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Standardization and Quality Assurance in Fluorescence Measurements I pp 415–428Cite as

Total Internal Reflection Fluorescence Sensing – Quality Assurance and Application to Water Analysis

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Part of the book series: Springer Series on Fluorescence ((SS FLUOR,volume 5))

Abstract

Based on the water directives of the European Union, the results of a biosensor system using total internal reflection fluorescence (TIRF) are demonstrated. The optical principal, the assay type, as well as the calibration and validation parameters are discussed. Results for endocrine disrupting compounds are given, and the validation parameters are outlined. These are applied to a measurement in comparison to a collaborative test in cooperation with accredited water laboratories using high performace liquid chromatography (HPLC) and gas chromatography (GC) techniques.

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Authors and Affiliations

  1. Eberhard-Karls Universität, Institut für Physikalische und Theoretische Chemie, Auf der Morgenstelle 8, 72076, Tübingen, Germany

    Guenter Gauglitz & Guenther Proll

Authors
  1. Guenter Gauglitz
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  2. Guenther Proll
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Corresponding authors

Correspondence to Guenter Gauglitz or Guenther Proll .

Editor information

Ute Resch-Genger

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© 2008 Springer-Verlag Berlin Heidelberg

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Gauglitz, G., Proll, G. (2008). Total Internal Reflection Fluorescence Sensing – Quality Assurance and Application to Water Analysis. In: Resch-Genger, U. (eds) Standardization and Quality Assurance in Fluorescence Measurements I. Springer Series on Fluorescence, vol 5. Springer, Berlin, Heidelberg. https://doi.org/10.1007/4243_2008_053

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