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Bacterial Diversity of the Boka Kotorska Bay

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The Boka Kotorska Bay Environment

Part of the book series: The Handbook of Environmental Chemistry ((HEC,volume 54))

Abstract

This chapter provides an overview on the results of bacterial diversity studies conducted in the Boka Kotorska Bay using flow cytometry, epifluorescence microscopy as well as CARD FISH methods. Preliminary results of bacterial communities in sediment using next generation sequencing are presented as well. The Boka Kotorska Bay represents a semi-closed basin with specific hydrographic characteristics where it is very important to investigate bacterioplankton structure and highlight their significance. Average results of heterotrophic bacteria showed that Boka Kotorska Bay during 2009 and 2010 belonged to oligotrophic area. In the course of 2011 and 2012 Kotor and Risan represented mesoeutrophic area while Tivat, Herceg Novi and Mamula belonged to oligotrophic area. Analysis of seasonal distribution of LNA and HNA groups in the area of Boka Kotorska Bay showed prevalence of the LNA bacteria during spring, summer and autumn, while prevalence of the HNA bacterial group was occurred only in winter time. Bacterioplankton abundance obtained by CARD FISH showed variations among different part of Bay. Abundance of all bacterial groups decreased going from the inner (Kotor and Risan Bay) to the outer part (Herceg Novi and Mamula). In inner part of the Bay dominated Gammaproteobacteria and Cytophaga/Flavobacter, typical chemoorganotrophs. This part of investigated area was affected by inflow of fresh waters, nutrients as well as under great influence of contamination taking account bacterial faecal indicators. Preliminary results of sediment analysis showed dominance of Proteobacteria, Firmicutes, and Actinobacteria in surface sediment layers especially Gammaproteobacteria in the most polluted sites.

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Acknowledgement

These studies were supported by bilateral projects Montenegro-Croatia: Molecular and Physiological Diversity of the trophic gradients in the Adriatic Sea; Phylogenetic analysis of the diversity of bacterial communities in sediment of the Boka Kotorska Bay. Part of the research was supported by Croatian Science Foundation (BABAS project to SO).

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Authors and Affiliations

  1. Institute of Marine Biology, University of Montenegro, 69, Kotor, Montenegro

    Sandra Jokanović & Aleksandra Huter

  2. Institute Ruđer Bošković, Bijenička cesta 54, Zagreb, Croatia

    Sandi Orlić

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  1. Sandra Jokanović
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  2. Aleksandra Huter
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  3. Sandi Orlić
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Correspondence to Sandra Jokanović .

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Editors and Affiliations

  1. Institute of Marine Biology, University of Montenegro, Kotor, Montenegro

    Aleksandar Joksimović

  2. Institute of Marine Biology, University of Montenegro, Kotor, Montenegro

    Mirko Djurović

  3. S.Yu. Witte Moscow University, Moscow, Russia

    Aleksander V. Semenov

  4. Engineering Research Production Center for Water Management, Land Reclamation and Ecology “Soyuzvodproject”, Moscow, Russia

    Igor S. Zonn

  5. P.P. Shirshov Institute of Oceanology, Russian Academy of Sciences, Moscow, Russia

    Andrey G. Kostianoy

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Jokanović, S., Huter, A., Orlić, S. (2016). Bacterial Diversity of the Boka Kotorska Bay. In: Joksimović, A., Djurović, M., Semenov, A., Zonn, I., Kostianoy, A. (eds) The Boka Kotorska Bay Environment . The Handbook of Environmental Chemistry, vol 54. Springer, Cham. https://doi.org/10.1007/698_2016_32

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