Advertisement

Circulation of Influenza Virus in the 2015/2016 Epidemic Season in Poland: Serological Evaluation of Anti-hemagglutinin Antibodies

  • D. KowalczykEmail author
  • K. Szymański
  • K. Cieślak
  • E. Hallmann-Szelińska
  • L. B. Brydak
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1150)

Abstract

The diagnostic of influenza virus infections is possible using molecular biology methods as well as the analysis of anti-hemagglutinin (anti-HA) antibodies in the blood serum. The aim of this study was to determine the level of anti-HA antibodies in 7 age groups of patients during the 2015/2016 epidemic season in Poland. A total of 1050 serum samples were tested using the hemagglutination inhibition (HAI) assay. We confirmed the presence of anti-HA antibodies for the influenza virus strains: A/California/7/2009(H1N1)pdm09, A/Switzerland/9715293/2013(H3N2), and B/Phuket/3073/2013, which were the components of the influenza vaccine for the 2015/2016 epidemic season. The level of specific anti-HA antibodies was different in each age group. The geometric mean titers were highest at age 5–9 years, where the antibody protection level reached 61.3% against B/Phuket/3073/2013 and 52.7% for A/Switzerland/9715293/2013(H3N2) antigen. The antibody level amounted to 56.7% against for antigen B at age 45–64. In the remaining age groups, the protection levels for all hemagglutinin types did not exceed 50%. These findings confirm the urgent need to increase a persistently low influenza vaccination coverage in the Polish population, which may have had its part in the noticeable increase in the confirmed cases of influenza and influenza-like virus infection during the season.

Keywords

Antihemagglutinin antibodies Epidemic season Hemagglutination inhibition assay Infection Influenza Rate of protection 

Notes

Acknowledgments

Funded by NIPH–NIH research grant 3/EM. We acknowledge physicians and employees of Voivodship Sanitary Epidemiologic Stations, participating in the SENTINEL and NON-SENTINEL programs for their input into the influenza surveillance in Poland. We would like to thank the Malec Company for providing fertilized chicken embryos.

Conflicts of Interest

The authors declare no conflicts of interest in relation to this article.

References

  1. Bednarska K, Nowak M, Kondratiuk K, Hallmann-Szelińska E, Brydak LB (2015) Incidence of circulating antibodies against hemagglutinin of influenza viruses in the epidemic sesason 2013/2014 in Poland. Adv Exp Med Biol 857:45–40CrossRefGoogle Scholar
  2. Bednarska K, Hallmann-Szelińska E, Kondratiuk K, Brydak LB (2016) Antigenic drift of A/H3N2 virus and circulation of influenza-like viruses during the 2014/2015 influenza season in Poland. Adv Exp Med Biol 905:33–38CrossRefGoogle Scholar
  3. Brydak LB, Nitsch-Osuch A (2014) Prevention of influenza infection – a Polish perspective. Postepy Hig Med Dosw (Online) 3:137–144CrossRefGoogle Scholar
  4. Cieślak K, Szymański K, Kowalczyk D, Brydak LB (2017) Influenza and influenza-like viruses in children in the epidemic season 2015/2016 in Poland. Adv Exp Med Biol 968:13–18CrossRefGoogle Scholar
  5. Ekiert DC, Friesen RH, Bhabha G, Kwaks T, Jongeneelen M, Yu W, Ophorst C, Cox F, Korse HJ, Brandenburg B, Vogels R, Brakenhoff JP, Kompier R, Koldijk MH, Cornelissen LA, Poon LL, Peiris M, Koudstaal W, Wilson IA, Goudsmit J (2011) A highly conserved neutralizing epitope on group 2 influenza A viruses. Science 333(6044):843–850CrossRefGoogle Scholar
  6. Hallmann-Szelińska E, Bednarska K, Kondratiuk K, Rabczenko D, Brydak LB (2016a) Viral infections in children in the 2014/2015 epidemic season in Poland. Adv Exp Med Biol 912:51–56CrossRefGoogle Scholar
  7. Hallmann-Szelińska E, Bednarska K, Korczyńska M, Paradowska-Stankiewicz I, Brydak LB (2016b) Virological characteristics of the 2014/2015 influenza season based on molecular analysis of biological material derived from I-MOVE Study. Adv Exp Med Biol 921:81–85CrossRefGoogle Scholar
  8. Hallmann-Szelińska E, Cieślak K, Kowalczyk D, Szymański K, Brydak LB (2018) Antibodies to influenza virus Hemagglutinin in the 2016/2017 epidemic season in Poland. Adv Exp Med Biol.  https://doi.org/10.1007/5584_2018_232 (Epub ahead of print)Google Scholar
  9. Kowalczyk D, Szymański K, Cieślak K, Brydak LB (2017a) Serlogical characteristic of antibodies against hemaglutynin of influenza viruses circulated in 2014–2015 epidemic season in Poland. Adv Exp Med Biol 968:35–40CrossRefGoogle Scholar
  10. Kowalczyk D, Cieślak K, Szymański K, Brydak LB (2017b) The activity of influenza and influenza-like viruses in individuals aged over 14 in the 2015/2016 influenza season in Poland. Adv Exp Med Biol 980:45–50CrossRefGoogle Scholar
  11. Mandelboim M, Bromberg M, Sherbany H, Zucker I, Yaary K, Bassal R, Dichtiar R, Cohen D, Shohat T, Mendelson E, Green MS (2014) Significant cross reactive antibodies to influenza virus in adults and children during a period of marked antigenic drift. BMC Infect Dis 14:346CrossRefGoogle Scholar
  12. Nichol KL (2008) Efficacy and effectiveness of influenza vaccination. Vaccine 26(Suppl 4):D17–D22CrossRefGoogle Scholar
  13. NIPH-NIH Report (2016) Epimeld. Influenza and influenza-like illness in Poland. http://wwwold.pzh.gov.pl/oldpage/epimeld/grypa/index.htm. Accessed on 24 July 2017
  14. Szymanski K, Kowalczyk D, Cieślak K, Brydak LB (2017) Regional diversification of influenza activity in Poland during the 2015/16 epidemic season. Adv Exp Med Biol 1020:1–6CrossRefGoogle Scholar
  15. Throsby M, van den Brink E, Jongeneeln M, Poon LL, Alard P, Cornelissen L, Bakker A, Cox F, van Deventer E, Guan Y, Cinatl J, ter Meulen J, Lasters I, Carsetti R, Peiris M, de Kruif J, Goudsmit J (2008) Heterosubtypic neutralizing monoclonal antibodies cross-protective against H5N1 and H1N1 recovered from human IgM+ memory B cells. PLoS One 3(12):e3942CrossRefGoogle Scholar
  16. WHO (2011) Manual for the laboratory diagnosis and virological surveillance of influenza. WHO Press, Geneva, pp 1–153Google Scholar
  17. Wu Y, Wu Y, Tefsen B, Shi Y, Gao GF (2014) Bat-derived influenza-like viruses H17N10 and H18N11. Trends Microbiol 22:183–191CrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG  2018

Authors and Affiliations

  • D. Kowalczyk
    • 1
    Email author
  • K. Szymański
    • 1
  • K. Cieślak
    • 1
  • E. Hallmann-Szelińska
    • 1
  • L. B. Brydak
    • 1
  1. 1.Department of Influenza Research, National Influenza CenterNational Institute of Public Health – National Institute of HygieneWarsawPoland

Personalised recommendations