Intravenous Infusion of Human Adipose Tissue-Derived Mesenchymal Stem Cells to Treat Type 1 Diabetic Mellitus in Mice: An Evaluation of Grafted Cell Doses

  • Loan Thi-Tung Dang
  • Anh Nguyen-Tu Bui
  • Cong Le-Thanh Nguyen
  • Nhat Chau Truong
  • Anh Thi-Van Bui
  • Ngoc Phan Kim
  • Kiet Dinh Truong
  • Phuc Van PhamEmail author
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1083)


Mesenchymal stem cell (MSC) transplantation is a novel treatment for diabetes mellitus, especially type 1 diabetes. Many recent publications have demonstrated the efficacy of MSC transplantation on reducing blood glucose and increasing insulin production in both preclinical and clinical trials. However, the investigation of grafted cell doses has been lacking. Therefore, this study aimed to evaluate the different doses of MSCs on treatment of type 1 diabetes in mouse models. MSCs were isolated and expanded from human adipose tissue. Streptozotocin (STZ)-induced diabetic mice were divided into two groups that were intravenously transfused with two different doses of human MSCs: 106 or 2.106 cells/mouse. After transplantation, both grafted and placebo mice were monitored weekly for their blood glucose levels, glucose and insulin tolerance, pancreatic structural changes, and insulin production for 56 days after transplantation. The results showed that the higher dose of MSCs (2.106 cells/mouse) remarkably reduced death rate. The death rates were 50%, 66%, and 0% in placebo group, low-dose (1.106 MSCs) group, and high-dose (2.106 MSCs) group, respectively, after 56 days of treatment. Moreover, blood glucose levels were lower for the high-dose group compared to other groups. Glucose and insulin tolerance, as well as insulin production, were significantly improved in mice transplanted with 2.106 cells. The histochemical analyses also support these results. Thus, a higher (e.g., 2.106) dose of MSCs may be an effective dose for treatment of type 1 diabetes mellitus.


Diabetes mellitus Stem cells Adipose-derived stem cells Mesenchymal stem cells Cell dose Islet regeneration 



Human adipose-derived stem cells


Glucose tolerance test


Hematoxylin and eosin


Mesenchymal stem cells




T helper 1/T helper 2


Tumor necrosis factor-alpha








Epidermal growth factor


Basic fibroblast growth factor


Platelet-derived growth factor


Transforming growth factor-beta


Vascular endothelial growth factor


Hepatocyte growth factor


Insulin growth factor-1


Nitric oxide


Prostaglandin E2


Indoleamine 2,3-dioxygenase



This research was funded by the Ministry of Science and Technology via project Grant No. DTDL.2012-G/23 and Vietnam National University, Ho Chi Minh City, via project No. C2016-18-18.

Author Contribution

LTTD, designed the study, performed the experiments, analyzed the data, and drafted the manuscript; PVP, KDT, designed the study and reviewed and corrected the manuscript; ANTB, CLTN, performed the experiments and analyzed the data and drafted the manuscript; NCT, ATVB, performed the experiments and reviewed and drafted the manuscript; and NPK analyzed the data and wrote the manuscript.

Competing Interests

The authors declare that no competing interests exist.


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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  • Loan Thi-Tung Dang
    • 1
  • Anh Nguyen-Tu Bui
    • 1
  • Cong Le-Thanh Nguyen
    • 1
    • 2
  • Nhat Chau Truong
    • 1
    • 2
  • Anh Thi-Van Bui
    • 1
    • 2
  • Ngoc Phan Kim
    • 1
  • Kiet Dinh Truong
    • 3
  • Phuc Van Pham
    • 1
    • 2
    Email author
  1. 1.Stem Cell InstituteUniversity of Science, VNUHCMHo Chi Minh cityVietnam
  2. 2.Laboratory of Stem Cell Research and ApplicationUniversity of Science, VNUHCMHo Chi Minh cityVietnam
  3. 3.Medical Genetics InstituteHo Chi Minh cityVietnam

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