRefGoogle Scholar
  7. 7.
    Tran NH, Urase T, Ngo HH et al (2013) Insight into metabolic and cometabolic activities of autotrophic and heterotrophic microorganisms in the biodegradation of emerging trace organic contaminants. Bioresour Technol 146:721–731CrossRefGoogle Scholar
  8. 8.
    Fatta-Kassinos D, Kalavrouziotis IK, Koukoulakis PH, Vasquez MI (2011) The risks associated with wastewater reuse and xenobiotics in the agroecological environment. Sci Total Environ 409:3555–3563CrossRefGoogle Scholar
  9. 9.
    Picó Y, Barceló D (2015) Transformation products of emerging contaminants in the environment and high-resolution mass spectrometry: a new horizon. Anal Bioanal Chem 407:6257–6273CrossRefGoogle Scholar
  10. 10.
    Garcia-Rodríguez A, Matamoros V, Fontàs C, Salvadó V (2013) The ability of biologically based wastewater treatment systems to remove emerging organic contaminants – a review. Environ Sci Pollut Res 21:11708–11728CrossRefGoogle Scholar
  11. 11.
    Pomiès M, Choubert J-M, Wisniewski C, Coquery M (2013) Modelling of micropollutant removal in biological wastewater treatments: a review. Sci Total Environ 443:733–748CrossRefGoogle Scholar
  12. 12.
    Oturan MA, Aaron J-J (2014) Advanced oxidation processes in water/wastewater treatment: principles and applications. A review. Crit Rev Environ Sci Technol 44:2577–2641CrossRefGoogle Scholar
  13. 13.
    Oller I, Malato S, Sánchez-Pérez JA (2011) Combination of advanced oxidation processes and biological treatments for wastewater decontamination – a review. Sci Total Environ 409:4141–4166CrossRefGoogle Scholar
  14. 14.
    Dirany A, Sirés I, Oturan N et al (2012) Electrochemical treatment of the antibiotic sulfachloropyridazine: kinetics, reaction pathways, and toxicity evolution. Environ Sci Technol 46:4074–4082CrossRefGoogle Scholar
  15. 15.
    Oturan MA, Pimentel M, Oturan N, Sirés I (2008) Reaction sequence for the mineralization of the short-chain carboxylic acids usually formed upon cleavage of aromatics during electrochemical Fenton treatment. Electrochim Acta 54:173–182CrossRefGoogle Scholar
  16. 16.
    Ganzenko O, Huguenot D, van Hullebusch ED et al (2014) Electrochemical advanced oxidation and biological processes for wastewater treatment: a review of the combined approaches. Environ Sci Pollut Res 21:8493–8524CrossRefGoogle Scholar
  17. 17.
    Olvera-Vargas H, Oturan N, Buisson D, Oturan MA (2016) A coupled bio-EF process for mineralization of the pharmaceuticals furosemide and ranitidine: feasibility assessment. Chemosphere 155:606–613CrossRefGoogle Scholar
  18. 18.
    Trellu C, Ganzenko O, Papirio S et al (2016) Combination of anodic oxidation and biological treatment for the removal of phenanthrene and tween 80 from soil washing solution. Chem Eng J 306:588–596CrossRefGoogle Scholar
  19. 19.
    Ganzenko O, Trellu C, Papirio S et al (2017) Bio-electro-Fenton: evaluation of a combined biological-advanced oxidation treatment for pharmaceutical wastewater. Environ Sci Pollut Res. doi: 10.1007/s11356-017-8450-6
  20. 20.
    Pulgarin C, Invernizzi M, Parra S et al (1999) Strategy for the coupling of photochemical and biological flow reactors useful in mineralization of biorecalcitrant industrial pollutants. Catal Today 54:341–352CrossRefGoogle Scholar
  21. 21.
    Contreras S, Rodrıguez M, Momani FA et al (2003) Contribution of the ozonation pre-treatment to the biodegradation of aqueous solutions of 2,4-dichlorophenol. Water Res 37:3164–3171CrossRefGoogle Scholar
  22. 22.
    Brillas E, Sirés I, Oturan MA (2009) Electro-Fenton process and related electrochemical technologies based on Fenton’s reaction chemistry. Chem Rev 109:6570–6631CrossRefGoogle Scholar
  23. 23.
    Moreira FC, Boaventura RAR, Brillas E, Vilar VJP (2017) Electrochemical advanced oxidation processes: a review on their application to synthetic and real wastewaters. Appl Catal Environ 202:217–261CrossRefGoogle Scholar
  24. 24.
    Sopaj F, Oturan N, Pinson J et al (2016) Effect of the anode materials on the efficiency of the electro-Fenton process for the mineralization of the antibiotic sulfamethazine. Appl Catal Environ 199:331–341CrossRefGoogle Scholar
  25. 25.
    Mousset E, Ko ZT, Syafiq M et al (2016) Electrocatalytic activity enhancement of a graphene ink-coated carbon cloth cathode for oxidative treatment. Electrochim Acta 222:1628–1641CrossRefGoogle Scholar
  26. 26.
