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Stem Cells from Human Extracted Deciduous Teeth Expanded in Foetal Bovine and Human Sera Express Different Paracrine Factors After Exposure to Freshly Prepared Human Serum

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Tissue Engineering and Regenerative Medicine

Part of the book series: Advances in Experimental Medicine and Biology ((ICRRM,volume 1084))

Abstract

Background: The response of stem cells to paracrine factors within the host’s body plays an important role in the regeneration process after transplantation. The aim of this study was to determine the viability and paracrine factor profile of stem cells from human extracted deciduous teeth (SHED) pre-cultivated in media supplemented with either foetal bovine serum (FBS) or pooled human serum (pHS) in the presence of individual human sera (iHS).

Methods: SHED (n = 3) from passage 4 were expanded in FBS (FBS-SHED) or pHS (pHS-SHED) supplemented media until passage 7. During expansion, the proliferation of SHED was determined. Cells at passage 7 were further expanded in human serum from four individual donors (iHS) for 120 h followed by assessment of cell viability and profiling of the secreted paracrine factors.

Results: Proliferation of SHED was significantly higher (p < 0.05) in pHS supplemented media compared to FBS supplemented media. pHS-SHED also maintained their higher proliferation rate compared to FBS-SHED in the presence of iHS. In iHS supplemented media, FBS-SHED expressed significantly higher levels of SDF-1A (p < 0.05) after 24 h compared to pHS-SHED. Similar results were found for HGF (p < 0.01), LIF (p < 0.05), PDGF-BB (p < 0.05), SDF-1A (p < 0.01), and IL-10 (p < 0.05) when cell culture supernatants from FBS-SHED were profiled 120 h post-incubation.

Conclusion: SHED expanded in pHS instead of FBS have higher proliferative capacity and show an altered secretion profile. Further studies are needed to determine whether these differences could result in better engraftment and regeneration following transplantation.

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Abbreviations

CPD:

cumulative population doubling time

FBS:

foetal bovine serum

FGF-2:

fibroblast growth factor 2

G-CSF:

granulocyte colony stimulating factor

HGF:

hepatocyte growth factor

IGF-1:

insulin like growth factor 1

iHS:

individual human sera

IL:

interleukin

ISCT:

International Society for Cellular Therapy

LIF:

leukaemia inhibitory factor

MSCs:

mesenchymal stem cells

PDGF-BB:

platelet-derived growth factor BB

pHS:

pooled human serum

SCF:

stem cell factor

SDF-1A:

stromal cell-derived factor-1A

SHED:

stem cells from human extracted deciduous teeth

VEGF:

vascular endothelial growth factor

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Acknowledgement

This work was supported by High Impact Research MOHE Grant UM.C/625/1/HIR/MOHE/DENT/01 from the Ministry of Higher Education Malaysia and University of Malaya Research Grant UMRG RP019/13HTM. The authors thank the staff nurses from the Oro-Maxillofacial Surgical and Medical Sciences Department, Faculty of Dentistry, University of Malaya, for their support in collection of blood from donors.

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The authors declare no conflicts of interest.

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Authors and Affiliations

  1. Department of Oral Biology and Biomedical Sciences, Faculty of Dentistry, MAHSA University, Jenjarum, Selangor, Malaysia

    Nazmul Haque

  2. Regenerative Dentistry Research Group, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia

    Nazmul Haque & Noor Hayaty Abu Kasim

  3. Stem Cell Biology and Regenerative Medicine Group, School of Pharmacy, University of Reading, Reading, UK

    Darius Widera

  4. Department of Restorative Dentistry, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia

    Noor Hayaty Abu Kasim

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  1. Nazmul Haque
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Correspondence to Noor Hayaty Abu Kasim .

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  1. Laboratory of Stem Cell Research and Application, University of Science, Vietnam National University, Ho Chi Minh City, Vietnam

    Phuc Van Pham

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Haque, N., Widera, D., Abu Kasim, N.H. (2018). Stem Cells from Human Extracted Deciduous Teeth Expanded in Foetal Bovine and Human Sera Express Different Paracrine Factors After Exposure to Freshly Prepared Human Serum. In: Pham, P. (eds) Tissue Engineering and Regenerative Medicine. Advances in Experimental Medicine and Biology(), vol 1084. Springer, Cham. https://doi.org/10.1007/5584_2018_299

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