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Impact of the Immunological Synapse on T Cell Signaling

  • Michael L. DustinEmail author
Chapter
Part of the Results and Problems in Cell Differentiation book series (RESULTS, volume 43)

Abstract

T cell activation requires interactions of T cell antigen receptors and peptides presented by major histocompatibility complex molecules in an adhesive junction between the T cell and antigen-presenting cell (APC). Stable junctions with bull's-eye supramolecular activation clusters have been defined as immunological synapses (IS). These structures maintain T cell–APC interaction and allow directed secretion. T cells can also be activated by asymmetric hemisynapses (HS) that allow migration during signal integration. IS and HS dominate in different stages of T cell priming. Optimal effector functions may also depend upon cyclical use of IS and HS.

Immunological synapses Signaling Intravital microscopy Cell migration Autoimmunity 

Abbreviations

APC

Antigen-presenting cell

CFP

Cyan fluorescent protein

CFSE

Carboxyfluorescein succinimidyl ester

CNS

Central nervous system

DC

Dendritic cell

eGFP

Enhanced green fluorescent protein

HEV

High endothelial venules

HS

Hemisynapse

ICAM

Intercellular adhesion molecule

IS

Immunological synapse

LFA

Lymphocyte function associated

MBP

Myelin basic protein

MHCp

Major histocompatibility complex–peptide complex

NKT

Natural killer T cell

SMAC

Supramolecular activation cluster

TCR

T cell receptor

TIRFM

Total internal reflection fluorescence microscopy

TPLSM

Two-photon laser scanning microscopy

YFP

Yellow fluorescent protein

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Notes

Acknowledgments

I thank R. Varma, G. Campi, T. Sims, T. Cameron, J. Kim, G. Shakhar, and M. Nussenzweig for valuable discussions.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2006

Authors and Affiliations

  1. 1.Program in Molecular Pathogenesis, Skirball Institute of Biomolecular Medicine, and Department of PathologyNew York University School of MedicineNew YorkUSA

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