    Ganiyu SO, Le TXH, Bechelany M et al (2017) Hierarchical CoFe-layered double hydroxide modified carbon-felt cathode: synthesis, characterization and application in heterogeneous electro-Fenton degradation of organic pollutants at circumneutral pH. J Mater Chem A 4:17686–17693Google Scholar
  27. 27.
    He Z, Gao C, Qian M et al (2014) Electro-Fenton process catalyzed by Fe3O4 magnetic nanoparticles for degradation of C.I. Reactive blue 19 in aqueous solution: operating conditions, influence, and mechanism. Ind Eng Chem Res 53:3435–3447CrossRefGoogle Scholar
  28. 28.
    Khataee A, Sajjadi S, Hasanzadeh A et al (2017) One-step preparation of nanostructured martite catalyst and graphite electrode by glow discharge plasma for heterogeneous electro-Fenton like process. J Environ Manage 199:31–45CrossRefGoogle Scholar
  29. 29.
    Ganiyu SO, van Hullebusch ED, Cretin M et al (2015) Coupling of membrane filtration and advanced oxidation processes for removal of pharmaceutical residues: a critical review. Sep Purif Technol 156:891–914CrossRefGoogle Scholar
  30. 30.
    Martínez-Huitle CA, Rodrigo MA, Sirés I, Scialdone O (2015) Single and coupled electrochemical processes and reactors for the abatement of organic water pollutants: a critical review. Chem Rev 115:13362–13407CrossRefGoogle Scholar
  31. 31.
    Lin SH, Chang CC (2000) Treatment of landfill leachate by combined electro-Fenton oxidation and sequencing batch reactor method. Water Res 34:4243–4249CrossRefGoogle Scholar
  32. 32.
    Olvera-Vargas H, Cocerva T, Oturan N et al (2016) Bioelectro-Fenton: a sustainable integrated process for removal of organic pollutants from water: application to mineralization of metoprolol. J Hazard Mater 319:13–23CrossRefGoogle Scholar
  33. 33.
    Mousset E, Oturan N, van Hullebusch ED et al (2014) Treatment of synthetic soil washing solutions containing phenanthrene and cyclodextrin by electro-oxidation. Influence of anode materials on toxicity removal and biodegradability enhancement. Appl Catal Environ 160–161:666–675CrossRefGoogle Scholar
  34. 34.
    Mousset E, Oturan N, van Hullebusch ED et al (2014) Influence of solubilizing agents (cyclodextrin or surfactant) on phenanthrene degradation by electro-Fenton process – study of soil washing recycling possibilities and environmental impact. Water Res 48:306–316CrossRefGoogle Scholar
  35. 35.
    Mousset E et al. (2017) Soil remediation by electro-Fenton process. Handb Environ Chem. doi: 10.1007/698_2017_38
  36. 36.
    Belkheiri D, Fourcade F, Geneste F et al (2011) Feasibility of an electrochemical pre-treatment prior to a biological treatment for tetracycline removal. Sep Purif Technol 83:151–156CrossRefGoogle Scholar
  37. 37.
    Yahiaoui I, Aissani-Benissad F, Fourcade F, Amrane A (2013) Removal of tetracycline hydrochloride from water based on direct anodic oxidation (Pb/PbO2 electrode) coupled to activated sludge culture. Chem Eng J 221:418–425CrossRefGoogle Scholar
  38. 38.
    Grafias P, Xekoukoulotakis NP, Mantzavinos D, Diamadopoulos E (2010) Pilot treatment of olive pomace leachate by vertical-flow constructed wetland and electrochemical oxidation: an efficient hybrid process. Water Res 44:2773–2780CrossRefGoogle Scholar
  39. 39.
    Mansour D, Fourcade F, Soutrel I et al (2015) Mineralization of synthetic and industrial pharmaceutical effluent containing trimethoprim by combining electro-Fenton and activated sludge treatment. J Taiwan Inst Chem Eng 53:58–67CrossRefGoogle Scholar
  40. 40.
    Pignatello JJ, Oliveros E, MacKay A (2006) Advanced oxidation processes for organic contaminant destruction based on the Fenton reaction and related chemistry. Crit Rev Environ Sci Technol 36:1–84CrossRefGoogle Scholar
  41. 41.
    Mansour D, Fourcade F, Huguet S et al (2014) Improvement of the activated sludge treatment by its combination with electro Fenton for the mineralization of sulfamethazine. Int Biodeter Biodegr 88:29–36CrossRefGoogle Scholar
  42. 42.
    Moreira FC, Soler J, Fonseca A et al (2015) Incorporation of electrochemical advanced oxidation processes in a multistage treatment system for sanitary landfill leachate. Water Res 81:375–387CrossRefGoogle Scholar
  43. 43.
    Zhu X, Ni J (2009) Simultaneous processes of electricity generation and p-nitrophenol degradation in a microbial fuel cell. Electrochem Commun 11:274–277CrossRefGoogle Scholar
  44. 44.
