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Paraoxonase 1 Gene L55M Polymorphism and Paraoxonase 1 Activity in Obstructive Sleep Apnea Patients

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Pulmonary Health and Disorders

Part of the book series: Advances in Experimental Medicine and Biology ((NR,volume 1150))

Abstract

The antioxidant enzyme paraoxonase-1 (PON1) may limit oxidative stress in the development of cardiovascular diseases (CVD) and obstructive sleep apnea (OSA). The aim of the study was to determine PON1 gene L55M polymorphism in OSA-positive and OSA-negative subjects, along with paraoxonase activity of the enzyme (PON1-act). Caucasians aged 25–75, with BMI 19.0–53.0 kg/m2 and no acute or severe chronic disorder underwent polysomnography, and OSA-negative (n = 44) and OSA-positive (n = 57) groups were established. The following parameters were assessed: arterial blood pressure and serum glucose, lipids, C–reactive protein, and homocysteine. Genomic DNA was extracted and amplified, and automatic sequencing was used to confirm the LL, LM, MM genotypes. PON1–act was measured spectrophotometrically using paraoxon as a substrate. We found that frequency of polymorphisms differed significantly between the OSA–negative and OSA–positive patients (p < 0.05). Increased PON1–act was observed in the LL–genotype versus the LM + MM–genotype in the study population (p < 0.05). PON1–act was higher in the OSA–negative compared with OSA–positive patients (p < 0.001); in general and in the subgroups presenting the LL or LM genotype. In addition, there was an inverse relationship between PON1–act and LDL–cholesterol in the entire study population. The OSA–positive group presented an inverse relationship between PON1–act and fasting glucose. We conclude that patients could benefit from the LL genotype related with higher activity of PON1. OSA pathology might decrease the enzyme activity, despite the presence of L allele.

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Conflicts of Interest

The authors declare no conflicts of interests in relation to this work.

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Authors and Affiliations

  1. Department of Clinical Biochemistry and Laboratory Medicine, Poznan University of Medical Sciences, Poznań, Poland

    A. Płóciniczak & D. Formanowicz

  2. Department of Laboratory Diagnostics, Poznan University of Medical Sciences, Poznań, Poland

    A. Baszczuk, A. Ludziejewska, G. Kasprzak & E. Wysocka

  3. Department of Respiratory Medicine, Allergology and Pulmonary Oncology, Poznan University of Medical Sciences, Poznań, Poland

    H. Winiarska & S. Cofta

  4. Department of Neurochemistry and Neuropathology, Poznan University of Medical Sciences, Poznań, Poland

    S. Michalak

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  1. A. Płóciniczak
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  2. A. Baszczuk
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  3. A. Ludziejewska
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  4. H. Winiarska
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  7. D. Formanowicz
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  8. S. Cofta
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  9. E. Wysocka
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Correspondence to E. Wysocka .

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  1. Opole Medical School, Opole, Poland

    Mieczyslaw Pokorski

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Płóciniczak, A. et al. (2018). Paraoxonase 1 Gene L55M Polymorphism and Paraoxonase 1 Activity in Obstructive Sleep Apnea Patients. In: Pokorski, M. (eds) Pulmonary Health and Disorders. Advances in Experimental Medicine and Biology(), vol 1150. Springer, Cham. https://doi.org/10.1007/5584_2018_267

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