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Age and Gender-Related Changes in Biogenic Amine Metabolites in Cerebrospinal Fluid in Children

Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 878)

Abstract

Metabolites of cerebrospinal biogenic amines (dopamine and serotonin) are an important tool in clinical research and diagnosis of children with neurotransmitter disorders. In this article we focused on finding relationships between the concentration of biogenic amine metabolites, age, and gender. We analyzed 148 samples from children with drug resistant seizures of unknown etiology and children with mild stable encephalopathy aged 0–18 years. A normal profile of biogenic amines was found in 107 children and those children were enrolled to the study group. The CSF samples were analyzed by HPLC with an electrochemical detector. The concentrations of the dopamine and serotonin metabolites homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA), respectively, were high at birth, gradually decreasing afterward until the 18 years of age. Nevertheless, the HVA/5-HIAA ratio did not vary with age, except in the children below 1 year of age. In the youngest group we observed a strong relationship between the HVA/5-HIAA ratio and age (r = 0.69, p < 0.001). There were no statistical differences in the level of both dopamine and serotonin metabolites between boys and girls, although a trend toward lower HVA and 5-HIAA in the boys was noticeable. Significant inter-gender differences in the level of HVA and 5-HIAA were noted only in the age-group of 1–4 years, with 5-HIAA being higher in the girls than boys (p = 0.004). In conclusion, the study revealed that the concentration of biogenic amine metabolites is age and sex dependent.

Keywords

Biogenic amines Brain metabolism Cerebrospinal fluid Children Gender Neurotransmitters 

Notes

Acknowledgments

The study was supported in part by MNiSW grant N406 030 31/0915.

Conflicts of Interest

The authors declare no conflicts of interest in relation to this article.

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  1. 1.Department of Newborn Screening LaboratoryInstitute of Mother and ChildWarsawPoland
  2. 2.Department of Experimental and Clinical NeuropathologyMedical Research Center, Polish Academy of SciencesWarsawPoland
  3. 3.Department of Child PsychiatryMedical University of WarsawWarsawPoland
  4. 4.Department of Pediatrics, Nutrition and Metabolic DiseasesThe Children Memorial InstituteWarsawPoland
  5. 5.Department of Neurology and EpileptologyThe Children Memorial InstituteWarsawPoland
  6. 6.Clinic of Child and Adolescent NeurologyInstitute of Mother and ChildWarsawPoland
  7. 7.Department of Medical GeneticsThe Children Memorial InstituteWarsawPoland
  8. 8.Department of Laboratory Diagnostics and Clinical Immunology of Developmental AgeMedical University of WarsawWarsawPoland

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