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Fluorescent Methods to Study Biological Membranes pp 389–415Cite as

New Fluorescent Strategies Shine Light on the Evolving Concept of GPCR Oligomerization

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Part of the book series: Springer Series on Fluorescence ((SS FLUOR,volume 13))

Abstract

GPCR oligomerization has been a matter of intense research these last years. FRET and BRET methods have paved the way to a generalized concept of potential GPCR oligomerization in artificial systems (transfected cell lines). More recently, the use of fluorescent ligands compatible with time-resolved FRET studies has opened the possibility of GPCR oligomerization study in their native context and brought evidence of their existence. Furthermore, recent applications of original fluorescence techniques are unveiling new information on the dynamics that govern these complexes and are changing the way we see GPCR oligomeric structures.

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Abbreviations

ACP:

Acyl carrier protein

BiFC:

Bimolecular fluorescence complementation

BRET:

Bioluminescence resonance energy transfer

CFP:

Cyan fluorescent protein

co-IP:

Co-immunoprecipitation

FCS:

Fluorescence correlation spectroscopy

FP:

Fluorescent protein

FRET:

Fluorescence resonance energy transfer

GABA:

γ-Aminobutyric acid

GFP:

Green fluorescent protein

GPCR:

G-protein-coupled receptor

hAGT:

O6-Alkylguanine-DNA alkyltransferase

HTS:

High-throughput screening

pbFRET:

Photobleaching FRET

RET:

Resonance energy transfer

Rluc:

Renilla luciferase

RTK:

Receptor tyrosine kinase

smFRET:

Single-molecule FRET

SPT:

Single-particle tracking

TIRF:

Total internal reflection fluorescence

TR-FRET:

Time-resolved FRET

YFP:

Yellow fluorescent protein

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Authors and Affiliations

  1. Institut de Génomique Fonctionnelle, Centre National de la Recherche Scientifique, Montpellier, France

    Martin Cottet, Orestis Faklaris, Jean-Philippe Pin & Thierry Durroux

  2. Institut National de la Santé et de la Recherche Médicale, Montpellier, France

    Martin Cottet, Orestis Faklaris, Jean-Philippe Pin & Thierry Durroux

  3. Université Montpellier 1 and 2, Montpellier, France

    Martin Cottet, Orestis Faklaris, Jean-Philippe Pin & Thierry Durroux

  4. Cisbio Bioassays, Bagnols sur Cèze, France

    Eric Trinquet

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  1. Martin Cottet
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  2. Orestis Faklaris
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  3. Eric Trinquet
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  4. Jean-Philippe Pin
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  5. Thierry Durroux
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Correspondence to Martin Cottet or Thierry Durroux .

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  1. Faculté de Pharmacie, Laboratoire de Biophotonique, Université de Strasbourg, route du Rhin 74, Illkirch, 67401, France

    Yves Mély

  2. Faculté de Pharmacie, Laboratoire de Biophotonique et, Université de Strasbourg, route du Rhin 74, Illkirch, 67401, France

    Guy Duportail

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Cottet, M., Faklaris, O., Trinquet, E., Pin, JP., Durroux, T. (2012). New Fluorescent Strategies Shine Light on the Evolving Concept of GPCR Oligomerization. In: Mély, Y., Duportail, G. (eds) Fluorescent Methods to Study Biological Membranes. Springer Series on Fluorescence, vol 13. Springer, Berlin, Heidelberg. https://doi.org/10.1007/4243_2012_54

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