Activity of Lysosomal Enzymes During Protein Malnutrition and Progesterone Supplementation in the Mouse

  • Iwona Stanisławska
  • Bożena Witek
  • Małgorzata Czarny-Działak
  • Ewa Pałka-Łebek
  • Marek ŁypEmail author
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1211)


This study investigated the effects of protein malnutrition and progesterone supplementation on the activities of a spectrum of lysosomal enzymes in tissue fragments of mouse liver and kidney. The working hypothesis was that the known anti-stress action of progesterone could have to do with the inhibition of lysosomes which are engaged in apoptotic and oxidative stress-induced responses. The study investigated the effects of exogenous progesterone in chronically (3 weeks) protein-malnourished (10% protein) mice on the activities of lysosomal hydrolases in liver and kidney tissues. Progesterone was injected intraperitoneally in a dose of 2 μg/g body mass dissolved in a vehicle volume of 10 μL/g body mass during the final 3 days of exposure to either low 10% or standard 16% protein content in the chow. After euthanizing the animals, tissue fragments of liver and kidney assayed for the content of lysosomal enzymes. The results demonstrated the stimulating effect of protein malnutrition on lysosomal activities. We further found, contrary to our hypothesis, that progesterone supplementation during both standard and low-protein conditions enhanced lysosomal activities, particularly acting in concert with protein malnutrition in kidney tissue. The effects were selective concerning both lysosomal enzymes and tissues and of highly variable magnitude. Nonetheless, we believe we have shown that progesterone assists protein malnutrition in stimulation of lysosomal enzymes, which suggests the possibility of the hormone’s engagement in cleansing the cellular milieu in disorders consisting of accumulation of toxic molecules.


Lysosomal enzymes Lysosomes Mice Progesterone Protein restriction 


Conflicts of Interest

The authors report no conflicts of interest in relation to this article.

Ethical Approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. The study protocol was approved by the Bioethics Commission of the Swiętokrzyska Medical Chamber (permit 47/2016).


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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Iwona Stanisławska
    • 1
  • Bożena Witek
    • 2
  • Małgorzata Czarny-Działak
    • 3
  • Ewa Pałka-Łebek
    • 4
  • Marek Łyp
    • 1
    • 5
    Email author
  1. 1.Department of DieteticsCollege of Rehabilitation in WarsawWarsawPoland
  2. 2.Institute of BiologyThe Jan Kochanowski UniversityKielcePoland
  3. 3.Faculty of Medicine and Health SciencesThe Jan Kochanowski UniversityKielcePoland
  4. 4.Faculty of Mathematics and Natural SciencesJan Kochanowski UniversityKielcePoland
  5. 5.Department of PhysiotherapyCollege of RehabilitationWarsawPoland

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