Gene Therapy Strategies in Bone Tissue Engineering and Current Clinical Applications

Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1119)


Gene therapy provides a promising approach for regeneration and repair of injured bone. Application of gene therapy has displayed increased efficiency in various animal models and preclinical trials in comparison with traditional bone grafting methods. The objective of this review is to highlight fundamental principles of gene therapy strategies in bone tissue engineering and solutions of their current limitations for the healing of bone injury. Vector types are debated for the repair of defected site due to demonstration of constraints and applications of the protocols. In recent years, the combination of gene therapy strategies and bone tissue engineering has highly gained attention. We discussed viral and non-viral mediated delivery of therapeutic protein by using scaffolds for bone tissue engineering. Although pre-clinical studies have showed that gene therapy has very promising results to heal injured bone, there are several limitations regarding with the usage of gene delivery methods into clinical applications. Choice of suitable vector, selection of transgene and gene delivery protocols are the most outstanding questions. This article also addresses current state of gene delivery strategies in bone tissue engineering for their potential applications in clinical considerations.


Bone tissue engineering Gene activated matrices Gene therapy Viral vectors 



Adeno-associated virus


Adipose stem cell


Bone morphogenetic proteins;


Complementary deoxyribonucleic acid


Type I collagen


Double-stranded DNA


the US Food and Drug Administration


Gene-activated matrices


Good manufacturing practice




Human immunodeficiency virus


Insulin-like growth factor








LIM mineralization protein-1


Moloney murine leukemia virus


Mesenchymal stem cells




Platelet-derived growth factor


Poly (ethylene glycol)


Poly (lactide-co-glycolic acid)


Parathyroid hormone


Receptor activator of nuclear factor kappa-B ligand


Runt related transcription factor 2


X-linked severe combined immunodeficiency disease


Single stranded-DNA

TGF- β

Transforming growth factor-β


Tumor necrosis factor-α


Vascular endothelial growth factor


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© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Faculty of Engineering, Genetics and Bioengineering DepartmentYeditepe UniversityIstanbulTurkey
  2. 2.Biotechnology Research LaboratoryCenter of Excellence in Biomaterials and Tissue Engineering, BIOMATEN, METUAnkaraTurkey

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