    Huong Le TX, Esmilaire R, Drobek M et al (2016) Design of a novel fuel cell-Fenton system: a smart approach to zero energy depollution. J Mater Chem A 4:17686–17693CrossRefGoogle Scholar
  45. 45.
    Feng C-H, Li F-B, Mai H-J, Li X-Z (2010) Bio-electro-Fenton process driven by microbial fuel cell for wastewater treatment. Environ Sci Technol 44:1875–1880CrossRefGoogle Scholar
  46. 46.
    Yong X-Y, Gu D-Y, Wu Y-D et al (2017) Bio-electron-Fenton (BEF) process driven by microbial fuel cells for triphenyltin chloride (TPTC) degradation. J Hazard Mater 324:178–183CrossRefGoogle Scholar
  47. 47.
    Birjandi N, Younesi H, Ghoreyshi AA, Rahimnejad M (2016) Electricity generation through degradation of organic matters in medicinal herbs wastewater using bio-electro-Fenton system. J Environ Manage 180:390–400CrossRefGoogle Scholar
  48. 48.
    Wang X-Q, Liu C-P, Yuan Y, Li F (2014) Arsenite oxidation and removal driven by a bio-electro-Fenton process under neutral pH conditions. J Hazard Mater 275:200–209CrossRefGoogle Scholar
  49. 49.
    Ferrag-Siagh F, Fourcade F, Soutrel I et al (2014) Electro-Fenton pretreatment for the improvement of tylosin biodegradability. Environ Sci Pollut Res 21:8534–8542CrossRefGoogle Scholar
  50. 50.
    Gong Y, Li J, Zhang Y et al (2016) Partial degradation of levofloxacin for biodegradability improvement by electro-Fenton process using an activated carbon fiber felt cathode. J Hazard Mater 304:320–328CrossRefGoogle Scholar
  51. 51.
    Mansour D, Fourcade F, Soutrel I et al (2015) Relevance of a combined process coupling electro-Fenton and biological treatment for the remediation of sulfamethazine solutions – application to an industrial pharmaceutical effluent. Comptes Rendus Chim 18:39–44CrossRefGoogle Scholar
  52. 52.
    Ledezma Estrada A, Li Y-Y, Wang A (2012) Biodegradability enhancement of wastewater containing cefalexin by means of the electro-Fenton oxidation process. J Hazard Mater 227:41–48CrossRefGoogle Scholar
  53. 53.
    Moussavi G, Bagheri A, Khavanin A (2012) The investigation of degradation and mineralization of high concentrations of formaldehyde in an electro-Fenton process combined with the biodegradation. J Hazard Mater 237:147–152CrossRefGoogle Scholar
  54. 54.
    Vidal J, Huiliñir C, Salazar R (2016) Removal of organic matter contained in slaughterhouse wastewater using a combination of anaerobic digestion and solar photoelectro-Fenton processes. Electrochim Acta 210:163–170CrossRefGoogle Scholar
  55. 55.
    Cañizares P, Paz R, Sáez C, Rodrigo MA (2009) Costs of the electrochemical oxidation of wastewaters: a comparison with ozonation and Fenton oxidation processes. J Environ Manage 90:410–420CrossRefGoogle Scholar
  56. 56.
    Garcia-Segura S, Brillas E (2014) Advances in solar photoelectro-Fenton: decolorization and mineralization of the direct yellow 4 diazo dye using an autonomous solar pre-pilot plant. Electrochim Acta 140:384–395CrossRefGoogle Scholar
  57. 57.
    Mook WT, Aroua MK, Issabayeva G (2014) Prospective applications of renewable energy based electrochemical systems in wastewater treatment: a review. Renew Sustain Energy Rev 38:36–46CrossRefGoogle Scholar
  58. 58.
    Wang L, Cao M, Ai Z, Zhang L (2015) Design of a highly efficient and wide pH electro-Fenton oxidation system with molecular oxygen activated by ferrous-tetrapolyphosphate complex. Environ Sci Technol 49:3032–3039CrossRefGoogle Scholar
  59. 59.
    Zhang C, Zhou M, Yu X et al (2015) Modified iron-carbon as heterogeneous electro-Fenton catalyst for organic pollutant degradation in near neutral pH condition: characterization, degradation activity and stability. Electrochim Acta 160:254–262CrossRefGoogle Scholar
  60. 60.
    Le TXH, Bechelany M, Lacour S, Oturan N, Oturan MA, Cretin M (2015) High removal efficiency of dye pollutants by electron-Fenton process using a graphene based cathode. Carbon 94:1003–1011CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2017

Authors and Affiliations

  • Hugo Olvera-Vargas
    • 1
  • Clément Trellu
    • 1
  • Nihal Oturan
    • 1
  • Mehmet A. Oturan
    • 1
    Email author
  1. 1.Laboratoire Géomatériaux et Environnement (LGE), EA 4508Université Paris-Est, UPEMMarne-la-Vallée, Cedex 2France

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