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The Lactobacillus plantarum Eno A1 Enolase Is Involved in Immunostimulation of Caco-2 Cells and in Biofilm Development

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Advances in Microbiology, Infectious Diseases and Public Health

Part of the book series: Advances in Experimental Medicine and Biology ((AMIDPH,volume 897))

Abstract

The role of probiotics in prevention and treatment of a variety of diseases is now well assessed. The presence of adhesive molecules on the cell surface of probiotics has been related to the ability to confer health benefit to the host. We have previously shown that the enolase EnoA1 of Lactobacillus plantarum, one of the most predominant species in the gut microbiota of healthy individuals, is cell surface-expressed and is involved in binding with human fibronectin and plasminogen. By means of comparative analysis between L. plantarum LM3 (wild type) and its isogenic LM3-CC1 (ΔenoA1) mutant strain, here we show that EnoA1 affects the ability of this bacterium to modulate immune response as determined by analysis of expression of immune system molecules in Caco-2 cells. Indeed, we observed induction of TLR2 expression in cells exposed to L. plantarum LM3, while no induction was detectable in cells exposed to LM3-CC1. This difference was much less consistent when expression of TLR4 was determined in cells exposed to the two strains. Pro-inflammatory (IL-6) and anti-inflammatory cytokines (IL-10, TGF-β), and the antimicrobial peptide HBD-2 were induced in Caco-2 cells exposed to L. plantarum LM3, while lower levels of induction were detected in cells exposed to LM3-CC1. We also analyzed the ability to develop biofilm of the two strains, and observed a decrease of about 65 % in the development of mature biofilm in LM3-CC1 compared to the wild type.

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Competing Interests

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Department of Environmental, Biological, and Pharmaceutical Science and Technology, Second University of Naples, via Vivaldi n 43, Caserta, 81100, Italy

    Valeria Vastano, Gianluca Merola & Margherita Sacco

  2. Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology, Second University of Naples, via De Crecchio n 7, Naples, 80138, Italy

    Annunziata Pagano, Alessandra Fusco & Giovanna Donnarumma

Authors
  1. Valeria Vastano
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  2. Annunziata Pagano
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  3. Alessandra Fusco
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  4. Gianluca Merola
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  5. Margherita Sacco
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  6. Giovanna Donnarumma
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Corresponding author

Correspondence to Giovanna Donnarumma .

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Editors and Affiliations

  1. Microbial Biofilm Laboratory, Fondazione Santa Lucia IRCCS, Rome, Italy

    Gianfranco Donelli

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Vastano, V., Pagano, A., Fusco, A., Merola, G., Sacco, M., Donnarumma, G. (2015). The Lactobacillus plantarum Eno A1 Enolase Is Involved in Immunostimulation of Caco-2 Cells and in Biofilm Development. In: Donelli, G. (eds) Advances in Microbiology, Infectious Diseases and Public Health. Advances in Experimental Medicine and Biology(), vol 897. Springer, Cham. https://doi.org/10.1007/5584_2015_5009

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