Abstract
The guinea pig sensitized by ovalbumin is the most widely used model to study cough experimentally, as the neurophysiology of the vagus nerve in the guinea pig is closest to humans. Nonetheless, the choice of the antigen remains questionable, which influences the translation of results into clinical medicine. The present study seeks to develop an alternative model of cough study using house dust mite sensitization (HDM). Thirty guinea pigs were divided into the HDM group, ovalbumin (OVA) group, and control group based on their cough response to 0.4 M citric acid. In the HDM group animals were sensitized by 0.25 %HDM aerosol, which they inhaled for 5 min over 5 days, followed by inhalation of 0.5 %HDM in the same protocol. Sensitization was confirmed by a skin test. Symptoms of allergic rhinitis were induced by intranasal application of 15 μl 0.5 %HDM and cough challenges with citric acid were performed. Airway resistance was measured in vivo by Pennock’s method. We found that both HDM and OVA-sensitized groups showed a significantly enhanced nasal reactivity and cough response compared with controls. The airway resistance data did not show significant differences. We conclude that the HDM cough model replicates functional aspects of the OVA model, which may make it an alternative to the latter. However, the superiority of the HDM model for experimental cough studies remains to be further explored.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Belvisi MG, Bolser DC (2002) Summary: animal models for cough. Pulm Pharmacol Ther 15(3):249–250
Bhure UN, Bhure SU, Bhatt BM, Mistry S, Pednekar SJ, Chari VV, Desai SA, Joshi JM, Paidhungat AJ (2009) Lung epithelial permeability and inhaled furosemide: added dimensions in asthmatics. Ann Nucl Med 23(6):549–557
British Thoracic Society (2006) Burden of lung disease – a statistics report from British Thoracic Society 2006, 2nd edn. Available from https://www.brit-thoracic.org.uk/document-library/delivery-of-respiratory-care/burden-of-lung-disease/burden-of-lung-disease-2006. Accessed 12 Aug 2015
Brozmanova M, Calkovsky V, Plevkova J, Bartos V, Plank L, Tatar M (2006) Early and late allergic phase related cough response in sensitized Guinea Pigs with experimental allergic rhinitis. Physiol Res 55(5):577–584
Brozmanova M, Plevkova J, Tatar M, Kollarik M (2008) Cough reflex sensitivity is increased in the guinea pig model of allergic rhinitis. J Physiol Pharmacol 59(Suppl 6):153–161
Chapman MD, Wünschmann S, Pomés A (2007) Proteases as Th2 adjuvants. Curr Allergy Asthma Rep 7(5):363–367
Chow PKH, Ng RTH, Ogden BE (2008) Using animal models in biomedical research: a primer for the investigator. World Scientific, Hackensack
Hammad H, Plantinga M, Deswarte K, Pouliot P, Willart MA, Kool M, Muskens F, Lambrecht BN (2010) Inflammatory dendritic cells – not basophils – are necessary and sufficient for induction of Th2 immunity to inhaled house dust mite allergen. J Exp Med 207(10):2097–2111
Hori A, Fujimura M, Ohkura N, Tokuda A (2011) Involvement of nitric oxide (NO) in cough reflex sensitivity between non-sensitized and OVA-sensitized guinea pigs. Cough 7(1):5
Kumar RK, Herbert C, Foster PS (2008) The “classical” ovalbumin challenge model of asthma in mice. Curr Drug Targets 9(6):485–494
Mokry J, Joskova M, Mokra D, Christensen I, Nosalova G (2013) Effects of selective inhibition of PDE4 and PDE7 on airway reactivity and cough in healthy and ovalbumin-sensitized guinea pigs. Adv Exp Med Biol 756:57–64
Nials AT, Uddin S (2008) Mouse models of allergic asthma: acute and chronic allergen challenge. Dis Model Mech 1(4–5):213–220
Pennock BE, Cox CP, Rogers RM, Cain WA, Wells JH (1979) A noninvasive technique for measurement of changes in specific airway resistance. J Appl Physiol Respir Environ Exerc Physiol 46(2):399–406
Poliacek I, Jakus J, Simera M, Barani H, Visnovcova N, Halasova E, Tomori Z (2009a) Excitability and rhythmicity of tracheobronchial cough is altered by aspiration reflex in cats. J Physiol Pharmacol 60(Suppl 5):105–110
Poliacek I, Tomori Z, Simera M, Barani H, Visnovcova N, Halasova E, Donic V, Jakus J (2009b) Provocation of aspiration reflexes and their effects on the pattern of cough and reflex apnea in cats. J Physiol Pharmacol 60(Suppl 5):99–104
Ruoppi P, Koistinen T, Pennanen S (2005) Sensitisation to mites in laboratory animal workers with rhinitis. Occup Environ Med 62(9):612–615
Stewart GA, Boyd SM, Bird CH, Krska KD, Kollinger MR, Thompson PJ (1994) Immunobiology of the serine protease allergens from house dust mites. Am J Ind Med 25(1):105–107
Sutovska M, Adamkov M, Kocmalova M, Mesarosova L, Oravec M, Franova S (2013) CRAC ion channels and airway defense reflexes in experimental allergic inflammation. Adv Exp Med Biol 756:39–48
Turi GJ, Ellis R, Wattie JN, Labiris NR, Inman MD (2011) The effects of inhaled house dust mite on airway barrier function and sensitivity to inhaled methacholine in mice. Am J Physiol Lung Cell Mol Physiol 300(2):L185–L190
Wan H, Winton HL, Soeller C, Tovey ER, Gruenert DC, Thompson PJ, Stewart GA, Taylor GW, Garrod DR, Cannell MB, Robinson C (1999) Der p 1 facilitates transepithelial allergen delivery by disruption of tight junctions. J Clin Invest 104(1):123–133
Wan H, Winton HL, Soeller C, Taylor GW, Gruenert DC, Thompson PJ, Cannell MB, Stewart GA, Garrod DR, Robinson C (2001) The transmembrane protein occludin of epithelial tight junctions is a functional target for serine peptidases from faecal pellets of Dermatophagoides pteronyssinus. Clin Exp Allergy 31(2):279–294
Yasue M, Yokota T, Suko M, Okudaira H, Okumura Y (1998) Comparison of sensitization to crude and purified house dust mite allergens in inbred mice. Lab Anim Sci 48(4):346–352
Acknowledgements
This study was supported by VEGA No. 1/0107/2014 and Biomed ITMS: 26220220187
Conflicts of Interest
The authors declare no conflicts of interest in relation to this article.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer International Publishing Switzerland
About this chapter
Cite this chapter
Buday, T., Gavliakova, S., Mokry, J., Medvedova, I., Kavalcikova-Bogdanova, N., Plevkova, J. (2016). The Guinea Pig Sensitized by House Dust Mite: A Model of Experimental Cough Studies. In: Pokorski, M. (eds) Respiratory Contagion. Advances in Experimental Medicine and Biology(), vol 905. Springer, Cham. https://doi.org/10.1007/5584_2016_217
Download citation
DOI: https://doi.org/10.1007/5584_2016_217
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-30603-2
Online ISBN: 978-3-319-30604-9
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)