Abstract
Characterization of fluorescence imaging systems from the manufacturer's view creates several challenges. What are the key parameters for which characterization is appropriate? How can the standardization procedures developed for use during manufacture be applied during installation and application? With so many instrument variables, how can procedures be developed that give precise diagnostic information? These are not simply questions of “standardized tests”. There are also issues of finding shared confidence in the tests amongst the different users of the systems. Ideally such tests should also allow objective comparison of the performance of systems of different design or from different manufacturers.
This chapter first discusses the factors that affect performance of fluorescence imaging systems and for which standardization tests are required. In many cases the performance in one respect is inter-dependent on the performance in another. The need to develop tests that uncouple these dependencies is discussed.
The chapter then discusses in more detail the particular issue of signal detection sensitivity and the development of standardized tests that are usable and acceptable both during manufacture and for demonstration of performance during installation and ongoing use of the instrument. It is shown that featureless test samples have significant advantages. They enable a range of performance tests to be made with a single sample in a way that is equally accessible to the manufacturer and end user.
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References
Rost FWD (1991) Quantitative fluorescence microscopy. Cambridge University Press, Cambridge
Zwier JM, Van Rooij GJ, Hofstraat JW, Brakenhoff GJ (2004) J Microsc 216(Pt1):15
Zucker RM (2002) Microsc Tod 10(6):20
Zucker RM (2002) Microsc Tod 10(7):8
Hellmuth E, Mühlfriedel W (1996) Zeiss 1846–1905. Vom Atelier für Mechanik zum führenden Unternehmen des optischen Gerätebaus. Böhlau, Köln
Abbe E (1873) Arch Mikros Anat 9:413
McCarthy NJ, Evan GI (1998) Curr Top Dev Biol 36:259
Dunn KW, Mayor S, Myers JN, Maxfield FR (1994) FASEB J 8(9):573
Berland KM (2004) Methods Mol Biol 261:383
Hiraoka Y, Shimi T, Haraguchi T (2002) Cell Struct Funct 27:367
Dickinson ME, Bearman G, Tille S, Lansford R, Fraser SE (2001) Biotechniques 31(6):1272
Dickinson ME, Simbürger E, Zimmermann B, Waters CW, Fraser SE (2003) J Biomed Opt 8:329
Wouters FS, Verveer PJ, Bastiaens IH (2001) Trends Cell Biol (11):5
Kiyokawa E, Hara S, Nakamura T, Matsuda M (2006) Cancer Sci 97(1):8
Jares-Erijman EA, Jovin TM (2003) Nat Biotechnol 21(11):1387
Grunwald D, Cardoso MC, Leonhardt H, Buschmann V (2005) Curr Pharm Biotechnol 6(5):381
Kohl T, Schwille P (2005) Adv Biochem Eng Biotechnol 95:107
Gosch M, Rigler R (2005) Adv Drug Deliv Rev 57(1):169
Houtsmuller AB (2005) Adv Biochem Eng Biotechnol 95:177
Koster M, Frahm T, Hauser H (2005) Curr Opin Biotechnol 1:28
Mullineaux CW (2004) J Exp Bot 55(400):1207
Sprague BL, McNally JG (2005) Trends Cell Biol 15(2):84
Van Drogen F, Peter M (2001) Biol Cell 93(1–2):63
Pawley J (2000) BioTechniques 28(5):884
Zucker RM, Price O (2001) Cytometry 44(4):273
Henderson LO, Marti GE, Gaigalas A, Hannon WH, Vogt RF (1998) Cytometry 33:97
Schwartz A, Marti GE, Poon R, Gratama JW, Fernandez-Repollet E (1998) Cytometry 33:106
Schwartz A, Fernandez-Repollet E, Vogt R, Gratama JW (1996) Cytometry 26:22
Shapiro HM (1995) Practical Flow Cytometry. Wiley-Liss, New York
Chase ES, Hoffman RA (1998) Cytometry 33:267
Hoffman RA (2001) Methods in Cell Biology Vol. 63: Standardization and Quantitation in Flow Cytometry. Academic Press, New York
Wolf F, Geley S (2006) J Microsc 221(Pt1):72
Brakenhoff GJ, Wurpel GWH, Jalink K, Brocks L, Zwier JM (2005) J Microsc 219(Pt3):122
Stelzer EHK (1998) J Microsc 189(Pt1):15–24
Zucker RM (2005) Meth Mol Biol 319:77–136
Shotton DM (1995) Electronic light microscopy. Histochem Cell Biol 104:907–137
Sheppard CJR, Shotton DM (1997) Confocal Laser Scanning Microscopy. Springer, New York, p 9
Hell SW, Stelzer EHK (1995) Handbook of Biological Confocal Microscopy. Plenum Press, New York, pp 347–354
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Dixon, A., Heinlein, T., Wolleschensky, R. (2008). Need for Standardization of Fluorescence Measurements from the Instrument Manufacturer's View. In: Resch-Genger, U. (eds) Standardization and Quality Assurance in Fluorescence Measurements II. Springer Series on Fluorescence, vol 6. Springer, Berlin, Heidelberg. https://doi.org/10.1007/4243_2008_026
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DOI: https://doi.org/10.1007/4243_2008_026
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