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Fluorescent Methods to Study Biological Membranes pp 417–437Cite as

Application of Quantitative Fluorescence Microscopic Approaches to Monitor Organization and Dynamics of the Serotonin1A Receptor

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Part of the book series: Springer Series on Fluorescence ((SS FLUOR,volume 13))

Abstract

G protein-coupled receptors (GPCRs) are the largest class of molecules involved in signal transduction across membranes and represent major targets in the development of novel drug candidates in all clinical areas. Recent advances in understanding of the nonrandom distribution of GPCRs, G proteins, and effector molecules have given rise to new challenges and complexities in cellular signaling by GPCRs. In this article, we provide specific examples on the application of quantitative fluorescence microscopic approaches to monitor organization and dynamics of the serotonin1A receptor (a GPCR) in live cells. This assumes broader relevance due to the emerging theme that GPCR function depends on its organization and dynamics. We envisage that with progress in understanding of receptor organization and dynamics, our knowledge of GPCR function would improve considerably, thereby enabling to design better therapeutic strategies to combat diseases related to malfunctioning of GPCRs.

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Abbreviations

Bodipy-FL PC:

2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexadecanoyl-sn-glycero-3-phosphocholine

CD:

Cytochalasin D

DRM:

Detergent-resistant membrane

EYFP:

Enhanced yellow fluorescent protein

F-actin:

Filamentous actin

FCS:

Fluorescence correlation spectroscopy

FPR:

N-formyl peptide receptor

FRET:

Fluorescence resonance energy transfer

G-actin:

Globular actin

GFP:

Green fluorescent protein

GPCR:

G protein-coupled receptor

Jas:

Jasplakinolide

LatA:

Latrunculin A

MβCD:

Methyl-β-cyclodextrin

p-MPPI:

4-(2′-Methoxy)-phenyl-1-[2′-(N-2′′-pyridinyl)-p-iodobenzamido]ethyl-piperazine

zFCS:

Z-Scanning FCS

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Acknowledgments

Work in A.C.’s laboratory was supported by the Council of Scientific and Industrial Research and Department of Science and Technology, Govt. of India. We thank Sourav Ganguly for help in making Fig. 2. A.C. is an adjunct professor at the Special Centre for Molecular Medicine of Jawaharlal Nehru University (New Delhi, India) and Indian Institute of Science Education and Research (Mohali, India) and an honorary professor of the Jawaharlal Nehru Centre for Advanced Scientific Research (Bangalore, India). A.C. gratefully acknowledges J.C. Bose Fellowship (Department of Science and Technology, Govt. of India). Some of the work described in this article was carried out by former members of A.C.’s research group whose contributions are gratefully acknowledged. We thank members of our laboratory for critically reading the manuscript.

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  1. Centre for Cellular and Molecular Biology, Council of Scientific and Industrial Research, Hyderabad, 500 007, India

    Md. Jafurulla & Amitabha Chattopadhyay

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Correspondence to Amitabha Chattopadhyay .

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  1. Faculté de Pharmacie, Laboratoire de Biophotonique, Université de Strasbourg, route du Rhin 74, Illkirch, 67401, France

    Yves Mély

  2. Faculté de Pharmacie, Laboratoire de Biophotonique et, Université de Strasbourg, route du Rhin 74, Illkirch, 67401, France

    Guy Duportail

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Jafurulla, M., Chattopadhyay, A. (2012). Application of Quantitative Fluorescence Microscopic Approaches to Monitor Organization and Dynamics of the Serotonin1A Receptor. In: Mély, Y., Duportail, G. (eds) Fluorescent Methods to Study Biological Membranes. Springer Series on Fluorescence, vol 13. Springer, Berlin, Heidelberg. https://doi.org/10.1007/4243_2012_58

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