Abstract
Nitric oxide (NO) effects in airways are influenced by the activity of NO-synthase isoforms and NO metabolism. Inducible NO-synthase (iNOS), which produces large amounts of NO, is active during the inflammatory process. NO quickly reacts, producing reactive oxygen species (ROS). In this study we attempted to detect the expression of iNOS and markers of ROS in the airway hyperreactivity (AHR) condition. The study was performed in guinea pigs, divided into four groups. Two groups were treated with the non-selective inhibitor of NO-synthase L-NAME. The other two groups were used as controls. Exhaled NO was monitored in vivo, AHR was assessed both in vivo and in vitro, and the expression of iNOS in lung homogenate, and oxidative stress markers were measured in the venous blood. L-NAME significantly affected the AHR only in in vitro condition, blocked the expression of iNOS in control but not in sensitized animals, and decreased the level of exhaled NO. The results concerning the oxidative stress markers are equivocal. The study confirmed that NO is involved in the regulation of AHR; the effects being mediated via iNOS and ROS activity.
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Acknowledgments
This work was supported by the project “The increasing opportunities for career growth in research and development in the medical sciences”, co-financed from the EU sources: Grant MZ SR 2007/46-UK-11 and VEGA 1/0062/13.
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The authors declare no conflicts of interest in relation to this article.
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Antošová, M., Strapková, A., Mikolka, P., Mokrý, J., Medveďová, I., Mokrá, D. (2014). The Influence of L-NAME on iNOS Expression and Markers of Oxidative Stress in Allergen-Induced Airway Hyperreactivity. In: Pokorski, M. (eds) Allergens and Airway Hyperreactivity. Advances in Experimental Medicine and Biology(), vol 838. Springer, Cham. https://doi.org/10.1007/5584_2014_62
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DOI: https://doi.org/10.1007/5584_2014_62
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